@article{mbs:/content/journal/jgv/10.1099/vir.0.81312-0, author = "Kelly, Barbara J. and King, Linda A. and Possee, Robert D. and Chapple, Susan D. J.", title = "Dual mutations in the Autographa californica nucleopolyhedrovirus FP-25 and p35 genes result in plasma-membrane blebbing in Trichoplusia ni cells", journal= "Journal of General Virology", year = "2006", volume = "87", number = "3", pages = "531-536", doi = "https://doi.org/10.1099/vir.0.81312-0", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.81312-0", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = " Spodoptera frugiperda cells infected with Autographa californica nucleopolyhedrovirus (AcMNPV) lacking a functional anti-apoptotic p35 protein undergo apoptosis. However, such mutants replicate normally in Trichoplusia ni (TN-368) cells. An AcMNPV plaque isolate (AcdefrT) was identified during propagation of a virus deficient in p35 in TN-368 cells. This virus exhibited enhanced budded-particle formation in TN-368 cells, but was partially defective for polyhedra production in the same cells. Virus replication in AcdefrT-infected TN-368 cells was accompanied by extensive plasma-membrane blebbing and caspase activation late in infection, both features of apoptosis. Rescue of the p35 locus of AcdefrT continued to result in a reduction in polyhedra and increase in budded virus production in TN-368 cells, but no plasma-membrane blebbing was observed. The mutation was mapped to the FP-25 gene locus. This gene mutation combined with the non-functional p35 was found to be responsible for the cell-blebbing effect observed in AcdefrT-infected TN-368 cells.", }