1887

Journal of General Virology header logo

Volume 85, Issue 12

Hepatitis C virus population analysis of a single-source nosocomial outbreak reveals an inverse correlation between viral load and quasispecies complexity Free

Abstract

The features of (HCV) quasispecies within an envelope segment including the hypervariable region 1 were analysed at an early time point post-infection in seven patients that acquired HCV from a single common donor during a nosocomial outbreak. The grouping of patients according to viral load was reflected in the structure of the quasispecies. A higher viral load correlated with the presence of a predominant HCV genome and a corresponding lower quasispecies complexity. The quasispecies complexity itself was not correlated with HCV clearance or persistence. Thus, the relationship between an intrapatient HCV quasispecies and the clinical outcome of an HCV infection is more complex than previously anticipated.

  • Received:
  • Accepted:
  • Published Online:
SGM
Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.80500-0
2004-12-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/85/12/vir853619.html?itemId=/content/journal/jgv/10.1099/vir.0.80500-0&mimeType=html&fmt=ahah

References

  1. Allander T., Beyene A., Jacobson S. H., Grillner L., Persson M. A. 1997; Patients infected with the same hepatitis C virus strain display different kinetics of the isolate-specific antibody response. J Infect Dis 175:26–31 [CrossRef]
     [Google Scholar]
  2. Alter M. J., Kruszon-Moran D., Nainan O. V., McQuillan G. M., Gao F., Moyer L. A., Kaslow R. A., Margolis H. S. 1999; The prevalence of hepatitis C virus infection in the United States, 1988 through 1994. N Engl J Med 341:556–562 [CrossRef]
     [Google Scholar]
  3. Bain C., Fatmi A., Zoulim F., Zarski J. P., Trepo C., Inchauspe G. 2001; Impaired allostimulatory function of dendritic cells in chronic hepatitis C infection. Gastroenterology 120:512–524 [CrossRef]
     [Google Scholar]
  4. Booth J. C., Kumar U., Webster D., Monjardino J., Thomas H. C. 1998; Comparison of the rate of sequence variation in the hypervariable region of E2/NS1 region of hepatitis C virus in normal and hypogammaglobulinemic patients. Hepatology 27:223–227 [CrossRef]
     [Google Scholar]
  5. Bruguera M., Saiz J. C., Franco S. 7 other authors 2002; Outbreak of nosocomial hepatitis C virus infection resolved by genetic analysis of HCV RNA. J Clin Microbiol 40:4363–4366 [CrossRef]
     [Google Scholar]
  6. Chang K. M., Rehermann B., McHutchison J. G., Pasquinelli C., Southwood S., Sette A., Chisari F. V. 1997; Immunological significance of cytotoxic T lymphocyte epitope variants in patients chronically infected by the hepatitis C virus. J Clin Invest 100:2376–2385 [CrossRef]
     [Google Scholar]
  7. Christie J. M., Chapel H., Chapman R. W., Rosenberg W. M. 1999; Immune selection and genetic sequence variation in core and envelope regions of hepatitis C virus. Hepatology 30:1037–1044 [CrossRef]
     [Google Scholar]
  8. Cooper S., Erickson A. L., Adams E. J., Kansopon J., Weiner A. J., Chien D. Y., Houghton M., Parham P., Walker C. M. 1999; Analysis of a successful immune response against hepatitis C virus. Immunity 10:439–449 [CrossRef]
     [Google Scholar]
  9. Domingo E., Mas A., Yuste E., Pariente N., Sierra S., Gutierrez-Riva M., Menendez-Arias L. 2001; Virus population dynamics, fitness variations and the control of viral disease: an update. Prog Drug Res 57:77–115
     [Google Scholar]
  10. Eigen M., Biebricher C. K. 1988; Sequence space and quasispecies distribution. In RNA Genetics pp  211–245 Edited by Domingo E., Holland J. J., Ahlquist P. Boca Raton, Florida: CRC Press Inc;
     [Google Scholar]
