Suppression of short interfering RNA-mediated gene silencing by the structural proteins of hepatitis C virus
Ji, Jingmin and Glaser, Andrea and Wernli, Marion and Berke, Jan Martin and Moradpour, Darius and Erb, Peter,, 89, 2761-2766 (2008),
doi = https://doi.org/10.1099/vir.0.2008/002923-0,
publicationName = Microbiology Society,
issn = 0022-1317,
abstract= Viruses have evolved strategies to overcome the antiviral effects of the host at different levels. Besides specific defence mechanisms, the host responds to viral infection via the interferon pathway and also by RNA interference (RNAi). However, several viruses have been identified that suppress RNAi. We addressed the question of whether hepatitis C virus (HCV) suppresses RNAi, using cell lines constitutively expressing green fluorescent protein (GFP) and inducibly expressing HCV proteins. It was found that short interfering RNA-mediated GFP gene silencing was inhibited when the entire HCV polyprotein was expressed. Further studies showed that HCV structural proteins, and in particular envelope protein 2 (E2), were responsible for this inhibition. Co-precipitation assays demonstrated that E2 bound to Argonaute-2 (Ago-2), a member of the RNA-induced silencing complex, RISC. Thus, HCV E2 that interacts with Ago-2 is able to suppress RNAi.,
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