1887

Abstract

Human adenovirus type 3 (HAdV-3) is a causative agent of acute respiratory disease, which is prevalent throughout the world, especially in Asia. Here, the complete genome sequences of two field strains of HAdV-3 (strains GZ1 and GZ2) isolated from children with acute respiratory infection in southern China are reported (GenBank accession nos DQ099432 and DQ105654, respectively). The genomes were 35 273 bp (GZ1) and 35 269 bp (GZ2) and both had a G+C content of 51 mol%. They shared 99 % nucleotide identity and the four early and five late regions that are characteristic of human adenoviruses. Thirty-nine protein- and two RNA-coding sequences were identified in the genome sequences of both strains. Protein pX had a predicted molecular mass of 8.3 kDa in strain GZ1; this was lower (7.6 kDa) in strain GZ2. Both strains contained 10 short inverted repeats, in addition to their inverted terminal repeats (111 bp). Comparative whole-genome analysis revealed 93 mismatches and four insertions/deletions between the two strains. Strain GZ1 infection produced a typical cytopathic effect, whereas strain GZ2 did not; non-synonymous substitutions in proteins of GZ2 may be responsible for this difference.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.81515-0
2006-06-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/87/6/1531.html?itemId=/content/journal/jgv/10.1099/vir.0.81515-0&mimeType=html&fmt=ahah

