1887

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Volume 85, Issue 4

Epidemiological and sequence differences between two subtypes (Ae and Aa) of hepatitis B virus genotype A Free

Abstract

Complete nucleotide sequences of 19 hepatitis B virus (HBV) isolates of genotype A (HBV/A) were determined and analysed along with those of 20 previously reported HBV/A isolates. Of the 19 HBV/A isolates, six including three from Japan and three from the USA clustered with the 14 HBV/A isolates from Western countries. The remaining 13 isolates including four from The Philippines, two from India, three from Nepal and four from Bangladesh clustered with the six HBV/A isolates reported from The Philippines, South Africa and Malawi. Due to distinct epidemiological distributions, genotype A in the 20 HBV isolates was classified into subtype Ae (e for Europe), and that in the other 19 into subtype Aa (a for Asia and Africa) provisionally. The 19 HBV/Aa isolates had a sequence variation significantly greater than that of the 20 HBV/Ae isolates (2·5±0·3 % vs 1·1±0·6 %, <0·0001); they differed by 5·0±0·4 % (4·1–6·4 %). The double mutation (T1762/A1764) in the core promoter was significantly more frequent in HBV/Aa isolates than in HBV/Ae isolates (11/19 or 58 % vs 5/20 or 25 %, <0·01). In the pregenome encapsidation () signal, a point mutation from G to A or T at nt 1862 was detected in 16 of the 19 (84 %) HBV/Aa isolates but not in any of the 20 HBV/Ae isolates, which may affect virus replication and translation of hepatitis B e antigen. Subtypes Aa and Ae of genotype A deserve evaluation for any clinical differences between them, with a special reference to hepatocellular carcinoma prevalent in Africa.

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2004-04-01
2024-04-19
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References

  1. Akuta N., Suzuki F., Kobayashi M. 8 other authors 2003; The influence of hepatitis B virus genotype on the development of lamivudine resistance during long-term treatment. J Hepatol 38:315–321
     [Google Scholar]
  2. Arauz-Ruiz P., Norder H., Robertson B. H., Magnius L. O. 2002; Genotype H: a new Amerindian genotype of hepatitis B virus revealed in Central America. J Gen Virol 83:2059–2073
     [Google Scholar]
  3. Attia M. A. 1998; Prevalence of hepatitis B and C in Egypt and Africa. Antivir Ther 3:1–9
     [Google Scholar]
  4. Bowyer S. M., van Staden L., Kew M. C., Sim J. G. 1997; A unique segment of the hepatitis B virus group A genotype identified in isolates from South Africa. J Gen Virol 78:1719–1729
     [Google Scholar]
  5. Carman W. F., Jacyna M. R., Hadziyannis S., Karayiannis P., McGarvey M. J., Makris A., Thomas H. C. 1989; Mutation preventing formation of hepatitis B e antigen in patients with chronic hepatitis B infection. Lancet 2:588–591
     [Google Scholar]
  6. Carman W. F., Thursz M., Hadziyannis S., McIntyre G., Colman K., Gioustoz A., Fattovich G., Alberti A., Thomas H. C. 1995; Hepatitis B e antigen negative chronic active hepatitis: hepatitis B virus core mutations occur predominantly in known antigenic determinants. J Viral Hepat 2:77–84
     [Google Scholar]
  7. Chu C. J., Hussain M., Lok A. S. 2002; Hepatitis B virus genotype B is associated with earlier HBeAg seroconversion compared with hepatitis B virus genotype C. Gastroenterology 122:1756–1762
     [Google Scholar]
  8. Di Bisceglie A. M., Carithers R. L. Jr, Gores G. J. 1998; Hepatocellular carcinoma. Hepatology 28:1161–1165
     [Google Scholar]
  9. Dusheiko G. M., Bowyer S. M., Sjogren M. H., Ritchie M. J., Santos A. P., Kew M. C. 1985; Replication of hepatitis B virus in adult carriers in an endemic area. J Infect Dis 152:566–571
     [Google Scholar]
