@article{mbs:/content/journal/jgv/10.1099/vir.0.79867-0, author = "Derrien, Muriel and Pizzato, Nathalie and Dolcini, Guillermina and Menu, Elisabeth and Chaouat, Gérard and Lenfant, Françoise and Barré-Sinoussi, Françoise and Bouteiller, Philippe Le", title = "Human immunodeficiency virus 1 downregulates cell surface expression of the non-classical major histocompatibility class I molecule HLA-G1", journal= "Journal of General Virology", year = "2004", volume = "85", number = "7", pages = "1945-1954", doi = "https://doi.org/10.1099/vir.0.79867-0", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.79867-0", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "Human immunodeficiency virus 1 (HIV-1) downregulates cell surface expression of HLA-A and HLA-B but not HLA-C or HLA-E to ultimately escape immune defences. Here, it is shown that cell surface expression of the non-classical HLA-G1 is also downregulated by HIV-1, by using co-transfection experiments and infection with cell-free HIV-1 of HLA-G1-expressing U87 glioma cells or macrophages in primary culture. Moreover, co-transfection experiments using proviruses deleted in either nef or vpu or plasmids encoding HIV-1 Nef and Vpu mixed together with a HLA-G1-expressing construct demonstrated that HLA-G1 downregulation is Nef-independent and Vpu-dependent, contrasting with the Nef- and Vpu-dependent HLA-A2 downregulation. Together, these results show that the decrease of HLA-A2 and HLA-G1 caused by HIV-1 occurs through distinct mechanisms.", }