1887

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Volume 86, Issue 6

Characteristics of the promoters derived from the single-stranded DNA components of in transgenic tobacco Free

Abstract

Predicted promoter regions of (MDV) components (C1–C11) were isolated and fused with a -glucuronidase (GUS) reporter gene and the characteristics of the promoters were examined. In transgenic tobacco calli, promoters of MDV C4 (encoding a cell-cycle link protein), C5 and C7 (both encoding unknown proteins), C6 (encoding a nuclear-shuttle protein) and C8 (encoding a movement protein) generated a stronger level of GUS expression than the 35S RNA promoter (). In leaves of transgenic tobacco plants, the promoters of C5 and C8 conferred a level of GUS activity comparable to that of . Histochemical GUS analysis showed that the promoters of C4–C9, the latter encoding a capsid protein, were active in phloem and meristematic tissue. The promoter of C8 was also active in mesophyll and cortex cell types. A low level of activity was found for the promoters of C11, which encodes a master replication-initiator protein (Rep), and C1, C2, C3 and C10, which encode additional Reps, in both transgenic tobacco calli and plants.

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2005-06-01
2024-04-18
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References

  1. Baumann K., De Paolis A., Constantino P., Gualberti G. 1999; The DNA binding site of the Dof protein NtBBF1 is essential for tissue-specific and auxin-regulated expression of the rolB oncogene in plants. Plant Cell 11:323–333 [CrossRef]
     [Google Scholar]
  2. Benfey P. N., Chua N.-H. 1990; The cauliflower mosaic virus 35S promoter: combinatorial regulation of transcription in plants. Science 250:959–966 [CrossRef]
     [Google Scholar]
  3. Bolle C., Sopory S., Lübberstedt T., Herrmann R. G., Oelmüller R. 1994; Segments encoding 5′-untranslated leaders of genes for thylakoid proteins contain cis -elements essential for transcription. Plant J 6:513–523 [CrossRef]
     [Google Scholar]
  4. Cheong Y. H., Yoo C. M., Park J. M., Ryu G. R., Goekjian V. H., Nagao R. T., Key J. L., Cho M. J., Hong J. C. 1998; STF1 is a novel TGACG-binding factor with a zinc-finger motif and a bZIP domain which heterodimerizes with GBF proteins. Plant J 15:199–209 [CrossRef]
     [Google Scholar]
  5. Chu P. W. G., Helms K. 1988; Novel virus-like particles containing circular single-stranded DNAs associated with subterranean clover stunt disease. Virology 167:38–49 [CrossRef]
     [Google Scholar]
  6. Chu P. W. G., Boevink P., Surin B., Larkin P., Keese P., Waterhouse P. 1995; Non geminated single-stranded DNA plant viruses. In Pathogenesis and Host Specificity in Plant Diseases vol III pp  311–341 Edited by Singh R. P., Singh U. S., Kohmoto K. Oxford: Pergamon Press;
     [Google Scholar]
  7. Dugdale B., Beetham P. R., Becker D. K., Harding R. M., Dale J. L. 1998; Promoter activity associated with the intergenic regions of banana bunchy top virus DNA-1 to -6 in transgenic tobacco and banana cells. J Gen Virol 79:2301–2311
     [Google Scholar]
  8. Dugdale B., Becker D. K., Beetham P. R., Harding R. M., Dale J. L. 2000; Promoters derived from banana bunchy top virus DNA-1 to -5 direct vascular-associated expression in transgenic banana ( Musa spp.). Plant Cell Rep 19:810–814 [CrossRef]
     [Google Scholar]
  9. Gronenborn B. 2004; Nanoviruses: genome organisation and protein function. Vet Microbiol 98:103–109 [CrossRef]
     [Google Scholar]
