‘Rescue’ of mini-genomic constructs and viruses by combinations of morbillivirus N, P and L proteins
Brown, D. D. and Collins, F. M. and Duprex, W. P. and Baron, M. D. and Barrett, T. and Rima, B. K.,, 86, 1077-1081 (2005),
doi = https://doi.org/10.1099/vir.0.80804-0,
publicationName = Microbiology Society,
issn = 0022-1317,
abstract= Chloramphenicol acetyltransferase (CAT)-expressing negative-sense mini-genomic constructs of measles virus (MV) and rinderpest virus (RPV) were rescued by standard technology with helper plasmids expressing the nucleocapsid (N), phospho- (P) and large (L) proteins of MV, canine distemper virus (CDV) or RPV in order to determine whether the proteins of different viruses can function together. Homogeneous sets consisting of N, P and L plasmids derived from one virus were able to generate reporter gene expression from either mini-genomic construct. Heterogeneous sets of proteins from different viruses were not functional, with the exception that a low level of activity was obtained when MV N and P protein were combined with RPV L protein in the rescue of the MV mini-genomic construct, or CDV N was combined with RPV P and L in the rescue of the RPV mini-genome. However, only homogeneous sets of plasmids were able to rescue infectious virus from full-length anti-genome-expressing plasmids.,
language=,
type=