1887

Abstract

Parvovirus B19 has been associated with liver dysfunction and has been considered a potential aetiological agent of fulminant hepatitis and hepatitis-associated aplastic anaemia. The possible effects of B19 virus infection on the liver have been investigated using HepG2 hepatocellular carcinoma cells as a model system, but the reported results are inconsistent. To investigate this relationship further, this study followed the course of B19 virus infection of HepG2 cells in terms of viral DNA, RNA and protein production by quantitative PCR, RT-PCR and immunofluorescence assays. The data showed that B19 virus is able to bind and possibly enter HepG2 cells, but that viral genome replication or transcription is not supported and that viral proteins are not produced. As far as HepG2 cells can be considered a representative model system, any possible pathogenic role of B19 virus on the liver cannot be ascribed to infection or to a direct cytopathic effect on hepatocytes.

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2008-12-01
2024-03-28
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References

  1. Abe K., Kiuchi T., Tanaka K., Edamoto Y., Aiba N., Sata T. 2007; Characterization of erythrovirus B19 genomes isolated in liver tissues from patients with fulminant hepatitis and biliary atresia who underwent liver transplantation. Int J Med Sci 4:105–109
    [Google Scholar]
  2. Bonvicini F., Filippone C., Delbarba S., Manaresi E., Zerbini M., Musiani M., Gallinella G. 2006; Parvovirus B19 genome as a single, two-state replicative and transcriptional unit. Virology 347:447–454 [CrossRef]
    [Google Scholar]
  3. Brown K. E., Anderson S. M., Young N. S. 1993; Erythrocyte P antigen: cellular receptor for B19 parvovirus. Science 262:114–117 [CrossRef]
    [Google Scholar]
  4. Caillet-Fauquet P., Di Giambattista M., Draps M. L., Hougardy V., de Launoit Y., Laub R. 2004; An assay for parvovirus B19 neutralizing antibodies based on human hepatocarcinoma cell lines. Transfusion 44:1340–1343 [CrossRef]
    [Google Scholar]
  5. Eis-Hubinger A. M., Reber U., Abdul-Nour T., Glatzel U., Lauschke H., Putz U. 2001; Evidence for persistence of parvovirus B19 DNA in livers of adults. J Med Virol 65:395–401 [CrossRef]
    [Google Scholar]
  6. Gallinella G., Bonvicini F., Filippone C., Delbarba S., Manaresi E., Zerbini M., Musiani M. 2004; Calibrated real-time PCR for evaluation of parvovirus B19 viral load. Clin Chem 50:759–762 [CrossRef]
    [Google Scholar]
  7. Hillingso J. G., Jensen I. P., Tom-Petersen L. 1998; Parvovirus B19 and acute hepatitis in adults. Lancet 351:955–956 [CrossRef]
    [Google Scholar]
  8. Karetnyi Y. V., Beck P. R., Markin R. S., Langnas A. N., Naides S. J. 1999; Human parvovirus B19 infection in acute fulminant liver failure. Arch Virol 144:1713–1724 [CrossRef]
    [Google Scholar]
  9. Langnas A. N., Markin R. S., Cattral M. S., Naides S. J. 1995; Parvovirus B19 as a possible causative agent of fulminant liver failure and associated aplastic anemia. Hepatology 22:1661–1665
    [Google Scholar]
  10. Naides S. J., Karetnyi Y. V., Cooling L. L., Mark R. S., Langnas A. N. 1996; Human parvovirus B19 infection and hepatitis. Lancet 347:1563–1564 [CrossRef]
    [Google Scholar]
  11. Pardi D. S., Romero Y., Mertz L. E., Douglas D. D. 1998; Hepatitis-associated aplastic anemia and acute parvovirus B19 infection: a report of two cases and a review of the literature. Am J Gastroenterol 93:468–470
    [Google Scholar]
  12. Pinho J. R., Alves V. A., Vieira A. F., Moralez M. O., Fonseca L. E., Guz B., Wakamatsu A., Cancado E. L., Carrilho F. J. other authors 2001; Detection of human parvovirus B19 in a patient with hepatitis. Braz J Med Biol Res 34:1131–1138
    [Google Scholar]
  13. Poole B. D., Karetnyi Y. V., Naides S. J. 2004; Parvovirus B19-induced apoptosis of hepatocytes. J Virol 78:7775–7783 [CrossRef]
    [Google Scholar]
  14. Poole B. D., Zhou J., Grote A., Schiffenbauer A., Naides S. J. 2006; Apoptosis of liver-derived cells induced by parvovirus B19 nonstructural protein. J Virol 80:4114–4121 [CrossRef]
    [Google Scholar]
  15. Sokal E. M., Melchior M., Cornu C., Vandenbroucke A. T., Buts J. P., Cohen B. J., Burtonboy G. 1998; Acute parvovirus B19 infection associated with fulminant hepatitis of favourable prognosis in young children. Lancet 352:1739–1741 [CrossRef]
    [Google Scholar]
  16. Tsuda H. 1993; Liver dysfunction caused by parvovirus B19. Am J Gastroenterol 88:1463
    [Google Scholar]
  17. Weigel-Kelley K. A., Yoder M. C., Srivastava A. 2003; α 5 β 1 integrin as a cellular coreceptor for human parvovirus B19: requirement of functional activation of β 1 integrin for viral entry. Blood 102:3927–3933 [CrossRef]
    [Google Scholar]
  18. Wong S., Young N. S., Brown K. E. 2003; Prevalence of parvovirus B19 in liver tissue: no association with fulminant hepatitis or hepatitis-associated aplastic anemia. J Infect Dis 187:1581–1586 [CrossRef]
    [Google Scholar]
  19. Yoto Y., Kudoh T., Haseyama K., Suzuki N., Chiba S. 1996; Human parvovirus B19 infection associated with acute hepatitis. Lancet 347:868–869 [CrossRef]
    [Google Scholar]
  20. Young N. S., Brown K. E. 2004; Parvovirus B19. N Engl J Med 350:586–597 [CrossRef]
    [Google Scholar]
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