Mapping the minimal regions within the ORF73 protein required for herpesvirus saimiri episomal persistence
Griffiths, Rhoswyn and Harrison, Sally M. and Macnab, Stuart and Whitehouse, Adrian,, 89, 2843-2850 (2008),
doi = https://doi.org/10.1099/vir.0.2008/002725-0,
publicationName = Microbiology Society,
issn = 0022-1317,
abstract= Herpesvirus saimiri (HVS) establishes a persistent infection in which the viral genome persists as a circular non-integrated episome. ORF73 tethers HVS episomes to host mitotic chromosomes, allowing episomal persistence via an interaction with the chromosome-associated protein, MeCP2. Here we demonstrate that ORF73 also interacts with the linker histone H1 via its C terminus, suggesting it associates with multiple chromosome-associated proteins. In addition, we show that the C terminus is also required for the ability of ORF73 to bind the terminal repeat region of the HVS genome. These results suggest that the ORF73 C terminus contains all the necessary elements required for HVS episomal persistence. Using a range of ORF73 C terminus deletions to rescue the episomal maintenance properties of a HVSΔ73 recombinant virus, we show that a C terminus region comprising residues 285–407 is sufficient to maintain the HVS episome in a dividing cell population.,
language=,
type=