1887

Abstract

Phosphoprotein P of rinderpest virus (RPV), when expressed in , is present in the unphosphorylated form. Bacterially expressed P protein was phosphorylated by a eukaryotic cellular extract, and casein kinase II (CK II) was identified as the cellular kinase involved in phosphorylation. phosphorylation of P-deletion mutants identified the N terminus as a phosphorylation domain. phosphorylation of single or multiple serine mutants of P protein identified serine residues at 49, 88 and 151 as phospho-acceptor residues. The role of P protein phosphorylation in virus replication/transcription was evaluated using the RPV minigenome system and replication/transcription of a reporter gene . P protein phosphorylation was shown to be essential for replication/transcription since phosphorylation-null mutants do not support expression of a reporter gene. Transfection of increased amounts of phosphorylation-null mutant did not support minigenome replication/transcription .

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.19702-0
2004-03-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/jgv/85/3/vir850687.html?itemId=/content/journal/jgv/10.1099/vir.0.19702-0&mimeType=html&fmt=ahah

References

  1. Barik S., Banerjee A. K. 1991; Cloning and expression of the vesicular stomatitis virus phosphoprotein gene in Escherichia coli : analysis of phosphorylation status versus transcriptional activity. J Virol 65:1719–1726
    [Google Scholar]
  2. Barik S., Banerjee A. K. 1992a; Phosphorylation by cellular casein kinase II is essential for transcriptional activity of vesicular stomatitis virus phosphoprotein P. Proc Natl Acad Sci U S A 89:6570–6574
    [Google Scholar]
  3. Barik S., Banerjee A. K. 1992b; Sequential phosphorylation of the phosphoprotein of vesicular stomatitis virus by cellular and viral protein kinases is essential for transcription activation. J Virol 66:1109–1118
    [Google Scholar]
  4. Baron M. D., Barrett T. 1997; Rescue of rinderpest virus from cloned cDNA. J Virol 71:1265–1271
    [Google Scholar]
  5. Basak S., Raha T., Chattopadhyay D., Majumder A., Shaila M. S., Chattopadhyay D. J. 2003; Leader RNA binding ability of Chandipura virus P protein is regulated by its phosphorylation status: a possible role in genome transcription-replication switch. Virology 307:372–385
    [Google Scholar]
  6. Chattopadhyay D., Raha T., Chattopadhyay D. 1997; Single serine phosphorylation within the acidic domain of Chandipura virus P protein regulates the transcription in vitro. Virology 239:11–19
    [Google Scholar]
  7. Curran J., Pelet T., Kolakofsky D. 1994; An acidic activation-like domain of the Sendai virus P protein is required for RNA synthesis and encapsidation. Virology 202:875–884
    [Google Scholar]
  8. Das T., Schuster A., Schneider-Schaulies S., Banerjee A. K. 1995; Involvement of cellular casein kinase II in the phosphorylation of measles virus P protein: identification of phosphorylation sites. Virology 211:218–226
    [Google Scholar]
  9. Einberger H., Mertz R., Hofschneider P. H., Neubert W. J. 1990; Purification, renaturation, and reconstituted protein kinase activity of the Sendai virus large (L) protein: L protein phosphorylates the NP and P proteins in vitro. J Virol 64:4274–4280
    [Google Scholar]
  10. Emerson S. U., Schubert M. 1987; Location of the binding domains for the RNA polymerase L and the ribonucleocapsid template within different halves of the NS phosphoprotein of vesicular stomatitis virus. Proc Natl Acad Sci U S A 84:5655–5659
    [Google Scholar]
  11. Gao Y., Lenard J. 1995; Multimerization and transcriptional activation of the phosphoprotein (P) of vesicular stomatitis virus by casein kinase-II. EMBO J 14:1240–1247
    [Google Scholar]
  12. Ghosh A., Joshi V. D., Shaila M. S. 1995; Characterization of an in vitro transcription system from rinderpest virus. Vet Microbiol 44:165–173
    [Google Scholar]
  13. Huber M., Cattaneo R., Spielhofer P., Orvell C., Norrby E., Messerli M., Perriard J. C., Billeter M. A. 1991; Measles virus phosphoprotein retains the nucleocapsid protein in the cytoplasm. Virology 185:299–308
    [Google Scholar]
  14. Huntley C. C., De B. P., Banerjee A. K. 1997; Phosphorylation of Sendai virus phosphoprotein by cellular protein kinase C ζ . J Biol Chem 272:16578–16584
    [Google Scholar]
  15. Liu Z., Huntley C. C., De B. P., Das T., Banerjee A. K., Oglesbee M. J. 1997; Phosphorylation of canine distemper virus P protein by protein kinase C- ζ and casein kinase II. Virology 232:198–206
    [Google Scholar]
  16. Mathur M., Das T., Chen J. L., Chattopadhyay D., Banerjee A. K. 1997; Display of disparate transcription phenotype by the phosphorylation negative P protein mutants of vesicular stomatitis virus, Indiana serotype, expressed in E. coli and eukaryotic cells. Gene Expr 6:275–286
    [Google Scholar]
  17. Mazumder B., Barik S. 1994; Requirement of casein kinase II-mediated phosphorylation for the transcriptional activity of human respiratory syncytial viral phosphoprotein P: transdominant negative phenotype of phosphorylation-defective P mutants. Virology 205:104–111
    [Google Scholar]
  18. Pattnaik A. K., Hwang L., Li T., Englund N., Mathur M., Das T., Banerjee A. K. 1997; Phosphorylation within the amino-terminal acidic domain I of the phosphoprotein of vesicular stomatitis virus is required for transcription but not for replication. J Virol 71:8167–8175
    [Google Scholar]
  19. Takacs A. M., Barik S., Das T., Banerjee A. K. 1992; Phosphorylation of specific serine residues within the acidic domain of the phosphoprotein of vesicular stomatitis virus regulates transcription in vitro. J Virol 66:5542–5548
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.19702-0
Loading
/content/journal/jgv/10.1099/vir.0.19702-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error