1887

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Volume 86, Issue 6

Correlation between positivity for immunoglobulin A antibodies and viraemia of swine hepatitis E virus observed among farm pigs in Japan Free

Abstract

To evaluate the usefulness of detection of antibodies to hepatitis E virus (HEV) to screen for viraemic pigs, serum samples obtained from 1425 1–6-month-old pigs in Japan were tested for swine HEV RNA and IgG, IgM and IgA classes of anti-HEV antibody. Fifty-five (5 %) of the 1071 2–5-month-old pigs were positive for swine HEV RNA, but none of 218 1-month-old pigs or 136 6-month-old pigs had detectable HEV RNA. The prevalence of anti-HEV IgG among the viraemic pigs (67 %, 37/55) was similar to that among the non-viraemic pigs (55 %, 757/1370) and the prevalence of anti-HEV IgM among the viraemic pigs and non-viraemic pigs was 7 and 3 %, respectively. However, anti-HEV IgA was detected significantly more frequently among viraemic pigs than among non-viraemic pigs (55 vs 10 %, <0·0001). These results suggest that anti-HEV IgA is more useful than anti-HEV IgM to screen for viraemic pigs.

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2005-06-01
2024-04-23
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References

  1. Arankalle V. A., Chobe L. P., Joshi M. V., Chadha M. S., Kundu B., Walimbe A. M. 2002; Human and swine hepatitis E viruses from Western India belong to different genotypes. J Hepatol 36:417–425 [CrossRef]
     [Google Scholar]
  2. Chandler J. D., Riddell M. A., Li F., Love R. J., Anderson D. A. 1999; Serological evidence for swine hepatitis E virus infection in Australian pig herds. Vet Microbiol 68:95–105 [CrossRef]
     [Google Scholar]
  3. Chau K. H., Dawson G. J., Bile K. M., Magnius L. O., Sjogren M. H., Mushahwar I. K. 1993; Detection of IgA class antibody to hepatitis E virus in serum samples from patients with hepatitis E virus infection. J Med Virol 40:334–338 [CrossRef]
     [Google Scholar]
  4. Choi I.-S., Kwon H.-J., Shin N.-R., Yoo H. S. 2003; Identification of swine hepatitis E virus (HEV) and prevalence of anti-HEV antibodies in swine and human populations in Korea. J Clin Microbiol 41:3602–3608 [CrossRef]
     [Google Scholar]
  5. Clayson E. T., Innis B. L., Myint K. S. A., Narupiti S., Vaughn D. W., Giri S., Ranabhat P., Shrestha M. P. 1995; Detection of hepatitis E virus infections among domestic swine in the Kathmandu Valley of Nepal. Am J Trop Med Hyg 53:228–232
     [Google Scholar]
  6. Erker J. C., Desai S. M., Schlauder G. G., Dawson G. J., Mushahwar I. K. 1999; A hepatitis E virus variant from the United States: molecular characterization and transmission in cynomolgus macaques. J Gen Virol 80:681–690
     [Google Scholar]
  7. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
     [Google Scholar]
  8. Garkavenko O., Obriadina A., Meng J., Anderson D. A., Benard H. J., Schroeder B. A., Khudyakov Y. E., Fields H. A., Croxson M. C. 2001; Detection and characterization of swine hepatitis E virus in New Zealand. J Med Virol 65:525–529 [CrossRef]
     [Google Scholar]
  9. Halbur P. G., Kasorndorkbua C., Gilbert C., Guenette D., Potters M. B., Purcell R. H., Emerson S. U., Toth T. E., Meng X. J. 2001; Comparative pathogenesis of infection of pigs with hepatitis E viruses recovered from a pig and a human. J Clin Microbiol 39:918–923 [CrossRef]
     [Google Scholar]
  10. Harrison T. J. 1999; Hepatitis E virus – an update. Liver 19:171–176
     [Google Scholar]
  11. Hsieh S.-Y., Meng X.-J., Wu Y.-H., Liu S.-T., Tam A. W., Lin D.-Y., Liaw Y.-F. 1999; Identity of a novel swine hepatitis E virus in Taiwan forming a monophyletic group with Taiwan isolates of human hepatitis E virus. J Clin Microbiol 37:3828–3834
     [Google Scholar]
  12. Huang C.-C., Nguyen D., Fernandez J., Yun K. Y., Fry K. E., Bradley D. W., Tam A. W., Reyes G. R. 1992; Molecular cloning and sequencing of the Mexico isolate of hepatitis E virus (HEV). Virology 191:550–558 [CrossRef]
     [Google Scholar]
  13. Huang F. F., Haqshenas G., Guenette D. K., Halbur P. G., Schommer S. K., Pierson F. W., Toth T. E., Meng X. J. 2002a; Detection by reverse transcription-PCR and genetic characterization of field isolates of swine hepatitis E virus from pigs in different geographic regions of the United States. J Clin Microbiol 40:1326–1332 [CrossRef]