  11. Farci P., Purcell R. H. 2000; Clinical significance of hepatitis C virus genotypes and quasispecies. Semin Liver Dis 20:103–126
     [Google Scholar]
  12. Farci P., Shimoda A., Wong D. 7 other authors 1996; Prevention of hepatitis C virus infection in chimpanzees by hyperimmune serum against the hypervariable region 1 of the envelope 2 protein. Proc Natl Acad Sci U S A 93:15394–15399 [CrossRef]
     [Google Scholar]
  13. Farci P., Shimoda A., Coiana A. 9 other authors 2000; The outcome of acute hepatitis C predicted by the evolution of the viral quasispecies. Science 288:339–344 [CrossRef]
     [Google Scholar]
  14. Gimenez-Barcons M., Franco S., Suarez Y. 10 other authors 2001; High amino acid variability within the NS5A of hepatitis C virus (HCV) is associated with hepatocellular carcinoma in patients with HCV-1b-related cirrhosis. Hepatology 34:158–167 [CrossRef]
     [Google Scholar]
  15. Hsu M., Zhang J., Flint M., Logvinoff C., Cheng-Mayer C., Rice C. M., McKeating J. A. 2003; Hepatitis C virus glycoproteins mediate pH-dependent cell entry of pseudotyped retroviral particles. Proc Natl Acad Sci U S A 100:7271–7276 [CrossRef]
     [Google Scholar]
  16. Kao J. H., Chen P. J., Lai M. Y., Wang T. H., Chen D. S. 1995; Quasispecies of hepatitis C virus and genetic drift of the hypervariable region in chronic type C hepatitis. J Infect Dis 172:261–264 [CrossRef]
     [Google Scholar]
  17. Kato N., Sekiya H., Ootsuyama Y., Nakazawa T., Hijikata M., Ohkoshi S., Shimotohno K. 1993; Humoral immune response to hypervariable region 1 of the putative envelope glycoprotein (gp70) of hepatitis C virus. J Virol 67:3923–3930
     [Google Scholar]
  18. Kato N., Ootsuyama Y., Sekiya H., Ohkoshi S., Nakazawa T., Hijikata M., Shimotohno K. 1994; Genetic drift in hypervariable region 1 of the viral genome in persistent hepatitis C virus infection. J Virol 68:4776–4784
     [Google Scholar]
  19. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [CrossRef]
     [Google Scholar]
  20. Kumar S., Tamura K., Jakobsen I. B., Nei M. 2001; mega2: Molecular evolutionary genetics analysis software. Bioinformatics 17:1244–1245 [CrossRef]
     [Google Scholar]
  21. Logvinoff C., Major M. E., Oldach D. 7 other authors 2004; Neutralizing antibody response during acute and chronic hepatitis C virus infection. Proc Natl Acad Sci U S A 101:10149–10154 [CrossRef]
     [Google Scholar]
  22. Major M. E., Dahari H., Mihalik K., Puig M., Rice C. M., Neumann A. U., Feinstone S. M. 2004; Hepatitis C virus kinetics and host responses associated with disease and outcome of infection in chimpanzees. Hepatology 39:1709–1720 [CrossRef]
     [Google Scholar]
  23. Manzin A., Solforosi L., Petrelli E., Macarri G., Tosone G., Piazza M., Clementi M. 1998; Evolution of hypervariable region 1 of hepatitis C virus in primary infection. J Virol 72:6271–6276
     [Google Scholar]
  24. Manzin A., Solforosi L., Debiaggi M., Zara F., Tanzi E., Romano L., Zanetti A. R., Clementi M. 2000; Dominant role of host selective pressure in driving hepatitis C virus evolution in perinatal infection. J Virol 74:4327–4334 [CrossRef]
     [Google Scholar]
  25. Martell M., Esteban J. I., Quer J., Genesca J., Weiner A., Esteban R., Guardia J., Gomez J. 1992; Hepatitis C virus (HCV) circulates as a population of different but closely related genomes: quasispecies nature of HCV genome distribution. J Virol 66:3225–3229
     [Google Scholar]
  26. Matsuura Y., Tani H., Suzuki K. 8 other authors 2001; Characterization of pseudotype VSV possessing HCV envelope proteins. Virology 286:263–275 [CrossRef]
     [Google Scholar]
  27. Mellor J., Holmes E. C., Jarvis L. M., Yap P. L., Simmonds P. The International HCV Collaborative Study Group 1995; Investigation of the pattern of hepatitis C virus sequence diversity in different geographical regions: implications for virus classification. J Gen Virol 76:2493–2507 [CrossRef]
