1887

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Volume 86, Issue 11

An model for the regulation of human cytomegalovirus latency and reactivation in dendritic cells by chromatin remodelling Free

Abstract

Human cytomegalovirus (HCMV) is a frequent cause of major disease following primary infection or reactivation from latency in immunocompromised patients. Infection of non-permissive mononuclear cells is used for analyses of HCMV latency . Using this approach, it is shown here that repression of lytic gene expression following experimental infection of CD34 cells, a site of HCMV latency , correlates with recruitment of repressive chromatin around the major immediate-early promoter (MIEP). Furthermore, long-term culture of CD34 cells results in carriage of viral genomes in which the MIEP remains associated with transcriptionally repressive chromatin. Finally, specific differentiation of long-term cultures of infected CD34 cells to mature dendritic cells results in acetylation of histones bound to the MIEP, concomitant loss of heterochromatin protein 1 and the reactivation of HCMV. These data are consistent with analyses of latency and may provide a model for further analyses of the mechanisms involved during latency and reactivation.

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2005-11-01
2024-04-20
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An in vitro model for the regulation of human cytomegalovirus latency and reactivation in dendritic cells by chromatin remodelling
J. Gen. Virol. 86, 2949 (2005); https://doi.org/10.1099/vir.0.81161-0
/content/journal/jgv/10.1099/vir.0.81161-0
/content/journal/jgv/10.1099/vir.0.81161-0
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