1887

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Volume 85, Issue 11

Inhibition of foot-and-mouth disease virus replication by small interfering RNA Free

Abstract

Foot-and-mouth disease, caused by foot-and-mouth disease virus (FMDV), is one of the most dangerous diseases of cloven-hoofed animals and is a constant threat to the dairy and beef industries in the Middle East and other regions of the world, despite intensive vaccination programmes. In this work, the ability of specific small interfering (si)RNAs to inhibit virus replication in BHK-21 cells was examined. By using bioinformatic computer programs, all FMDV sequences in public-domain databases were analysed. The analysis revealed three regions of at least 22 bp with 100 % identity in all FMDV entries. From these sequences, three specific siRNA molecules were prepared and used to test the ability of siRNAs to inhibit virus replication. By using real-time quantitative PCR to measure the amount of viral RNA in infected cells, it was shown that virus replication was inhibited in cells that were transfected with siRNAs. When viral titres were examined, 100 % inhibition of growth could be demonstrated in cells transfected with a mixture of all three anti-FMDV siRNAs, compared with control cells transfected with anti- siRNA.

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2004-11-01
2024-04-20
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References

  1. Bass B. L. 2000; Double-stranded RNA as a template for gene silencing. Cell 101:235–238 [CrossRef]
     [Google Scholar]
  2. Bernstein E., Caudy A. A., Hammond S. M., Hannon G. J. 2001; Role for a bidentate ribonuclease in the initiation step of RNA interference. Nature 409:363–366 [CrossRef]
     [Google Scholar]
  3. Capodici J., Karikó K., Weissman D. 2002; Inhibition of HIV-1 infection by small interfering RNA-mediated RNA interference. J Immunol 169:5196–5201 [CrossRef]
     [Google Scholar]
  4. Carroll A. R., Rowlands D. J., Clarke B. E. 1984; The complete nucleotide sequence of the RNA for the primary translation product of foot and mouth disease virus. Nucleic Acids Res 12:2461–2472 [CrossRef]
     [Google Scholar]
  5. Dopazo J., Sobrino F., Palma E. L., Domingo E., Moya A. 1988; Gene encoding capsid protein VP1 of foot-and-mouth disease virus: a quasispecies model of molecular evolution. Proc Natl Acad Sci U S A 85:6811–6815 [CrossRef]
     [Google Scholar]
  6. Elbashir S. M., Harborth J., Lendeckel W., Yalcin A., Weber K., Tuschl T. 2001; Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature 411:494–498 [CrossRef]
     [Google Scholar]
  7. Fire A., Xu S., Montgomery M. K., Kostas S. A., Driver S. E., Mello C. C. 1998; Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans . Nature 391:806–811 [CrossRef]
     [Google Scholar]
  8. Forss S., Strebel K., Beck E., Schaller H. 1984; Nucleotide sequence and genomic organization of foot-and-mouth disease virus. Nucleic Acids Res 12:6587–6601 [CrossRef]
     [Google Scholar]
  9. Giladi H., Ketzinel-Gilad M., Rivkin L., Felig Y., Nussbaum O., Galun E. 2003; Small interfering RNA inhibits hepatitis B virus replication in mice. Mol Ther 8:769–776 [CrossRef]
     [Google Scholar]
  10. Gitlin L., Karelsky S., Andino R. 2002; Short interfering RNA confers intracellular antiviral immunity in human cells. Nature 418:430–434 [CrossRef]
     [Google Scholar]
  11. Kasschau K. D., Carrington J. C. 1998; A counterdefensive strategy of plant viruses: suppression of posttranscriptional gene silencing. Cell 95:461–470 [CrossRef]