  10. Edman J. C., Gray P., Valenzuela P., Rall L. B., Rutter W. J. 1980; Integration of hepatitis B virus sequences and their expression in a human hepatoma cell. Nature 286:535–538
     [Google Scholar]
  11. Grethe S., Heckel J. O., Rietschel W., Hufert F. T. 2000; Molecular epidemiology of hepatitis B virus variants in nonhuman primates. J Virol 74:5377–5381
     [Google Scholar]
  12. Horikita M., Itoh S., Yamamoto K., Shibayama T., Tsuda F., Okamoto H. 1994; Differences in the entire nucleotide sequence between hepatitis B virus genomes from carriers positive for antibody to hepatitis B e antigen with and without active disease. J Med Virol 44:96–103
     [Google Scholar]
  13. Hou J., Lin Y., Waters J. 7 other authors 2002; Detection and significance of a G1862T variant of hepatitis B virus in Chinese patients with fulminant hepatitis. J Gen Virol 83:2291–2298
     [Google Scholar]
  14. Ina Y. 1994; oden: a program package for molecular evolutionary analysis and database search of DNA and amino acid sequences. Comput Appl Biosci 10:11–12
     [Google Scholar]
  15. Kao J. H., Chen P. J., Lai M. Y., Chen D. S. 2002; Genotypes and clinical phenotypes of hepatitis B virus in patients with chronic hepatitis B virus infection. J Clin Microbiol 40:1207–1209
     [Google Scholar]
  16. Kato H., Orito E., Gish R. G. & 7 other authors (2002a). Hepatitis B e antigen in sera from individuals infected with hepatitis B virus of genotype G. Hepatology 35:922–929
     [Google Scholar]
  17. Kato H., Orito E., Gish R. G., Sugauchi F., Suzuki S., Ueda R., Miyakawa Y., Mizokami M. 2002b; Characteristics of hepatitis B virus isolates of genotype G and their phylogenetic differences from the other six genotypes (A through F). J Virol 76:6131–6137
     [Google Scholar]
  18. Kobayashi M., Arase Y., Ikeda K. 11 other authors 2002; Viral genotypes and response to interferon in patients with acute prolonged hepatitis B virus infection of adulthood in Japan. J Med Virol 68:522–528
     [Google Scholar]
  19. Kosaka Y., Takase K., Kojima M. 9 other authors 1991; Fulminant hepatitis B: induction by hepatitis B virus mutants defective in the precore region and incapable of encoding e antigen. Gastroenterology 100:1087–1094
     [Google Scholar]
  20. Kramvis A., Bukofzer S., Kew M. C., Song E. 1997; Nucleic acid sequence analysis of the precore region of hepatitis B virus from sera of southern African black adult carriers of the virus. Hepatology 25:235–240
     [Google Scholar]
  21. Kramvis A., Kew M. C., Bukofzer S. 1998; Hepatitis B virus precore mutants in serum and liver of Southern African Blacks with hepatocellular carcinoma. J Hepatol 28:132–141
     [Google Scholar]
  22. Kramvis A., Weitzmann L., Owiredu W. K., Kew M. C. 2002; Analysis of the complete genome of subgroup A′ hepatitis B virus isolates from South Africa. J Gen Virol 83:835–839
     [Google Scholar]
  23. Laskus T., Persing D. H., Nowicki M. J., Mosley J. W., Rakela J. 1993; Nucleotide sequence analysis of the precore region in patients with fulminant hepatitis B in the United States. Gastroenterology 105:1173–1178
     [Google Scholar]
  24. Li J. S., Tong S. P., Wen Y. M., Vitvitski L., Zhang Q., Trepo C. 1993; Hepatitis B virus genotype A rarely circulates as an HBe-minus mutant: possible contribution of a single nucleotide in the precore region. J Virol 67:5402–5410
     [Google Scholar]
  25. Liang T. J., Hasegawa K., Rimon N., Wands J. R., Ben-Porath E. 1991; A hepatitis B virus mutant associated with an epidemic of fulminant hepatitis. N Engl J Med 324:1705–1709
     [Google Scholar]
  26. Lindh M., Andersson A. S., Gusdal A. 1997; Genotypes, nt 1858 variants, and geographic origin of hepatitis B virus – large-scale analysis using a new genotyping method. J Infect Dis 175:1285–1293
     [Google Scholar]