     [Google Scholar]
  28. Pawlotsky J. M. 2003; Hepatitis C virus genetic variability: pathogenic and clinical implications. Clin Liver Dis 7:45–66 [CrossRef]
     [Google Scholar]
  29. Racanelli V., Rehermann B. 2003; Hepatitis C virus infection: when silence is deception. Trends Immunol 24:456–464 [CrossRef]
     [Google Scholar]
  30. Ray S. C., Wang Y. M., Laeyendecker O., Ticehurst J. R., Villano S. A., Thomas D. L. 1999; Acute hepatitis C virus structural gene sequences as predictors of persistent viremia: hypervariable region 1 as a decoy. J Virol 73:2938–2946
     [Google Scholar]
  31. Shimizu Y. K., Hijikata M., Iwamoto A., Alter H. J., Purcell R. H., Yoshikura H. 1994; Neutralizing antibodies against hepatitis C virus and the emergence of neutralization escape mutant viruses. J Virol 68:1494–1500
     [Google Scholar]
  32. Simmonds P. 1995; Variability of hepatitis C virus. Hepatology 21:570–583 [CrossRef]
     [Google Scholar]
  33. Sung V. M., Shimodaira S., Doughty A. L., Picchio G. R., Can H., Yen T. S., Lindsay K. L., Levine A. M., Lai M. M. 2003; Establishment of B-cell lymphoma cell lines persistently infected with hepatitis C virus in vivo and in vitro: the apoptotic effects of virus infection. J Virol 77:2134–2146 [CrossRef]
     [Google Scholar]
  34. Thimme R., Bukh J., Spangenberg H. C., Wieland S., Pemberton J., Steiger C., Govindarajan S., Purcell R. H., Chisari F. V. 2002; Viral and immunological determinants of hepatitis C virus clearance, persistence, and disease. Proc Natl Acad Sci U S A 99:15661–15668 [CrossRef]
     [Google Scholar]
  35. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
     [Google Scholar]
  36. Tsai S. L., Chen Y. M., Chen M. H., Huang C. Y., Sheen I. S., Yeh C. T., Huang J. H., Kuo G. C., Liaw Y. F. 1998; Hepatitis C virus variants circumventing cytotoxic T lymphocyte activity as a mechanism of chronicity. Gastroenterology 115:954–965 [CrossRef]
     [Google Scholar]
  37. van Doorn L. J., Capriles I., Maertens G., DeLeys R., Murray K., Kos T., Schellekens H., Quint W. 1995; Sequence evolution of the hypervariable region in the putative envelope region E2/NS1 of hepatitis C virus is correlated with specific humoral immune responses. J Virol 69:773–778
     [Google Scholar]
  38. Volkenstein M. V. 1994 Physical approaches to biological evolution Berlin, Germany: Springer-Verlag;
     [Google Scholar]
  39. Wang Y. M., Ray S. C., Laeyendecker O., Ticehurst J. R., Thomas D. L. 1998; Assessment of hepatitis C virus sequence complexity by electrophoretic mobilities of both single- and double-stranded DNAs. J Clin Microbiol 36:2982–2989
     [Google Scholar]
  40. Weiner A. J., Geysen H. M., Christopherson C. 12 other authors 1992; Evidence for immune selection of hepatitis C virus (HCV) putative envelope glycoprotein variants: potential role in chronic HCV infections. Proc Natl Acad Sci U S A 89:3468–3472 [CrossRef]
     [Google Scholar]
  41. Weiner A., Erickson A. L., Kansopon J., Crawford K., Muchmore E., Hughes A. L., Houghton M., Walker C. M. 1995; Persistent hepatitis C virus infection in a chimpanzee is associated with emergence of a cytotoxic T lymphocyte escape variant. Proc Natl Acad Sci U S A 92:2755–2759 [CrossRef]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.80500-0
Loading
Hepatitis C virus population analysis of a single-source nosocomial outbreak reveals an inverse correlation between viral load and quasispecies complexity
J. Gen. Virol. 85, 3619 (2004); https://doi.org/10.1099/vir.0.80500-0
/content/journal/jgv/10.1099/vir.0.80500-0
/content/journal/jgv/10.1099/vir.0.80500-0
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error