RT Journal Article SR Electronic(1) A1 May, Janet S. A1 Coleman, Heather M. A1 Boname, Jessica M. A1 Stevenson, Philip G.YR 2005 T1 Murine gammaherpesvirus-68 ORF28 encodes a non-essential virion glycoprotein JF Journal of General Virology, VO 86 IS 4 SP 919 OP 928 DO https://doi.org/10.1099/vir.0.80661-0 PB Microbiology Society, SN 1465-2099, AB Murine gammaherpesvirus-68 (MHV-68) ORF28 is a gammaherpesvirus-specific gene of unknown function. Analysis of epitope-tagged ORF28 protein indicated that it was membrane-associated and incorporated into virions in N-glycosylated, O-glycosylated and unglycosylated forms. The extensive glycosylation of the small ORF28 extracellular domain – most forms of the protein appeared to be mainly carbohydrate by weight – suggested that a major function of ORF28 is to attach a variety of glycans to the virion surface. MHV-68 lacking ORF28 showed normal lytic replication in vitro and in vivo and normal latency establishment. MHV-68 ORF28 therefore encodes a small, membrane-bound and extensively glycosylated virion protein, whose function is entirely dispensable for normal, single-cycle host colonization., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.80661-0