1887

Abstract

The purpose of this experiment was to study the immune response of pigs during an experimental infection with a European strain of (PRRSV). Five pigs were challenged intranasally with PRRSV strain VP21 and another five were kept as controls. Clinical course and humoral and cell-mediated responses were monitored for 70 days post-infection (p.i.). Infected pigs developed mild signs at 24 h p.i. Viraemia was detectable by nested RT-PCR until day 14 p.i. Earliest seroconversions (ELISA) were seen by day 7 p.i. (three of five animals) and, by day 14, all inoculated pigs had seroconverted (ELISA and immunoperoxidase monolayer assay). Virus-neutralizing antibodies were undetectable until day 56 p.i. and, by day 70 p.i., two inoculated pigs still were negative. Flow-cytometry assays using peripheral blood mononuclear cells (PBMC) showed an upshift in CD8 cells (day 7 p.i.) and a downshift of CD21 cells (days 7 and 28 p.i.). Regarding cell-mediated responses, development of PRRSV-specific gamma interferon-secreting cells (IFN--SC) and interleukin 4-secreting cells (IL4-SC) in PBMC was examined by ELISPOT assay. IFN--SC were not detected significantly until day 14 p.i., whereas, for IL4-SC, no differences between groups were seen. Concurrently with the onset of viraemia and the development of clinical signs, serum haptoglobin levels and interleukin 10 (IL10) in PRRSV-stimulated PBMC-culture supernatants increased significantly. These differences disappeared later on. For IL2, IL4, IL8 or transforming growth factor beta, no differences were seen among groups. These results are compatible with a model in which the immune response does not fully control the outcome of the infection.

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2005-07-01
2024-03-29
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References

  1. Aasted B., Bach P., Nielsen J., Lind P. 2002; Cytokine profiles in peripheral blood mononuclear cells and lymph node cells from piglets infected in utero with porcine reproductive and respiratory syndrome virus. Clin Diagn Lab Immunol 9:1229–1234
    [Google Scholar]
  2. Albina E., Piriou L., Hutet E., Cariolet R., L'Hospitalier R. 1998; Immune responses in pigs infected with porcine reproductive and respiratory syndrome virus (PRRSV). Vet Immunol Immunopathol 61:49–66 [CrossRef]
    [Google Scholar]
  3. Asai T., Mori M., Okada M., Uruno K., Yazawa S., Shibata I. 1999; Elevated serum haptoglobin in pigs infected with porcine reproductive and respiratory syndrome virus. Vet Immunol Immunopathol 70:143–148 [CrossRef]
    [Google Scholar]
  4. Batista L., Pijoan C., Dee S., Olin M., Molitor T., Joo H. S., Xiao Z., Murtaugh M. 2004; Virological and immunological responses to porcine reproductive and respiratory syndrome virus in a large population of gilts. Can J Vet Res 68:267–273
    [Google Scholar]
  5. Chung H.-K., Chae C. 2003; Expression of interleukin-10 and interleukin-12 in piglets experimentally infected with porcine reproductive and respiratory syndrome virus (PRRSV). J Comp Pathol 129:205–212 [CrossRef]
    [Google Scholar]
  6. Darwich L., Balasch M., Plana-Durán J., Segalés J., Domingo M., Mateu E. 2003; Cytokine profiles of peripheral blood mononuclear cells from pigs with postweaning multisystemic wasting syndrome in response to mitogen, superantigen or recall viral antigens. J Gen Virol 84:3453–3457 [CrossRef]
    [Google Scholar]
  7. Dea S., Gagnon C. A., Mardassi H., Pirzadeh B., Rogan D. 2000; Current knowledge on the structural proteins of porcine reproductive and respiratory syndrome (PRRS) virus: comparison of the North American and European isolates. Arch Virol 145:659–688 [CrossRef]
    [Google Scholar]
  8. Díaz I., Mateu E. 2005; Use of ELISPOT and ELISA to evaluate IFN- γ , IL-10 and IL-4 responses in conventional pigs. Vet Immunol Immunopathol (in press
    [Google Scholar]
  9. Drew T. W., Lowings J. P., Yapp F. 1997; Variation in open reading frames 3, 4 and 7 among porcine reproductive and respiratory syndrome virus isolates in the UK. Vet Microbiol 55:209–221 [CrossRef]