  10. Hanley-Bowdoin L., Settlage S. B., Orozco B. M., Nagar S., Robertson D. 2000; Geminiviruses: models for plant DNA replication, transcription, and cell cycle regulation. Crit Rev Biochem Mol Biol 35:105–140
     [Google Scholar]
  11. Harding R. M., Burns T. M., Dale J. L. 1991; Virus-like particles associated with banana bunchy top disease contain small single-stranded DNA. J Gen Virol 72:225–230 [CrossRef]
     [Google Scholar]
  12. Hehn A., Rohde W. 1998; Characterization of cis -acting elements affecting strength and phloem specificity of the coconut foliar decay virus promoter. J Gen Virol 79:1495–1499
     [Google Scholar]
  13. Higo K., Ugawa Y., Iwamoto M., Korenaga T. 1999; Plant cis -acting regulatory DNA elements (place) database: 1999. Nucleic Acids Res 27:297–300 [CrossRef]
     [Google Scholar]
  14. Hobbs S. L. A., Kpodar P., DeLong C. M. O. 1990; The effect of T-DNA copy number, position and methylation on reporter gene expression in tobacco transformants. Plant Mol Biol 15:851–864 [CrossRef]
     [Google Scholar]
  15. Hobbs S. L. A., Warkentin T. D., DeLong C. M. O. 1993; Transgene copy number can be positively or negatively associated with transgene expression. Plant Mol Biol 21:17–26 [CrossRef]
     [Google Scholar]
  16. Hoekema A., Hirsch P. R., Hooykaas P. J. J., Schilperoort R. A. 1983; A binary plant vector strategy based on separation of vir- and T-region of the Agrobacterium tumefaciens Ti-plasmid. Nature 303:179–180 [CrossRef]
     [Google Scholar]
  17. Horsch R. B., Fry J. E., Hoffman N. L., Eichholtz D., Rogers S. G., Fraley R. T. 1985; A simple and general method for transferring genes into plants. Science 227:1229–1231 [CrossRef]
     [Google Scholar]
  18. Horser C. L., Harding R. M., Dale J. L. 2001; Banana bunchy top nanovirus DNA-1 encodes the ‘master’ replication initiation protein. J Gen Virol 82:459–464
     [Google Scholar]
  19. Inouye T., Inouye N., Mitsuhata K. 1968; Yellow dwarf of pea and broad bean caused by milk vetch dwarf virus. Ann Phytopathol Soc Jpn 34:28–35 (in Japanese [CrossRef]
     [Google Scholar]
  20. Katul L., Vetten H. J., Maiss E., Makkouk K. M., Lesemann D.-E., Casper R. 1993; Characterisation and serology of virus-like particles associated with faba bean necrotic yellows. Ann Appl Biol 123:629–647 [CrossRef]
     [Google Scholar]
  21. Kosugi S., Ohashi Y., Nakajima K., Arai Y. 1990; An improved assay for β -glucuronidase in transformed cells: methanol almost completely suppresses a putative endogenous β -glucuronidase activity. Plant Sci 70:133–140 [CrossRef]
     [Google Scholar]
  22. Lam E., Benfey P. N., Gilmartin P. M., Fang R.-X., Chua N.-H. 1989; Site-specific mutations alter in vitro factor binding and change promoter expression pattern in transgenic plants. Proc Natl Acad Sci U S A 86:7890–7894 [CrossRef]
     [Google Scholar]
  23. Mikami K., Tabata T., Kawata T., Nakayama T., Iwabuchi M. 1987; Nuclear protein(s) binding to the conserved DNA hexameric sequence postulated to regulate transcription of wheat histone genes. FEBS Lett 223:273–278 [CrossRef]
     [Google Scholar]
  24. Morozov S. Ya., Merits A., Chernov B. K. 1994; Computer search of transcription control sequences in small plant virus DNA reveals a sequence highly homologous to the enhancer element of histone promoters. DNA Seq 4:395–397
     [Google Scholar]
  25. Murashige T., Skoog F. 1962; A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–497 [CrossRef]
     [Google Scholar]
  26. Nakayama T., Sakamoto A., Yang P., Minami M., Fujimoto Y., Ito T., Iwabuchi M. 1992; Highly conserved hexamer, octamer and nonamer motifs are positive cis -regulatory elements of the wheat histone H3 gene. FEBS Lett 300:167–170 [CrossRef]
