1887

Abstract

Chloramphenicol acetyltransferase (CAT)-expressing negative-sense mini-genomic constructs of measles virus (MV) and rinderpest virus (RPV) were rescued by standard technology with helper plasmids expressing the nucleocapsid (N), phospho- (P) and large (L) proteins of MV, canine distemper virus (CDV) or RPV in order to determine whether the proteins of different viruses can function together. Homogeneous sets consisting of N, P and L plasmids derived from one virus were able to generate reporter gene expression from either mini-genomic construct. Heterogeneous sets of proteins from different viruses were not functional, with the exception that a low level of activity was obtained when MV N and P protein were combined with RPV L protein in the rescue of the MV mini-genomic construct, or CDV N was combined with RPV P and L in the rescue of the RPV mini-genome. However, only homogeneous sets of plasmids were able to rescue infectious virus from full-length anti-genome-expressing plasmids.

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2005-04-01
2024-04-19
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References

  1. Bankamp B., Kearney S. P., Liu X., Bellini W. J., Rota P. A. 2002; Activity of polymerase proteins of vaccine and wild-type measles virus strains in a minigenome replication assay. J Virol 76:7073–7081 [CrossRef]
    [Google Scholar]
  2. Bailey D., Banyard A., Dash P., Ozkul A., Barrett T. 2005; Full genome sequence of Peste des Petits Ruminants virus, a member of the Morbillivirus genus. Virus Res (in press
    [Google Scholar]
  3. Baron M. D., Barrett T. 1997; Rescue of rinderpest virus from cloned cDNA. J Virol 71:1265–1271
    [Google Scholar]
  4. Bellini W. J., Englund G., Rozenblatt S., Arnheiter H., Richardson C. D. 1985; Measles virus P gene codes for two proteins. J Virol 53:908–919
    [Google Scholar]
  5. Calain P., Roux L. 1993; The rule of six, a basic feature for efficient replication of Sendai virus defective interfering RNA. J Virol 67:4822–4830
    [Google Scholar]
  6. Cattaneo R., Rebmann G., Schmid A., Baczko K., ter Meulen V., Billeter M. A. 1987; Altered transcription of a defective measles virus genome derived from a diseased human brain. EMBO J 6:681–688
    [Google Scholar]
  7. Dimock K., Collins P. L. 1993; Rescue of synthetic analogs of genomic RNA and replicative-intermediate RNA of human parainfluenza virus type 3. J Virol 67:2772–2778
    [Google Scholar]
  8. Gassen U., Collins F. M., Duprex W. P., Rima B. K. 2000; Establishment of a rescue system for canine distemper virus. J Virol 74:10737–10744 [CrossRef]
    [Google Scholar]
  9. Karlin D., Ferron F., Canard B., Longhi S. 2003; Structural disorder and modular organization in Paramyxovirinae N and P. J Gen Virol 84:3239–3252 [CrossRef]
    [Google Scholar]
  10. Pelet T., Marq J. B., Sakai Y., Wakao S., Gotoh H., Curran J. 1996; Rescue of Sendai virus cDNA templates with cDNA clones expressing parainfluenza virus type 3 N. P and L proteins. J Gen Virol 77:2465–2469 [CrossRef]
    [Google Scholar]
  11. Radecke F., Spielhofer P., Schneider H., Kaelin K., Huber M., Dotsch C., Christiansen G., Billeter M. A. 1995; Rescue of measles viruses from cloned DNA. EMBO J 14:5773–5784
    [Google Scholar]
  12. Schneider H., Spielhofer P., Kaelin K., Dotsch C., Radecke F., Sutter G., Billeter M. A. 1997; Rescue of measles virus using a replication-deficient vaccinia-T7 vector. J Virol Methods 64:57–64 [CrossRef]
    [Google Scholar]
  13. Taubenberger J. K., Tsai M. M., Atkin T. J., Fanning T. G., Krafft A. E., Moeller R. B., Kodsi S. E., Mense M. G., Lipscomb T. P. 2000; Molecular genetic evidence of a novel morbillivirus in a long-finned pilot whale ( Globicephalus melas ). Emerg Infect Dis 6:42–45 [CrossRef]
    [Google Scholar]
  14. Yunus A. S., Krishnamurthy S., Pastey M. K., Huang Z., Khattar S. K., Collins P. L., Samal S. K. 1999; Rescue of a bovine respiratory syncytial virus genomic RNA analog by bovine, human and ovine respiratory syncytial viruses confirms the “functional integrity” and “cross-recognition” of BRSV cis-acting elements by HRSV and ORSV. Arch Virol 144:1977–1990 [CrossRef]
    [Google Scholar]
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