@article{mbs:/content/journal/jgv/10.1099/vir.0.80426-0, author = "Suspène, Rodolphe and Henry, Michel and Guillot, Sophie and Wain-Hobson, Simon and Vartanian, Jean-Pierre", title = "Recovery of APOBEC3-edited human immunodeficiency virus G→A hypermutants by differential DNA denaturation PCR", journal= "Journal of General Virology", year = "2005", volume = "86", number = "1", pages = "125-129", doi = "https://doi.org/10.1099/vir.0.80426-0", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.80426-0", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "Virus genomes from the same family may exhibit a wide range in their DNA GC content, whereas viral hypermutants differ substantially in GC content from their parental genomes. As AT-rich DNA melts at lower temperatures than GC-rich DNA, use of a lower denaturation temperature during PCR should allow differential amplification of AT-rich genomes or variants within a quasispecies. The latter situation has been explored explicitly in a two-step process by using a series of well-defined viral sequences differing in their AT content. Firstly, the lowest denaturation temperature (T p) that allowed amplification of the parental sequence was determined. Secondly, differential amplification of AT-rich viral variants was obtained by using a denaturation temperature 1–3 °C lower than T p. Application of this sensitive method to two different viruses allowed us to identify human immunodeficiency virus type 1 G→A hypermutants in a situation where none were expected and to amplify AT-rich variants selectively within a spectrum of poliovirus mutants.", }