    [Google Scholar]
  10. Duan X., Nauwynck H. J., Pensaert M. B. 1997; Virus quantification and identification of cellular targets in the lungs and lymphoid tissues of pigs at different time intervals after inoculation with porcine reproductive and respiratory syndrome virus (PRRSV). Vet Microbiol 56:9–19 [CrossRef]
    [Google Scholar]
  11. Forsberg R., Storgaard T., Nielsen H. S., Oleksiewicz M. B., Cordioli P., Sala G., Hein J., Bøtner A. 2002; The genetic diversity of European type PRRSV is similar to that of the North American type but is geographically skewed within Europe. Virology 299:38–47 [CrossRef]
    [Google Scholar]
  12. Gagnon C. A., Dea S. 1998; Differentiation between porcine reproductive and respiratory syndrome virus isolates by restriction fragment length polymorphism of their ORFs 6 and 7 genes. Can J Vet Res 62:110–116
    [Google Scholar]
  13. Goldberg T. L., Lowe J. F., Milburn S. M., Firkins L. D. 2003; Quasispecies variation of porcine reproductive and respiratory syndrome virus during natural infection. Virology 317:197–207 [CrossRef]
    [Google Scholar]
  14. Johnson W., Roof M., Vaughn E., Christopher-Hennings J., Johnson C. R., Murtaugh M. P. 2004; Pathogenic and humoral immune responses to porcine reproductive and respiratory syndrome virus (PRRSV) are related to viral load in acute infection. Vet Immunol Immunopathol 102:233–247 [CrossRef]
    [Google Scholar]
  15. Jusa E. R., Inaba Y., Kouno M., Hirose O., Shibata I., Kubota M., Yasuhara H. 1996; Slow-reacting and complement-requiring neutralizing antibody in swine infected with porcine reproductive and respiratory syndrome (PRRS) virus. J Vet Med Sci 58:749–753 [CrossRef]
    [Google Scholar]
  16. Kawashima K., Narita M., Yamada S. 1999; Changes in macrophage and lymphocyte subpopulations of lymphoid tissues from pigs infected with the porcine reproductive and respiratory syndrome virus (PRRSV). Vet Immunol Immunopathol 71:257–262 [CrossRef]
    [Google Scholar]
  17. Labarque G., Van Gucht S., Van Reeth K., Nauwynck H., Pensaert M. 2003; Respiratory tract protection upon challenge of pigs vaccinated with attenuated porcine reproductive and respiratory syndrome virus vaccines. Vet Microbiol 95:187–197 [CrossRef]
    [Google Scholar]
  18. Lager K. M., Mengeling W. L. 2000; PRRS: nature of the RNA virus and how it causes disease (a keynote paper). In Proceedings of the 16th International Pig Veterinary Society Congress pp  538–543 17–20 September 2000 Melbourne, Australia:
    [Google Scholar]
  19. Lager K. M., Mengeling W. L., Brockmeier S. L. 1999; Evaluation of protective immunity in gilts inoculated with the NADC-8 isolate of porcine reproductive and respiratory syndrome virus (PRRSV) and challenge-exposed with an antigenically distinct PRRSV isolate. Am J Vet Res 60:1022–1027
    [Google Scholar]
  20. Lamontagne L., Page C., Larochelle R., Longtin D., Magar R. 2001; Polyclonal activation of B cells occurs in lymphoid organs from porcine reproductive and respiratory syndrome virus (PRRSV)-infected pigs. Vet Immunol Immunopathol 82:165–182 [CrossRef]
    [Google Scholar]
  21. Loemba H. D., Mounir S., Mardassi H., Archambault D., Dea S. 1996; Kinetics of humoral immune response to the major structural proteins of the porcine reproductive and respiratory syndrome virus. Arch Virol 141:751–761 [CrossRef]
    [Google Scholar]
  22. Lopez O. J., Osorio F. A. 2004; Role of neutralizing antibodies in PRRSV protective immunity. Vet Immunol Immunopathol 102:155–163 [CrossRef]
    [Google Scholar]
  23. López Fuertes L., Doménech N., Alvarez B., Ezquerra A., Domínguez J., Castro J. M., Alonso F. 1999; Analysis of cellular immune response in pigs recovered from porcine respiratory and reproductive syndrome infection. Virus Res 64:33–42 [CrossRef]
    [Google Scholar]
  24. Mateu E., Martín M., Vidal D. 2003; Genetic diversity and phylogenetic analysis of glycoprotein 5 of European-type porcine reproductive and respiratory virus strains in Spain. J Gen Virol 84:529–534 [CrossRef]
