1887

Abstract

Borna disease virus (BDV) can persistently infect the central nervous system and induce CD8 T-cell-mediated neurological disease in MRL mice. To determine whether specific immune priming would prevent disease, a prime–boost immunization protocol was established in which intramuscular injection of a recombinant parapoxvirus expressing BDV nucleoprotein (BDV-N) was followed by intraperitoneal infection with vaccinia virus expressing BDV-N. Immunized wild-type and perforin-deficient mice remained healthy after intracerebral infection with BDV and contained almost no virus in the brain at 5 weeks post-challenge. Immunization failed to induce resistance against BDV in mice lacking mature CD8 T cells. Immunization of perforin-deficient mice with a poxvirus vector expressing mutant BDV-N lacking the known CD8 T-cell epitope did not efficiently block multiplication of BDV in the brain and did not prevent neurological disease, indicating that vaccine-induced immunity to BDV in wild-type and perforin-deficient mice resulted from the action of CD8 T cells.

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2005-02-01
2024-03-29
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References

  1. Bilzer T., Stitz L. 1994; Immune-mediated brain atrophy. CD8+ T cells contribute to tissue destruction during Borna disease. J Immunol 153:818–823
    [Google Scholar]
  2. Bilzer T., Planz O., Lipkin W. I., Stitz L. 1995; Presence of CD4+ and CD8+ T cells and expression of MHC class I and MHC class II antigen in horses with Borna disease virus-induced encephalitis. Brain Pathol 5:223–230 [CrossRef]
    [Google Scholar]
  3. Binder G. K., Griffin D. E. 2001; Interferon- γ -mediated site-specific clearance of alphavirus from CNS neurons. Science 293:303–306 [CrossRef]
    [Google Scholar]
  4. Briese T., Schneemann A., Lewis A. J., Park Y. S., Kim S., Ludwig H., Lipkin W. I. 1994; Genomic organization of Borna disease virus. Proc Natl Acad Sci U S A 91:4362–4366 [CrossRef]
    [Google Scholar]
  5. Cubitt B., Oldstone C., de la Torre J. C. 1994; Sequence and genome organization of Borna disease virus. J Virol 68:1382–1396
    [Google Scholar]
  6. Fassnacht U., Ackermann A., Staeheli P., Hausmann J. 2004; Immunization with dendritic cells can break immunological ignorance toward a persisting virus in the central nervous system and induce partial protection against intracerebral viral challenge. J Gen Virol 85:2379–2387 [CrossRef]
    [Google Scholar]
  7. Fischer T., Planz O., Stitz L., Rziha H. J. 2003; Novel recombinant parapoxvirus vectors induce protective humoral and cellular immunity against lethal herpesvirus challenge infection in mice. J Virol 77:9312–9323 [CrossRef]
    [Google Scholar]
  8. Freude S., Hausmann J., Hofer M., Pham-Mitchell N., Campbell I. L., Staeheli P., Pagenstecher A. 2002; Borna disease virus accelerates inflammation and disease associated with transgenic expression of interleukin-12 in the central nervous system. J Virol 76:12223–12232 [CrossRef]
    [Google Scholar]
  9. Friedl G., Hofer M., Auber B., Sauder C., Hausmann J., Staeheli P., Pagenstecher A. 2004; Borna disease virus multiplication in mouse organotypic slice cultures is site-specifically inhibited by gamma interferon but not by interleukin-12. J Virol 78:1212–1218 [CrossRef]
    [Google Scholar]
  10. Gonzalez-Dunia D., Sauder C., de la Torre J. C. 1997; Borna disease virus and the brain. Brain Res Bull 44:647–664 [CrossRef]
    [Google Scholar]
  11. Guidotti L. G. 2002; The role of cytotoxic T cells and cytokines in the control of hepatitis B virus infection. Vaccine 20 (Suppl. 4):A80–A82 [CrossRef]
    [Google Scholar]
  12. Hallensleben W., Schwemmle M., Hausmann J., Stitz L., Volk B., Pagenstecher A., Staeheli P. 1998; Borna disease virus-induced neurological disorder in mice: infection of neonates results in immunopathology. J Virol 72:4379–4386
    [Google Scholar]
  13. Hausmann J., Hallensleben W., de la Torre J. C., Pagenstecher A., Zimmermann C., Pircher H., Staeheli P. 1999; T cell ignorance in mice to Borna disease virus can be overcome by peripheral expression of the viral nucleoprotein. Proc Natl Acad Sci U S A 96:9769–9774 [CrossRef]
    [Google Scholar]
  14. Hausmann J., Schamel K., Staeheli P. 2001; CD8+ T lymphocytes mediate Borna disease virus-induced immunopathology independently of perforin. J Virol 75:10460–10466 [CrossRef]
    [Google Scholar]
  15. Henkel M., Planz O., Fischer T., Stitz L., Rziha H. J. 2005; Prevention of virus persistence and protection against immunopathology after Borna disease virus infection of the brain by a novel Orf virus recombinant. J Virol 79: (in press
    [Google Scholar]
  16. Kagi D., Hengartner H. 1996; Different roles for cytotoxic T cells in the control of infections with cytopathic versus noncytopathic viruses. Curr Opin Immunol 8:472–477 [CrossRef]
    [Google Scholar]
  17. Lewis A. J., Whitton J. L., Hatalski C. G., Weissenbock H., Lipkin W. I. 1999; Effect of immune priming on Borna disease. J Virol 73:2541–2546
    [Google Scholar]
  18. Lin M. T., Stohlman S. A., Hinton D. R. 1997; Mouse hepatitis virus is cleared from the central nervous systems of mice lacking perforin-mediated cytolysis. J Virol 71:383–391
    [Google Scholar]
  19. Nöske K., Bilzer T., Planz O., Stitz L. 1998; Virus-specific CD4+ T cells eliminate Borna disease virus from the brain via induction of cytotoxic CD8+ T cells. J Virol 72:4387–4395
    [Google Scholar]
  20. Pena-Rossi C., McAllister A., Tanguy M., Kagi D., Brahic M. 1998; Theiler's virus infection of perforin-deficient mice. J Virol 72:4515–4519
    [Google Scholar]
  21. Planz O., Dumrese T., Hulpusch S., Schirle M., Stevanovic S., Stitz L. 2001; A naturally processed rat major histocompatibility complex class I-associated viral peptide as target structure of Borna disease virus-specific CD8+ T cells. J Biol Chem 276:13689–13694
    [Google Scholar]
  22. Schamel K., Staeheli P., Hausmann J. 2001; Identification of the immunodominant H-2Kk-restricted cytotoxic T-cell epitope in the Borna disease virus nucleoprotein. J Virol 75:8579–8588 [CrossRef]
    [Google Scholar]
  23. Sobbe M., Bilzer T., Gommel S., Nöske K., Planz O., Stitz L. 1997; Induction of degenerative brain lesions after adoptive transfer of brain lymphocytes from Borna disease virus-infected rats: presence of CD8+ T cells and perforin mRNA. J Virol 71:2400–2407
    [Google Scholar]
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