@article{mbs:/content/journal/jgv/10.1099/vir.0.80530-0, author = "Zakhartchouk, Alexander N. and Viswanathan, Sathiyanarayanan and Mahony, James B. and Gauldie, Jack and Babiuk, Lorne A.", title = "Severe acute respiratory syndrome coronavirus nucleocapsid protein expressed by an adenovirus vector is phosphorylated and immunogenic in mice", journal= "Journal of General Virology", year = "2005", volume = "86", number = "1", pages = "211-215", doi = "https://doi.org/10.1099/vir.0.80530-0", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.80530-0", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "Severe acute respiratory syndrome coronavirus (SARS-CoV) has been identified as the aetiological agent of SARS. Thus, vaccination against SARS-CoV may represent an effective approach towards controlling SARS. The nucleocapsid (N) protein is thought to play a role in induction of cell-mediated immunity to SARS-CoV and thus it is important to characterize this protein. In the present study, an E1/partially E3-deleted, replication-defective human adenovirus 5 (Ad5) vector (Ad5-N-V) expressing the SARS-CoV N protein was constructed. The N protein, expressed in vitro by Ad5-N-V, was of the expected molecular mass of 50 kDa and was phosphorylated. Vaccination of C57BL/6 mice with Ad5-N-V generated potent SARS-CoV-specific humoral and T cell-mediated immune responses. These results show that Ad5-N-V may potentially be used as a SARS-CoV vaccine.", }