    [Google Scholar]
  25. McGuirk P., Mills K. H. G. 2002; Pathogen-specific regulatory T cells provoke a shift in the Th1/Th2 paradigm in immunity to infectious diseases. Trends Immunol 23:450–455 [CrossRef]
    [Google Scholar]
  26. Meier W. A., Galeota J., Osorio F. A., Husmann R. J., Schnitzlein W. M., Zuckermann F. A. 2003; Gradual development of the interferon- γ response of swine to porcine reproductive and respiratory syndrome virus infection or vaccination. Virology 309:18–31 [CrossRef]
    [Google Scholar]
  27. Meng X. J. 2000; Heterogeneity of porcine reproductive and respiratory syndrome virus: implications for current vaccine efficacy and future vaccine development. Vet Microbiol 74:309–329 [CrossRef]
    [Google Scholar]
  28. Meng X. J., Paul P. S., Halbur P. G., Lum M. A. 1995; Phylogenetic analyses of the putative M (ORF 6) and N (ORF 7) genes of porcine reproductive and respiratory syndrome virus (PRRSV): implication for the existence of two genotypes of PRRSV in the U.S.A. and Europe. Arch Virol 140:745–755 [CrossRef]
    [Google Scholar]
  29. Mengeling W. L., Lager K. M., Vorwald A. C., Koehler K. J. 2003; Strain specificity of the immune response of pigs following vaccination with various strains of porcine reproductive and respiratory syndrome virus. Vet Microbiol 93:13–24 [CrossRef]
    [Google Scholar]
  30. Meulenberg J. J., Hulst M. M., de Meijer E. J., Moonen P. L., den Besten A., de Kluyver E. P., Wensvoort G., Moormann R. J. 1994; Lelystad virus belongs to a new virus family, comprising lactate dehydrogenase-elevating virus, equine arteritis virus, and simian hemorrhagic fever virus. Arch Virol Suppl 9:441–448
    [Google Scholar]
  31. Moestrup S. K., Moller H. J. 2004; CD163: a regulated hemoglobin scavenger receptor with a role in the anti-inflammatory response. Ann Med 36:347–354 [CrossRef]
    [Google Scholar]
  32. Molitor T. W., Bautista E. M., Choi C. S. 1997; Immunity to PRRSV: double-edged sword. Vet Microbiol 55:265–276 [CrossRef]
    [Google Scholar]
  33. Moore K. W., de Waal Malefyt R., Coffman R. L., O'Garra A. 2001; Interleukin-10 and the interleukin-10 receptor. Annu Rev Immunol 19:683–765 [CrossRef]
    [Google Scholar]
  34. Murtaugh M. P., Elam M. R., Kakach L. T. 1995; Comparison of the structural protein coding sequences of the VR-2332 and Lelystad virus strains of the PRRS virus. Arch Virol 140:1451–1460 [CrossRef]
    [Google Scholar]
  35. Murtaugh M. P., Xiao Z., Zuckermann F. 2002; Immunological responses of swine to porcine reproductive and respiratory syndrome virus infection. Viral Immunol 15:533–547 [CrossRef]
    [Google Scholar]
  36. Nielsen J., Bøtner A. 1997; Hematological and immunological parameters of 4½-month old pigs infected with PRRS virus. Vet Microbiol 55:289–294 [CrossRef]
    [Google Scholar]
  37. Onyekaba C. O., Harty J. T., Even C., Hu B.-G., Plagemann P. G. W. 1989; Persistent infection of mice by lactate dehydrogenase-elevating virus: effects of immunosuppression on virus replication and antiviral immune responses. Virus Res 14:297–315 [CrossRef]
    [Google Scholar]
  38. Osorio F. A., Galeota J. A., Nelson E., Brodersen B., Doster A., Wills R., Zuckermann F., Laegreid W. W. 2002; Passive transfer of virus-specific antibodies confers protection against reproductive failure induced by a virulent strain of porcine reproductive and respiratory syndrome virus and establishes sterilizing immunity. Virology 302:9–20 [CrossRef]
    [Google Scholar]
  39. Ostrowski M., Galeota J. A., Jar A. M., Platt K. B., Osorio F. A., Lopez O. J. 2002; Identification of neutralizing and nonneutralizing epitopes in the porcine reproductive and respiratory syndrome virus GP5 ectodomain. J Virol 76:4241–4250 [CrossRef]
    [Google Scholar]
  40. Philippidis P., Mason J. C., Evans B. J., Nadra I., Taylor K. M., Haskard D. O., Landis R. C. 2004; Hemoglobin scavenger receptor CD163 mediates interleukin-10 release and heme oxygenase-1 synthesis: antiinflammatory monocyte-macrophage responses in vitro, in resolving skin blisters in vivo, and after cardiopulmonary bypass surgery. Circ Res 94:119–126 [CrossRef]
