1887

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Volume 87, Issue 3

Human herpesvirus 7 U47 gene products are glycoproteins expressed in virions and associate with glycoprotein H Free

Abstract

The function of the human herpesvirus 7 (HHV-7) U47 gene, which is a positional homologue of the genes encoding glycoprotein O (gO) in human cytomegalovirus (HCMV) and human herpesvirus 6 (HHV-6), was analysed. A monoclonal antibody (mAb) against the U47 gene product reacted in immunoblots with proteins migrating at 49 and 51 kDa in lysates of HHV-7-infected cells and with 49 and 51 kDa proteins in partially purified virions. Digestion of the 49 and 51 kDa proteins with endoglycosidase H and peptide -glycosidase F indicated that the U47-encoded proteins were modified with -linked oligosaccharides. Therefore, the U47 gene and its product were named gO, as in HCMV and HHV-6. In addition, the anti-gO mAb co-immunoprecipitated glycoprotein H (gH) in HHV-7-infected cells, indicating an association between HHV-7 gO and gH. The results suggest that the HHV-7 gO–gH complex might have a similar function to that in HCMV or HHV-6, such as cell–cell fusion in virus infection.

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2006-03-01
2024-04-19
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Human herpesvirus 7 U47 gene products are glycoproteins expressed in virions and associate with glycoprotein H
J. Gen. Virol. 87, 501 (2006); https://doi.org/10.1099/vir.0.81374-0
/content/journal/jgv/10.1099/vir.0.81374-0
/content/journal/jgv/10.1099/vir.0.81374-0
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