Inhibition of influenza virus matrix (M1) protein expression and virus replication by U6 promoter-driven and lentivirus-mediated delivery of siRNA Hui, Eric Ka-Wai and Yap, Ee Ming and An, Dong Sung and Chen, Irvin S. Y. and Nayak, Debi P.,, 85, 1877-1884 (2004), doi = https://doi.org/10.1099/vir.0.79906-0, publicationName = Microbiology Society, issn = 0022-1317, abstract= Small interfering RNA (siRNA)-induced RNA degradation has been used recently as an antivirus agent to inhibit specific virus replication. This report shows that 21 nt duplexes of siRNA of the influenza virus M gene can cause specific inhibition of influenza virus matrix (M1) protein expression in transfected 293T cells. Furthermore, it is shown that a lentivirus vector can be used to effectively deliver M gene siRNAs into Madin–Darby canine kidney cells and can cause specific inhibition of M1 protein expression and influenza virus replication. Therefore, lentivirus-mediated delivery of siRNA and gene silencing can be used in studying the specific functions of virus genes in replication and may have a potential therapeutic application., language=, type=