@article{mbs:/content/journal/jgv/10.1099/vir.0.80955-0, author = "Fukushi, Shuetsu and Mizutani, Tetsuya and Saijo, Masayuki and Matsuyama, Shutoku and Miyajima, Naoko and Taguchi, Fumihiro and Itamura, Shigeyuki and Kurane, Ichiro and Morikawa, Shigeru", title = "Vesicular stomatitis virus pseudotyped with severe acute respiratory syndrome coronavirus spike protein", journal= "Journal of General Virology", year = "2005", volume = "86", number = "8", pages = "2269-2274", doi = "https://doi.org/10.1099/vir.0.80955-0", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.80955-0", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "Severe acute respiratory syndrome coronavirus (SARS-CoV) contains a single spike (S) protein, which binds to its receptor, angiotensin-converting enzyme 2 (ACE2), induces membrane fusion and serves as a neutralizing antigen. A SARS-CoV-S protein-bearing vesicular stomatitis virus (VSV) pseudotype using the VSVΔG* system was generated. Partial deletion of the SARS-CoV-S protein cytoplasmic domain allowed efficient incorporation into VSV particles and led to the generation of a pseudotype (VSV-SARS-St19) at high titre. Green fluorescent protein expression was demonstrated as early as 7 h after infection of Vero E6 cells with VSV-SARS-St19. VSV-SARS-St19 was neutralized by anti-SARS-CoV antibody and soluble ACE2, and its infection was blocked by treatment of Vero E6 cells with anti-ACE2 antibody. These results indicated that VSV-SARS-St19 infection is mediated by SARS-CoV-S protein in an ACE2-dependent manner. VSV-SARS-St19 will be useful for analysing the function of SARS-CoV-S protein and for developing rapid methods of detecting neutralizing antibodies specific for SARS-CoV infection.", }