    [Google Scholar]
  41. Royaee A. R., Husmann R. J., Dawson H. D., Calzada-Nova G., Schnitzlein W. M., Zuckermann F. A., Lunney J. K. 2004; Deciphering the involvement of innate immune factors in the development of the host response to PRRSV vaccination. Vet Immunol Immunopathol 102:199–216 [CrossRef]
    [Google Scholar]
  42. Samsom J. N., de Bruin T. G. M., Voermans J. J. M., Meulenberg J. J. M., Pol J. M. A., Bianchi A. T. J. 2000; Changes of leukocyte phenotype and function in the broncho-alveolar lavage fluid of pigs infected with porcine reproductive and respiratory syndrome virus: a role for CD8+ cells. J Gen Virol 81:497–505
    [Google Scholar]
  43. Sánchez-Torres C., Gómez-Puertas P., Gómez-del-Moral M., Alonso F., Escribano J. M., Ezquerra A., Domínguez J. 2003; Expression of porcine CD163 on monocytes/macrophages correlates with permissiveness to African swine fever infection. Arch Virol 148:2307–2323 [CrossRef]
    [Google Scholar]
  44. Shimizu M., Yamada S., Kawashima K., Ohashi S., Shimizu S., Ogawa T. 1996; Changes of lymphocyte subpopulations in pigs infected with porcine reproductive and respiratory syndrome (PRRS) virus. Vet Immunol Immunopathol 50:19–27 [CrossRef]
    [Google Scholar]
  45. Sipos W., Duvigneau C., Pietschmann P. 7 other authors 2003; Parameters of humoral and cellular immunity following vaccination of pigs with a European modified-live strain of porcine reproductive and respiratory syndrome virus (PRRSV). Viral Immunol 16:335–346 [CrossRef]
    [Google Scholar]
  46. Suradhat S., Thanawongnuwech R. 2003; Upregulation of interleukin-10 gene expression in the leukocytes of pigs infected with porcine reproductive and respiratory syndrome virus. J Gen Virol 84:2755–2760 [CrossRef]
    [Google Scholar]
  47. Suradhat S., Thanawongnuwech R., Poovorawan Y. 2003; Upregulation of IL-10 gene expression in porcine peripheral blood mononuclear cells by porcine reproductive and respiratory syndrome virus. J Gen Virol 84:453–459 [CrossRef]
    [Google Scholar]
  48. van der Linden I. F. A., Voermans J. J. M., van der Linde-Bril E. M., Bianchi A. T. J., Steverink P. J. G. M. 2003; Virological kinetics and immunological responses to a porcine reproductive and respiratory syndrome virus infection of pigs at different ages. Vaccine 21:1952–1957 [CrossRef]
    [Google Scholar]
  49. van Woensel P. A., Liefkens K., Demaret S. 1998; European serotype PRRSV vaccine protects against European serotype challenge whereas an American serotype vaccine does not. Adv Exp Med Biol 440:713–718
    [Google Scholar]
  50. Vezina S. A., Loemba H., Fournier M., Dea S., Archambault D. 1996; Antibody production and blastogenic response in pigs experimentally infected with porcine reproductive and respiratory syndrome virus. Can J Vet Res 60:94–99
    [Google Scholar]
  51. Wensvoort G., Terpstra C., Pol J. M. 19 other authors 1991; Mystery swine disease in the Netherlands: the isolation of Lelystad virus. Vet Q 13:121–130 [CrossRef]
    [Google Scholar]
  52. Xiao Z., Batista L., Dee S., Halbur P., Murtaugh M. P. 2004; The level of virus-specific T-cell and macrophage recruitment in porcine reproductive and respiratory syndrome virus infection in pigs is independent of virus load. J Virol 78:5923–5933 [CrossRef]
    [Google Scholar]
  53. Yoon I. J., Joo H. S., Goyal S. M., Molitor T. W. 1994; A modified serum neutralization test for the detection of antibody to porcine reproductive and respiratory syndrome virus in swine sera. J Vet Diagn Invest 6:289–292
    [Google Scholar]
  54. Yoon K. J., Zimmerman J. J., Swenson S. L. 7 other authors 1995; Characterization of the humoral immune response to porcine reproductive and respiratory syndrome (PRRS) virus infection. J Vet Diagn Invest 7:305–312 [CrossRef]
    [Google Scholar]
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