  27. Loriot M. A., Marcellin P., Talbodec N., Guigonis V., Gigou M., Boyer N., Bezeaud A., Erlinger S., Benhamou J. P. 1995; Low frequency of precore hepatitis B virus mutants in anti-hepatitis B e-positive reactivation after loss of hepatitis B e antigen in patients with chronic hepatitis B. Hepatology 21:627–631
     [Google Scholar]
  28. Magnius L. O., Norder H. 1995; Subtypes, genotypes and molecular epidemiology of the hepatitis B virus as reflected by sequence variability of the S-gene. Intervirology 38:24–34
     [Google Scholar]
  29. Mayerat C., Mantegani A., Frei C. 1999; Does hepatitis B virus (HBV) genotype influence the clinical outcome of HBV infection?. J Viral Hepat 6:299–304
     [Google Scholar]
  30. Mizokami M., Nakano T., Orito E., Tanaka Y., Sakugawa H., Mukaide M., Robertson B. H. 1999; Hepatitis B virus genotype assignment using restriction fragment length polymorphism patterns. FEBS Lett 450:66–71
     [Google Scholar]
  31. Nassal M., Rieger A. 1996; A bulged region of the hepatitis B virus RNA encapsidation signal contains the replication origin for discontinuous first-strand DNA synthesis. J Virol 70:2764–2773
     [Google Scholar]
  32. Noppornpanth S., Haagmans B. L., Bhattarakosol P., Ratanakorn P., Niesters H. G., Osterhaus A. D., Poovorawan Y. 2003; Molecular epidemiology of gibbon hepatitis B virus transmission. J Gen Virol 84:147–155
     [Google Scholar]
  33. Norder H., Courouce A. M., Magnius L. O. 1994; Complete genomes, phylogenetic relatedness, and structural proteins of six strains of the hepatitis B virus, four of which represent two new genotypes. Virology 198:489–503
     [Google Scholar]
  34. Okamoto H., Imai M., Tsuda F., Tanaka T., Miyakawa Y., Mayumi M. 1987; Point mutation in the S gene of hepatitis B virus for a d/y or w/r subtypic change in two blood donors carrying a surface antigen of compound subtype adyr or adwr. J Virol 61:3030–3034
     [Google Scholar]
  35. Okamoto H., Tsuda F., Sakugawa H., Sastrosoewignjo R. I., Imai M., Miyakawa Y., Mayumi M. 1988; Typing hepatitis B virus by homology in nucleotide sequence: comparison of surface antigen subtypes. J Gen Virol 69:2575–2583
     [Google Scholar]
  36. Okamoto H., Yotsumoto S., Akahane Y. 7 other authors 1990; Hepatitis B viruses with precore region defects prevail in persistently infected hosts along with seroconversion to the antibody against e antigen. J Virol 64:1298–1303
     [Google Scholar]
  37. Olweny C. L. 1984; Etiology of hepatocellular carcinoma in Africa. IARC Sci Publ 63:89–95
     [Google Scholar]
  38. Omata M., Ehata T., Yokosuka O., Hosoda K., Ohto M. 1991; Mutations in the precore region of hepatitis B virus DNA in patients with fulminant and severe hepatitis. N Engl J Med 324:1699–1704
     [Google Scholar]
  39. Orito E., Mizokami M., Sakugawa H., Michitaka K., Ishikawa K., Ichida T., Okanoue T., Yotsuyanagi H., Iino S. 2001; A case-control study for clinical and molecular biological differences between hepatitis B viruses of genotypes B and C. Japan HBV Genotype Research Group. Hepatology 33:218–223
     [Google Scholar]
  40. Owiredu W. K., Kramvis A., Kew M. C. 2001; Hepatitis B virus DNA in serum of healthy black African adults positive for hepatitis B surface antibody alone: possible association with recombination between genotypes A and D. J Med Virol 64:441–454
     [Google Scholar]
  41. Rieger A., Nassal M. 1995; Distinct requirements for primary sequence in the 5′- and 3′-part of a bulge in the hepatitis B virus RNA encapsidation signal revealed by a combined in vivo selection/in vitro amplification system. Nucleic Acids Res 23:3909–3915
     [Google Scholar]
  42. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
     [Google Scholar]