     [Google Scholar]
  12. Kennerdell J. R., Carthew R. W. 1998; Use of dsRNA-mediated genetic interference to demonstrate that frizzled and frizzled 2 act in the Wingless pathway. Cell 95:1017–1026 [CrossRef]
     [Google Scholar]
  13. Mason P. W., Grubman M. J., Baxt B. 2003; Molecular basis of pathogenesis of FMDV. Virus Res 91:9–32 [CrossRef]
     [Google Scholar]
  14. Montgomery M. K., Xu S., Fire A. 1998; RNA as a target of double-stranded RNA-mediated genetic interference in Caenorhabditis elegans . Proc Natl Acad Sci U S A 95:15502–15507 [CrossRef]
     [Google Scholar]
  15. Ngô H., Tschudi C., Gull K., Ullu E. 1998; Double-stranded RNA induces mRNA degradation in Trypanosoma brucei . Proc Natl Acad Sci U S A 95:14687–14692 [CrossRef]
     [Google Scholar]
  16. Park W.-S., Miyano-Kurosaki N., Hayafune M., Nakajima E., Matsuzaki T., Shimada F., Takaku H. 2002; Prevention of HIV-1 infection in human peripheral blood mononuclear cells by specific RNA interference. Nucleic Acids Res 30:4830–4835 [CrossRef]
     [Google Scholar]
  17. Pearson W. R., Lipman D. J. 1988; Improved tools for biological sequence comparison. Proc Natl Acad Sci U S A 85:2444–2448 [CrossRef]
     [Google Scholar]
  18. Samuel A. R., Knowles N. J. 2001; Foot-and-mouth disease virus: cause of the recent crisis for the UK livestock industry. Trends Genet 17:421–424 [CrossRef]
     [Google Scholar]
  19. Stram Y., Molad T. 1997; A ribozyme targeted to cleave the polymerase gene sequences of different foot-and-mouth disease virus (FMDV) serotypes. Virus Genes 15:33–37 [CrossRef]
     [Google Scholar]
  20. Stram Y., Chai D., Fawzy H.-E. 7 other authors 1995; Molecular epidemiology of foot-and-mouth disease (FMD) in Israel in 1994 and in other Middle-Eastern countries in the years 1992–1994. Arch Virol 140:1791–1797 [CrossRef]
     [Google Scholar]
  21. Sui G., Soohoo C., Affar E. B., Gay F., Shi Y., Forrester W. C., Shi Y. 2002; A DNA vector-based RNAi technology to suppress gene expression in mammalian cells. Proc Natl Acad Sci U S A 99:5515–5520 [CrossRef]
     [Google Scholar]
  22. Tabara H., Grishok A., Mello C. C. 1998; RNAi in C. elegans : soaking in the genome sequence. Science 282:430–431 [CrossRef]
     [Google Scholar]
  23. Takeda A., Sugiyama K., Nagano H., Mori M., Kaido M., Mise K., Tsuda S., Okuno T. 2002; Identification of a novel RNA silencing suppressor, NSs protein of Tomato spotted wilt virus . FEBS Lett 532:75–79 [CrossRef]
     [Google Scholar]
  24. Voinnet O. 2001; RNA silencing as a plant immune system against viruses. Trends Genet 17:449–459 [CrossRef]
     [Google Scholar]
  25. Voinnet O., Pinto Y. M., Baulcombe D. C. 1999; Suppression of gene silencing: a general strategy used by diverse DNA and RNA viruses of plants. Proc Natl Acad Sci U S A 96:14147–14152 [CrossRef]
     [Google Scholar]
  26. Waterhouse P. M., Graham M. W., Wang M. B. 1998; Virus resistance and gene silencing in plants can be induced by simultaneous expression of sense and antisense RNA. Proc Natl Acad Sci U S A 95:13959–13964 [CrossRef]
     [Google Scholar]
  27. Waterhouse P. M., Wang M. B., Lough T. 2001; Gene silencing as an adaptive defence against viruses. Nature 411:834–842 [CrossRef]
     [Google Scholar]
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Inhibition of foot-and-mouth disease virus replication by small interfering RNA
J. Gen. Virol. 85, 3213 (2004); https://doi.org/10.1099/vir.0.80133-0
/content/journal/jgv/10.1099/vir.0.80133-0
/content/journal/jgv/10.1099/vir.0.80133-0
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