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Volume 85, Issue 11

Complete genome sequence of /Cp9B21, a member of a novel genus within the family , isolated from the chestnut blight fungus Free

Abstract

(MYRV-1) is the type species of the newly described genus of the large virus family . The virus was isolated from a hypovirulent strain (9B21) of the chestnut blight fungus, . A previous study showed that double-shelled particles introduced to fungal spheroplasts resulted in stably infected colonies. Of the 11 double-stranded RNA genomic segments (S1–S11), the three largest (S1–S3) were sequenced previously and shown to have moderate levels of similarity to the homologous segments of mammal-pathogenic coltiviruses ( and ) and another fungus-infecting reovirus, of strain W370 (MYRV-3/RnW370). The sequences of the remaining segments (S4–S11) are reported here. All of the segments have single ORFs on their positive strands and the terminal sequences 5′-GAUCA----GCAGUCA-3′ are conserved among currently and previously sequenced segments. Oligo-cap analysis showed that the positive strands of the genomic segments are capped, whereas the negative strands are not. Similarities among the four evolutionarily related viruses include low or moderate levels of amino acid sequence identity (14·7–34·2 %) and isoelectric points among equivalent polypeptides, e.g. proteins encoded by segments S4 and S5 of the four viruses. Phylogenetic analysis indicated that MYRV-1/Cp9B21 is related more closely to MYRV-3/RnW370 than to the coltiviruses. An interesting dissimilarity is found in codon-choice pattern among the four viruses, i.e. MYRV-1/Cp9B21 segments have a lower frequency of [XYG+XYC] than corresponding segments of the other viruses, suggesting a possible adjustment of virus codon usage to their host environments.

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2004-11-01
2024-04-19
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Complete genome sequence of Mycoreovirus-1/Cp9B21, a member of a novel genus within the family Reoviridae, isolated from the chestnut blight fungus Cryphonectria parasitica
J. Gen. Virol. 85, 3437 (2004); https://doi.org/10.1099/vir.0.80293-0
/content/journal/jgv/10.1099/vir.0.80293-0
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