RT Journal Article SR Electronic(1) A1 Jiang, Shengjun A1 Ji, Shaozhong A1 Tang, Qing A1 Cui, Xiaoying A1 Yang, Hongyang A1 Kan, Biao A1 Gao, ShouyiYR 2008 T1 Molecular characterization of a novel adult diarrhoea rotavirus strain J19 isolated in China and its significance for the evolution and origin of group B rotaviruses JF Journal of General Virology, VO 89 IS 10 SP 2622 OP 2629 DO https://doi.org/10.1099/vir.0.2008/001933-0 PB Microbiology Society, SN 1465-2099, AB The complete genome of a novel adult diarrhoea rotavirus strain J19 was cloned and sequenced using an improved single-primer sequence-independent method. The complete genome is 17 961 bp and is AU-rich (66.49 %). Northern blot analysis and genomic sequence analysis indicated that segments 1–11 encode 11 viral proteins, respectively. Protein alignments with the corresponding proteins of J19 with B219, and groups A, B and C rotaviruses, produced higher per cent sequence identities to B219. Among groups A, B and C rotaviruses, 10 proteins from group B rotaviruses exhibited slightly higher amino acid sequence identity to the J19 proteins, but proteins of J19 showed low amino acid sequence identity with groups A and C rotaviruses. Construction of unrooted phylogenetic trees using a set of known proteins and representatives of three known rotavirus groups revealed that six structural proteins were positioned close to B219 and the basal nodes of groups A, B and C lineages, although with a preferred association with group B lineages. Phylogenetic analysis of the five non-structural proteins showed a similar trend. The results of the serological analysis, protein sequence analysis and phylogenetic analysis suggested that J19 would be a novel rotavirus strain with great significance to the evolution and origin of group B rotaviruses., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.2008/001933-0