1887

Abstract

This study reports on the complete genome sequence of , the type member of the genus . The genome is 17 919 nt in size and contains 13 open reading frames (ORFs). Previously, the sequence of 13 154 nt of the 3′-terminal of the genome was reported. The newly sequenced portion contains a 158 nt 5′ UTR, a single papain-like protease and a methyltransferase-like (MT) domain. ORF1a encodes a large polypeptide with a molecular mass of 245 kDa. With a predicted +1 frameshift, the large fusion protein generated from ORF1a/1b would produce a 306 kDa polypeptide. Phylogenetic analysis using MT domains further supports the creation of the genus for mealy-bug-transmitted viruses in the family .

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2004-07-01
2024-04-18
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References

  1. Agranovsky A. A., Koonin E. V., Boyko V. P., Maiss E., Frotschl R., Lunina N. A., Atabekov J. G. 1994; Beet yellows closterovirus: complete genome structure and identification of a leader papain-like thiol protease. Virology 198:311–324 [CrossRef]
    [Google Scholar]
  2. Dolja V. V., Karasev A. V., Koonin E. V. 1994; Molecular biology and evolution of closteroviruses: sophisticated build-up of large RNA genomes. Annu Rev Phytopathol 32:261–285 [CrossRef]
    [Google Scholar]
  3. Eastwell K. C., Bernardy M. G. 2001; Partial characterization of a closterovirus associated with apple mealybug-transmitted little cherry disease in North America. Phytopathology 91:268–273 [CrossRef]
    [Google Scholar]
  4. Hu J. S., Gonsalves D., Teliz D. 1990; Characterization of closterovirus-like particles associated with grapevine leafroll disease. J Phytopathol 128:1–14 [CrossRef]
    [Google Scholar]
  5. Jelkmann W., Fechtner B., Agranovsky A. A. 1997; Complete genome structure and phylogenetic analysis of little cherry virus, a mealybug transmissible closterovirus. J Gen Virol 78:2067–2071
    [Google Scholar]
  6. Karasev A. V. 2000; Genetic diversity and evolution of closteroviruses. Annu Rev Phytopathol 38:293–324 [CrossRef]
    [Google Scholar]
  7. Karasev A. V., Boyko V. P., Gowda S. 10 other authors 1995; Complete sequence of the citrus tristeza virus RNA genome. Virology 208:511–520 [CrossRef]
    [Google Scholar]
  8. Klaassen V. A., Boeshore M. L., Koonin E. V., Tian T., Falk B. W. 1995; Genome structure and phylogenetic analysis of lettuce infectious yellows virus, a whitefly-transmitted, bipartite closterovirus. Virology 208:99–110 [CrossRef]
    [Google Scholar]
  9. Ling K. S., Zhu H. Y., Alvizo H., Hu J. S., Drong R. F., Slightom J. L., Gonsalves D. 1997; The coat protein gene of grapevine leafroll associated closterovirus-3: cloning, nucleotide sequencing and expression in transgenic plants. Arch Virol 142:1101–1116 [CrossRef]
    [Google Scholar]
  10. Ling K. S., Zhu H. Y., Drong R. F., Slightom J. L., Gonsalves D. 1998; Nucleotide sequence of the 3′-terminal two-thirds of the grapevine leafroll-associated virus-3 genome reveals a typical monopartite closterovirus. J Gen Virol 79:1299–1307
    [Google Scholar]
  11. Martelli G. P., Agranovsky A. A., Bar-Joseph M. 13 other authors 2002; The family Closteroviridae revised. Arch Virol 147:2039–2044 [CrossRef]
    [Google Scholar]
  12. Melzer M. J., Karasev A. V., Sether D. M., Hu J. S. 2001; Nucleotide sequence, genome organization and phylogenetic analysis of pineapple mealybug wilt-associated virus-2. J Gen Virol 82:1–7
    [Google Scholar]
  13. Rott M. E., Jelkmann W. 2001; Detection and partial characterization of a second closterovirus associated with little cherry disease, little cherry virus-2. Phytopathology 91:261–267 [CrossRef]
    [Google Scholar]
  14. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual , 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  15. Zhu H.-Y., Ling K.-S., Goszczynski D. E., McFerson J. R., Gonsalves D. 1998; Nucleotide sequence and genome organization of grapevine leafroll-associated virus-2 is similar to beet yellows virus, the closterovirus type member. J Gen Virol 79:1289–1298
    [Google Scholar]
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