1887

Abstract

The potyvirus coat protein (CP) is involved in aphid transmission, cell-to-cell movement and virus assembly, not only by binding to viral RNA, but also by self-interaction or interactions with other factors. In this study, a number of CP mutants of (SMV) containing deletions and site-directed mutations were generated and cloned into yeast two-hybrid vectors. Interaction was confirmed by the expression of reporter genes, including , and , in yeast strain AH109. Deletion of the C-terminal region of the CP caused loss of the CP–CP self-interaction ability detected in CP mutants with the C-terminal region. Alanine substitution at the amino acid positions R190, E191, E212, R245, H246 and R249 disrupted CP–CP interaction, whereas substitutions at the amino acid positions R188, D189, D198, K205, K218 and D250 did not. These results indicate that the C-terminal region of SMV CP may contain a domain(s) or amino acids required for CP–CP interaction and virus assembly.

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2006-01-01
2024-03-28
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References

  1. Blanc S., López-Moya J.-J., Wang R., García-Lampasona S., Thornbury D. W., Pirone T. P. 1997; A specific interaction between coat protein and helper component correlates with aphid transmission of a potyvirus. Virology 231:141–147 [CrossRef]
    [Google Scholar]
  2. Carrington J. C., Jensen P. E., Schaad M. C. 1998; Genetic evidence for an essential role for potyvirus CI protein in cell-to-cell movement. Plant J 14:393–400 [CrossRef]
    [Google Scholar]
  3. Flasinski S., Cassidy B. G. 1998; Potyvirus aphid transmission requires helper component and homologous coat protein for maximal efficiency. Arch Virol 143:2159–2172 [CrossRef]
    [Google Scholar]
  4. Guo D., Merits A., Saarma M. 1999; Self-association and mapping of interaction domains of helper component-proteinase of potato A potyvirus. J Gen Virol 80:1127–1131
    [Google Scholar]
  5. Guo D., Rajamäki M.-L., Saarma M., Valkonen J. P. T. 2001; Towards a protein interaction map of potyviruses: protein interaction matrixes of two potyviruses based on the yeast two-hybrid system. J Gen Virol 82:935–939
    [Google Scholar]
  6. Hill J. H. 1999; Soybean mosaic virus. In Compendium of Soybean Diseases , 4th edn. pp  70–71 Edited by Hartman G. L., Sinclair J. B., Rupe J. C. St Paul, MN: American Phytopathological Society;
    [Google Scholar]
  7. Hong Y., Levay K., Murphy J. F., Klein P. G., Shaw J. G., Hunt A. G. 1995; A potyvirus polymerase interacts with the viral coat protein and VPg in yeast cells. Virology 214:159–166 [CrossRef]
    [Google Scholar]
  8. Jayaram Ch., Hill J. H., Miller W. A. 1992; Complete nucleotide sequences of two Soybean mosaic virus strains differentiated by response of soybean containing the Rsv resistance gene. J Gen Virol 73:2067–2077 [CrossRef]
    [Google Scholar]
  9. Kang S.-H., Lim W.-S., Kim K.-H. 2004; A protein interaction map of soybean mosaic virus strain G7H based on the yeast two-hybrid system. Mol Cells 18:122–126
    [Google Scholar]
  10. Kwon S.-J., Park M.-R., Kim K.-W., Plante C. A., Hemenway C. L., Kim K.-H. 2005; cis -Acting sequences required for coat protein binding and in vitro assembly of Potato virus X . Virology 334:83–97 [CrossRef]
    [Google Scholar]
  11. Li X. H., Valdez P., Olvera R. E., Carrington J. C. 1997; Functions of the tobacco etch virus RNA polymerase (NIb): subcellular transport and protein-protein interaction with VPg/proteinase (NIa). J Virol 71:1598–1607
    [Google Scholar]
  12. López-Moya J. J., Wang R. Y., Pirone T. P. 1999; Context of the coat protein DAG motif affects potyvirus transmissibility by aphids. J Gen Virol 80:3281–3288
    [Google Scholar]
  13. Peng Y., Kadoury D., Gal-On A., Huet H., Wang Y., Raccah B. 1998; Mutations in the HC-Pro gene of zucchini yellow mosaic potyvirus: effects on aphid transmission and binding to purified virions. J Gen Virol 79:897–904
    [Google Scholar]
  14. Picard V., Ersdal-Badju E., Lu A., Bock S. C. 1994; A rapid and efficient one-tube PCR-based mutagenesis technique using Pfu DNA polymerase. Nucleic Acids Res 22:2587–2591 [CrossRef]
    [Google Scholar]
  15. Riechmann J. L., Laín S., García J. A. 1992; Highlights and prospects of potyvirus molecular biology. J Gen Virol 73:1–16 [CrossRef]
    [Google Scholar]
  16. Rojas M. R., Zerbini F. M., Allison R. F., Gilbertson R. L., Lucas W. J. 1997; Capsid protein and helper component-proteinase function as potyvirus cell-to-cell movement proteins. Virology 237:283–295 [CrossRef]
    [Google Scholar]
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