     [Google Scholar]
  14. Huang F. F., Haqshenas G., Shivaprasad H. L. 7 other authors 2002b; Heterogeneity and seroprevalence of a newly identified avian hepatitis E virus from chickens in the United States. J Clin Microbiol 40:4197–4202 [CrossRef]
     [Google Scholar]
  15. Huang F. F., Sun Z. F., Emerson S. U., Purcell R. H., Shivaprasad H. L., Pierson F. W., Toth T. E., Meng X. J. 2004; Determination and analysis of the complete genomic sequence of avian hepatitis E virus (avian HEV) and attempts to infect rhesus monkeys with avian HEV. J Gen Virol 85:1609–1618 [CrossRef]
     [Google Scholar]
  16. Ina Y. 1994; oden: a program package for molecular evolutionary analysis and database search of DNA and amino acid sequences. Comput Appl Biosci 10:11–12
     [Google Scholar]
  17. Meng X.-J. 2000; Novel strains of hepatitis E virus identified from humans and other animal species: is hepatitis E a zoonosis?. J Hepatol 33:842–845 [CrossRef]
     [Google Scholar]
  18. Meng X.-J. 2003; Swine hepatitis E virus: cross-species infection and risk in xenotransplantation. Curr Top Microbiol Immunol 278:185–216
     [Google Scholar]
  19. Meng X.-J., Purcell R. H., Halbur P. G., Lehman J. R., Webb D. M., Tsareva T. S., Haynes J. S., Thacker B. J., Emerson S. U. 1997; A novel virus in swine is closely related to the human hepatitis E virus. Proc Natl Acad Sci U S A 94:9860–9865 [CrossRef]
     [Google Scholar]
  20. Meng X.-J., Halbur P. G., Haynes J. S., Tsareva T. S., Bruna J. D., Royer R. L., Purcell R. H., Emerson S. U. 1998a; Experimental infection of pigs with the newly identified swine hepatitis E virus (swine HEV), but not with human strains of HEV. Arch Virol 143:1405–1415 [CrossRef]
     [Google Scholar]
  21. Meng X.-J., Halbur P. G., Shapiro M. S., Govindarajan S., Bruna J. D., Mushahwar I. K., Purcell R. H., Emerson S. U. 1998b; Genetic and experimental evidence for cross-species infection by swine hepatitis E virus. J Virol 72:9714–9721
     [Google Scholar]
  22. Meng X.-J., Dea S., Engle R. E. 10 other authors 1999; Prevalence of antibodies to the hepatitis E virus in pigs from countries where hepatitis E is common or is rare in the human population. J Med Virol 59:297–302 [CrossRef]
     [Google Scholar]
  23. Mitsui T., Tsukamoto Y., Yamazaki C., Masuko K., Tsuda F., Takahashi M., Nishizawa T., Okamoto H. 2004; Prevalence of hepatitis E virus infection among hemodialysis patients in Japan: evidence for infection with a genotype 3 HEV by blood transfusion. J Med Virol 74:563–572 [CrossRef]
     [Google Scholar]
  24. Mizuo H., Suzuki K., Takikawa Y. 8 other authors 2002; Polyphyletic strains of hepatitis E virus are responsible for sporadic cases of acute hepatitis in Japan. J Clin Microbiol 40:3209–3218 [CrossRef]
     [Google Scholar]
  25. Nishizawa T., Takahashi M., Mizuo H., Miyajima H., Gotanda Y., Okamoto H. 2003; Characterization of Japanese swine and human hepatitis E virus isolates of genotype IV with 99 % identity over the entire genome. J Gen Virol 84:1245–1251 [CrossRef]
     [Google Scholar]
  26. Okamoto H., Takahashi M., Nishizawa T., Fukai K., Muramatsu U., Yoshikawa A. 2001; Analysis of the complete genome of indigenous swine hepatitis E virus isolated in Japan. Biochem Biophys Res Commun 289:929–936 [CrossRef]
     [Google Scholar]
  27. Okamoto H., Takahashi M., Nishizawa T. 2003; Features of hepatitis E virus infection in Japan. Intern Med 42:1065–1071 [CrossRef]
     [Google Scholar]
  28. Pei Y., Yoo D. 2002; Genetic characterization and sequence heterogeneity of a Canadian isolate of swine hepatitis E virus. J Clin Microbiol 40:4021–4029 [CrossRef]
     [Google Scholar]
  29. Pina S., Buti M., Cotrina M., Piella J., Girones R. 2000; HEV identified in serum from humans with acute hepatitis and in sewage of animal origin in Spain. J Hepatol 33:826–833 [CrossRef]
     [Google Scholar]
  30. Purcell R. H., Emerson S. U. 2001; Hepatitis E virus. In Fields Virology . , 4th edn. pp  3051–3061 Edited by Knipe D. M., Howley P. M., Griffin D. E., Lamb R. A., Martin M. A., Roizman B., Straus S. E. Philadelphia, PA: Lippincott Williams & Wilkins;
  31. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
     [Google Scholar]
