%0 Journal Article %A Schirrmeier, Horst %A Strebelow, Günther %A Depner, Klaus %A Hoffmann, Bernd %A Beer, Martin %T Genetic and antigenic characterization of an atypical pestivirus isolate, a putative member of a novel pestivirus species %D 2004 %J Journal of General Virology, %V 85 %N 12 %P 3647-3652 %@ 1465-2099 %R https://doi.org/10.1099/vir.0.80238-0 %I Microbiology Society, %X The genus Pestivirus within the family Flaviviridae currently consists of four different main species: Classical swine fever virus, Bovine viral diarrhea virus types 1 and 2 and Border disease virus. A fifth tentative species is represented by an isolate from a giraffe. In this study, a completely new pestivirus, isolated from a batch of fetal calf serum that was collected in Brazil, is described. It is proposed that the isolate D32/00_‘HoBi’ may constitute a novel sixth pestivirus species, because it is genetically, as well as antigenically, markedly different from all other pestiviruses. Based on the entire Npro- and E2-encoding sequences, identities of <70 % to all other pestivirus species were determined. Similarly, cross-neutralization and binding studies using antisera and mAbs revealed marked antigenic differences between D32/00_‘HoBi’ and all other pestiviruses. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.80238-0