  43. Santantonio T., Jung M. C., Miska S., Pastore G., Pape G. R., Will H. 1991; Prevalence and type of pre-C HBV mutants in anti-HBe positive carriers with chronic liver disease in a highly endemic area. Virology 183:840–844
     [Google Scholar]
  44. Sato S., Suzuki K., Akahane Y. 8 other authors 1995; Hepatitis B virus strains with mutations in the core promoter in patients with fulminant hepatitis. Ann Intern Med 122:241–248
     [Google Scholar]
  45. Stuyver L., De Gendt S., Van Geyt C., Zoulim F., Fried M., Schinazi R. F., Rossau R. 2000; A new genotype of hepatitis B virus: complete genome and phylogenetic relatedness. J Gen Virol 81:67–74
     [Google Scholar]
  46. Sugauchi F., Mizokami M., Orito E. 7 other authors 2001; A novel variant genotype C of hepatitis B virus identified in isolates from Australian Aborigines: complete genome sequence and phylogenetic relatedness. J Gen Virol 82:883–892
     [Google Scholar]
  47. Sugauchi F., Chutaputti A., Orito E., Kato H., Suzuki S., Ueda R., Mizokami M. 2002a; Hepatitis B virus genotypes and clinical manifestation among hepatitis B carriers in Thailand. J Gastroenterol Hepatol 17:671–676
     [Google Scholar]
  48. Sugauchi F., Orito E., Ichida T. & 9 other authors (2002b). Hepatitis B virus of genotype B with or without recombination with genotype C over the precore region plus the core gene. J Virol 76:5985–5992
     [Google Scholar]
  49. Sugauchi F., Orito E., Kato H. 8 other authors 2003a; Genotype, serotype, and phylogenetic characterization of the complete genome sequence of hepatitis B virus isolates from Malawian chronic carriers of the virus. J Med Virol 69:33–40
     [Google Scholar]
  50. Sugauchi F., Orito E., Sakugawa H. 7 other authors 2003b; Epidemiologic and virologic characteristics of hepatitis B virus genotype B having the recombination with genotype C. Gastroenterology 124:925–932
     [Google Scholar]
  51. Takahashi K., Akahane Y., Hino K., Ohta Y., Mishiro S. 1998; Hepatitis B virus genomic sequence in the circulation of hepatocellular carcinoma patients: comparative analysis of 40 full-length isolates. Arch Virol 143:2313–2326
     [Google Scholar]
  52. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680
     [Google Scholar]
  53. Tran A., Kremsdorf D., Capel F., Housset C., Dauguet C., Petit M. A., Brechot C. 1991; Emergence of and takeover by hepatitis B virus (HBV) with rearrangements in the pre-S/S and pre-C/C genes during chronic HBV infection. J Virol 65:3566–3574
     [Google Scholar]
  54. Usuda S., Okamoto H., Iwanari H., Baba K., Tsuda F., Miyakawa Y., Mayumi M. 1999; Serological detection of hepatitis B virus genotypes by ELISA with monoclonal antibodies to type-specific epitopes in the preS2-region product. J Virol Methods 80:97–112
     [Google Scholar]
  55. Usuda S., Okamoto H., Tanaka T., Kidd-Ljunggren K., Holland P. V., Miyakawa Y., Mayumi M. 2000; Differentiation of hepatitis B virus genotypes D and E by ELISA using monoclonal antibodies to epitopes on the preS2-region product. J Virol Methods 87:81–89
     [Google Scholar]
  56. Valliammai T., Thyagarajan S. P., Zuckerman A. J., Harrison T. J. 1995; Precore and core mutations in HBV from individuals in India with chronic infection. J Med Virol 45:321–325
     [Google Scholar]
  57. Wai C. T., Chu C. J., Hussain M., Lok A. S. 2002; HBV genotype B is associated with better response to interferon therapy in HBeAg(+) chronic hepatitis than genotype C. Hepatology 36:1425–1430
     [Google Scholar]
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Epidemiological and sequence differences between two subtypes (Ae and Aa) of hepatitis B virus genotype A
J. Gen. Virol. 85, 811 (2004); https://doi.org/10.1099/vir.0.79811-0
/content/journal/jgv/10.1099/vir.0.79811-0
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