     [Google Scholar]
  27. Ohki S. T., Doi Y., Yora K. 1975; Small spherical virus particles found in broad bean plants infected with milk vetch dwarf virus. Ann Phytopathol Soc Jpn 41:508–510 (in Japanese [CrossRef]
     [Google Scholar]
  28. Ohshima M., Itoh H., Matsuoka M., Murakami T., Ohashi Y. 1990; Analysis of stress-induced or salicylic acid-induced expression of the pathogenesis-related 1a protein gene in transgenic tobacco. Plant Cell 2:95–106 [CrossRef]
     [Google Scholar]
  29. Ooms G., Hooykaas P. J., Van Veen R. J., Van Beelen P., Regensburg-Tuink T. J., Schilperoort R. A. 1982; Octopine Ti-plasmid deletion mutants of Agrobacterium tumefaciens with emphasis on the right side of the T-region. Plasmid 7:15–29 [CrossRef]
     [Google Scholar]
  30. Peach C., Velten J. 1991; Transgene expression variability (position effect) of CAT and GUS reporter genes driven by linked divergent T-DNA promoters. Plant Mol Biol 17:49–60 [CrossRef]
     [Google Scholar]
  31. Randles J. W., Hanold D. 1989; Coconut foliar decay virus particles are 20-nm icosahedra. Intervirology 30:177–180
     [Google Scholar]
  32. Rohde W., Becker D., Randles J. W. 1995; The promoter of coconut foliar decay-associated circular single-stranded DNA directs phloem-specific reporter gene expression in transgenic tobacco. Plant Mol Biol 27:623–628 [CrossRef]
     [Google Scholar]
  33. Sano Y., Wada M., Hashimoto Y., Matsumoto T., Kojima M. 1998; Sequences of ten circular ssDNA components associated with the milk vetch dwarf virus genome. J Gen Virol 79:3111–3118
     [Google Scholar]
  34. Shirasawa-Seo N., Sano Y., Nakamura S. 8 other authors 2005; The promoter of Milk vetch dwarf virus component 8 confers effective gene expression in both dicot and monocot plants. Plant Cell Rep (in press
    [Google Scholar]
  35. Sunter G., Bisaro D. M. 1991; Transactivation in a geminivirus: AL2 gene product is needed for coat protein expression. Virology 180:416–419 [CrossRef]
     [Google Scholar]
  36. Surin B., Boevink P., Keese P., Chu P., Larkin P., Llewellyn D., Kahn R., Ellacott G., Waterhouse P. 1998; A suite of promoters and terminators for plant biotechnology. In Proceedings of the Fourth Asia-Pacific Conference on Agricultural Bio/Technology , abstract pp. 121–122 Canberra: UTC Publishing;
     [Google Scholar]
  37. Timchenko T., Katul L., Sano Y., de Kouchkovsky F., Vetten H. J., Gronenborn B. 2000; The master Rep concept in nanovirus replication: identification of missing genome components and potential for natural genetic reassortment. Virology 274:189–195 [CrossRef]
     [Google Scholar]
  38. Vetten H. J., Chu P. W. G., Dale J. L. 7 other authors; 2004; Nanoviridae . In Virus Taxonomy: Eighth Report of the International Committee on Taxonomy of Viruses pp  343–352 Edited by Fauquet C. M., Mayo M. A., Maniloff J., Desselberger U., Ball L. A. London: Elsevier/Academic Press;
     [Google Scholar]
  39. Yanagisawa S. 2002; The Dof family of plant transcription factors. Trends Plant Sci 7:555–560 [CrossRef]
     [Google Scholar]
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Characteristics of the promoters derived from the single-stranded DNA components of Milk vetch dwarf virus in transgenic tobacco
J. Gen. Virol. 86, 1851 (2005); https://doi.org/10.1099/vir.0.80790-0
/content/journal/jgv/10.1099/vir.0.80790-0
/content/journal/jgv/10.1099/vir.0.80790-0
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