RT Journal Article SR Electronic(1) A1 Koshizuka, Tetsuo A1 Kawaguchi, Yasushi A1 Nishiyama, YukihiroYR 2005 T1 Herpes simplex virus type 2 membrane protein UL56 associates with the kinesin motor protein KIF1A JF Journal of General Virology, VO 86 IS 3 SP 527 OP 533 DO https://doi.org/10.1099/vir.0.80633-0 PB Microbiology Society, SN 1465-2099, AB The herpes simplex virus UL56 gene product is a C-terminal-anchored, type II membrane protein of unknown function. UL56 was found to interact with KIF1A, a member of the kinesin-3 family, in a yeast two-hybrid screen and a GST pull-down assay. KIF1A mediates the transport of synaptic vesicle precursors and is essential for the function and viability of neurons. When overexpressed, KIF1A co-localized with full-sized UL56, but no clear co-localization was observed when co-expressed with the UL56 mutant protein lacking its C-terminal transmembrane domain (TMD). Although the C-terminal TMD was not essential for the interaction with KIF1A in the yeast two-hybrid screen and GST pull-down assays, these results indicate that the C-terminal TMD, as well as aa 69–217, of UL56 are important for the interaction with KIF1A in vivo. The hypothesis that the UL56 protein affects vesicular trafficking in infected cells, potentially by acting as a receptor for motor proteins in neurons, is discussed., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.80633-0