  32. Schlauder G. G., Mushahwar I. K. 2001; Genetic heterogeneity of hepatitis E virus. J Med Virol 65:282–292 [CrossRef]
     [Google Scholar]
  33. Smith J. L. 2001; A review of hepatitis E virus. J Food Prot 64:572–586
     [Google Scholar]
  34. Takahashi M., Nishizawa T., Miyajima H., Gotanda Y., Iita T., Tsuda F., Okamoto H. 2003a; Swine hepatitis E virus strains in Japan form four phylogenetic clusters comparable with those of Japanese isolates of human hepatitis E virus. J Gen Virol 84:851–862 [CrossRef]
     [Google Scholar]
  35. Takahashi M., Nishizawa T., Okamoto H. 2003b; Identification of a genotype III swine hepatitis E virus that was isolated from a Japanese pig born in 1990 and that is most closely related to Japanese isolates of human hepatitis E virus. J Clin Microbiol 41:1342–1343 [CrossRef]
     [Google Scholar]
  36. Takahashi M., Kusakai S., Mizuo H. 7 other authors 2005; Simultaneous detection of immunoglobulin A (IgA) and IgM antibodies against hepatitis E virus (HEV) is highly specific for diagnosis of acute HEV infection. J Clin Microbiol 43:49–56 [CrossRef]
     [Google Scholar]
  37. Tam A. W., Smith M. M., Guerra M. E., Huang C.-C., Bradley D. W., Fry K. E., Reyes G. R. 1991; Hepatitis E virus (HEV): molecular cloning and sequencing of the full-length viral genome. Virology 185:120–131 [CrossRef]
     [Google Scholar]
  38. Tanaka H., Yoshino H., Kobayashi E., Takahashi M., Okamoto H. 2004; Molecular investigation of hepatitis E virus infection in domestic and miniature pigs used for medical experiments. Xenotransplantation 11:503–510 [CrossRef]
     [Google Scholar]
  39. Tei S., Kitajima N., Takahashi K., Mishiro S. 2003; Zoonotic transmission of hepatitis E virus from deer to human beings. Lancet 362:371–373 [CrossRef]
     [Google Scholar]
  40. Tokita H., Harada H., Gotanda Y., Takahashi M., Nishizawa T., Okamoto H. 2003; Molecular and serological characterization of sporadic acute hepatitis E in a Japanese patient infected with a genotype III hepatitis E virus in 1993. J Gen Virol 84:421–427 [CrossRef]
     [Google Scholar]
  41. Usui R., Kobayashi E., Takahashi M., Nishizawa T., Okamoto H. 2004; Presence of antibodies to hepatitis E virus in Japanese pet cats. Infection 32:57–58 [CrossRef]
     [Google Scholar]
  42. van der Poel W. H., Verschoor F., van der Heide R., Herrera M.-I., Vivo A., Kooreman M., de Roda Husman A. M. 2001; Hepatitis E virus sequences in swine related to sequences in humans, the Netherlands. Emerg Infect Dis 7:970–976 [CrossRef]
     [Google Scholar]
  43. Wang Y., Zhang H., Ling R., Li H., Harrison T. J. 2000; The complete sequence of hepatitis E virus genotype 4 reveals an alternative strategy for translation of open reading frames 2 and 3. J Gen Virol 81:1675–1686
     [Google Scholar]
  44. Wang Y.-C., Zhang H., Xia N. 11 other authors 2002; Prevalence, isolation, and partial sequence analysis of hepatitis E virus from domestic animals in China. J Med Virol 67:516–521 [CrossRef]
     [Google Scholar]
  45. Wibawa I. D. N., Muljono D. H., Mulyanto Suryadarma., A I. G, Tsuda F., Takahashi M., Nishizawa T., Okamoto H. 2004; Prevalence of antibodies to hepatitis E virus among apparently healthy humans and pigs in Bali, Indonesia: identification of a pig infected with a genotype 4 hepatitis E virus. J Med Virol 73:38–44 [CrossRef]
     [Google Scholar]
  46. Wu J.-C., Chen C.-M., Chiang T.-Y., Tsai W.-H., Jeng W.-J., Sheen I.-J., Kin C.-C., Meng X.-J. 2002; Spread of hepatitis E virus among different-aged pigs: two-year survey in Taiwan. J Med Virol 66:488–492 [CrossRef]
     [Google Scholar]
  47. Yazaki Y., Mizuo H., Takahashi M., Nishizawa T., Sasaki N., Gotanda Y., Okamoto H. 2003; Sporadic acute or fulminant hepatitis E in Hokkaido, Japan, may be food-borne, as suggested by the presence of hepatitis E virus in pig liver as food. J Gen Virol 84:2351–2357 [CrossRef]
     [Google Scholar]
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Correlation between positivity for immunoglobulin A antibodies and viraemia of swine hepatitis E virus observed among farm pigs in Japan
J. Gen. Virol. 86, 1807 (2005); https://doi.org/10.1099/vir.0.80909-0
/content/journal/jgv/10.1099/vir.0.80909-0
/content/journal/jgv/10.1099/vir.0.80909-0
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