References

  1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. 1990; Basic local alignment search tool. J Mol Biol 215:403–410 [CrossRef]
    [Google Scholar]
  2. Andrade F., Bull H. G., Thornberry N. A., Ketner G. W., Casciola-Rosen L. A., Rosen A. 2001; Adenovirus L4-100K assembly protein is a granzyme B substrate that potently inhibits granzyme B-mediated cell death. Immunity 14:751–761 [CrossRef]
    [Google Scholar]
  3. Benkő M., Harrach B., Russell W. C. 2000; Family Adenoviridae . In Virus Taxonomy: Seventh Report of the International Committee on Taxonomy of Viruses pp  227–238 Edited by van Regenmortel M. H. V., Fauquet C. M., Bishop D. H. L., Carstens E. B., Estes M. K., Lemon S. M., Maniloff J., Mayo M. A., McGeoch D. J., Pringle C. R., Wickner R. B. San Diego: Academic Press;
    [Google Scholar]
  4. Benkő M., Elő P., Ursu K., Ahne W., LaPatra S. E., Thomson D., Harrach B. 2002; First molecular evidence for the existence of distinct fish and snake adenoviruses. J Virol 76:10056–10059 [CrossRef]
    [Google Scholar]
  5. Berriman M., Rutherford K. 2003; Viewing and annotating sequence data with Artemis. Brief Bioinform 4:124–132 [CrossRef]
    [Google Scholar]
  6. Besemer J., Borodovsky M. 1999; Heuristic approach to deriving models for gene finding. Nucleic Acids Res 27:3911–3920 [CrossRef]
    [Google Scholar]
  7. Brendel V., Kleffe J. 1998; Prediction of locally optimal splice sites in plant pre-mRNA with applications to gene identification in Arabidopsis thaliana genomic DNA. Nucleic Acids Res 26:4748–4757 [CrossRef]
    [Google Scholar]
  8. Chany C., Lepine P., Lelong M., Le-Tan-Vinh Satge P., Virat J. 1958; Severe and fatal pneumonia in infants and young children associated with adenovirus infections. Am J Hyg 67:367–378
    [Google Scholar]
  9. Crawford-Miksza L., Schnurr D. P. 1996; Analysis of 15 adenovirus hexon proteins reveals the location and structure of seven hypervariable regions containing serotype-specific residues. J Virol 70:1836–1844
    [Google Scholar]
  10. Davison A. J., Wright K. M., Harrach B. 2000; DNA sequence of frog adenovirus. J Gen Virol 81:2431–2439
    [Google Scholar]
  11. Davison A. J., Benkő M., Harrach B. 2003; Genetic content and evolution of adenoviruses. J Gen Virol 84:2895–2908 [CrossRef]
    [Google Scholar]
  12. Defer C., Belin M.-T., Caillet-Boudin M.-L., Boulanger P. 1990; Human adenovirus-host cell interactions: comparative study with members of subgroups B and C. J Virol 64:3661–3673
    [Google Scholar]
  13. De Jong J. C., Wermenbol A. G., Verweij-Uijterwaal M. W., Slaterus K. W., Wertheim-Van Dillen P., Van Doornum G. J. J., Khoo S. H., Hierholzer J. C. 1999; Adenoviruses from human immunodeficiency virus-infected individuals, including two strains that represent new candidate serotypes Ad50 and Ad51 of species B1 and D, respectively. J Clin Microbiol 37:3940–3945
    [Google Scholar]
  14. Dudding B. A., Wagner S. C., Zeller J. A., Gmelich J. T., French G. R., Top F. H., Jr. 1972; Fatal pneumonia associated with adenovirus type 7 in three military trainees. N Engl J Med 286:1289–1292 [CrossRef]
    [Google Scholar]
  15. Erdman D. D., Xu W., Gerber S. I., Gray G. C., Schnurr D., Kajon A. E., Anderson L. J. 2002; Molecular epidemiology of adenovirus type 7 in the United States, 1966-2000. Emerg Infect Dis 8:269–277 [CrossRef]
    [Google Scholar]
  16. Farkas S. L., Benkő M., Elő P., Ursu K., Dán Á., Ahne W., Harrach B. 2002; Genomic and phylogenetic analyses of an adenovirus isolated from a corn snake ( Elaphe guttata ) imply a common origin with members of the proposed new genus Atadenovirus . J Gen Virol 83:2403–2410
    [Google Scholar]
  17. Faulkner R., Van Rooyen C. E. 1962; Adenoviruses types 3 and 5 isolated from the cerebrospinal fluid of children. Can Med Assoc J 87:1123–1125
    [Google Scholar]
  18. Frantzidou F., Pavlitou A., Mataftsi A., Dumaidi K., Georgiadis N. 2005; Molecular epidemiology of adenovirus strains isolated from patients with ocular disease in the area of Thessaloniki, Greece (1998-2002). J Med Virol 75:440–446 [CrossRef]
    [Google Scholar]
  19. Gao W., Robbins P. D., Gambotto A. 2003; Human adenovirus type 35: nucleotide sequence and vector development. Gene Ther 10:1941–1949 [CrossRef]
    [Google Scholar]
  20. Garon C. F., Berry K. W., Hierholzer J. C., Rose J. A. 1973; Mapping of base sequence heterologies between genomes from different adenovirus serotypes. Virology 54:414–426 [CrossRef]
    [Google Scholar]
  21. Golovina G. I., Zolotaryov F. N., Yurlova T. I. 1991; Sensitive analysis of genetic heterogeneity of adenovirus types 3 and 7 in the Soviet Union. J Clin Microbiol 29:2313–2321
    [Google Scholar]
  22. Green M., Mackey J. K., Wold W. S. M., Rigden P. 1979; Thirty-one human adenovirus serotypes (Ad1–Ad31) form five groups (A–E) based upon DNA genome homologies. Virology 93:481–492 [CrossRef]
    [Google Scholar]
  23. Hasson T. B., Soloway P. D., Ornelles D. A., Doerfler W., Shenk T. 1989; Adenovirus L1 52- and 55-kilodalton proteins are required for assembly of virions. J Virol 63:3612–3621
    [Google Scholar]
  24. Havenga M. J. E., Lemckert A. A. C., Ophorst O. J. A. E. & 13 other authors 2002; Exploiting the natural diversity in adenovirus tropism for therapy and prevention of disease. J Virol 76:4612–4620 [CrossRef]
    [Google Scholar]
  25. Hayes B. W., Telling G. C., Myat M. M., Williams J. F., Flint S. J. 1990; The adenovirus L4 100-kilodalton protein is necessary for efficient translation of viral late mRNA species. J Virol 64:2732–2742
    [Google Scholar]
  26. Herbert F. A., Wilkinson D., Burchak E., Morgante O. 1977; Adenovirus type 3 pneumonia causing lung damage in childhood. Can Med Assoc J 116:274–276
    [Google Scholar]
  27. Hierholzer J. C. 1995; Adenoviruses. In Diagnostic Procedures for Viral , Rickettsial, and Chlamydial Infections . , 7th edn. pp  169–188 Edited by Lennette E. H., Lennette D. A., Lennette E. T. Washington, DC: American Public Health Association;
  28. Hong J.-Y., Lee H.-J., Piedra P. A., Choi E.-H., Park K.-H., Koh Y.-Y., Kim W.-S. 2001; Lower respiratory tract infections due to adenovirus in hospitalized Korean children: epidemiology, clinical features, and prognosis. Clin Infect Dis 32:1423–1429 [CrossRef]
    [Google Scholar]
  29. Horton T. M., Tollefson A. E., Wold W. S. M., Gooding L. R. 1990; A protein serologically and functionally related to the group C E3 14,700-kilodalton protein is found in multiple adenovirus serotypes. J Virol 64:1250–1255
    [Google Scholar]
  30. Itakura S., Aoki K., Sawada H., Shinagawa M. 1990; Analysis with restriction endonucleases recognizing 4- or 5-base-pair sequences of human adenovirus type 3 isolated from ocular diseases in Sapporo, Japan. J Clin Microbiol 28:2365–2369
    [Google Scholar]
  31. Itoh N., Tanaka K., Aoki K., Hinokuma R., Nakagawa H., Takeuchi S., Uchio E., Shiao S., Ohno S. 1999; Four new genotypes of adenovirus type 3 isolated from patients with conjunctivitis in Japan. J Med Virol 59:73–77 [CrossRef]
    [Google Scholar]
  32. Jin Q. 2001; Adenovirus. In Medical Molecular Virology pp  691–710 Edited by Jin Q. Beijing: Science Press;
    [Google Scholar]
  33. Kajon A. E., Murtagh P., Garcia Franco S., Freire M. C., Weissenbacher M. C., Zorzopulos J. 1990; A new genome type of adenovirus 3 associated with severe lower acute respiratory infection in children. J Med Virol 30:73–76 [CrossRef]
    [Google Scholar]
  34. Kajon A. E., Mistchenko A. S., Videla C., Hortal M., Wadell G., Avendano L. F. 1996; Molecular epidemiology of adenovirus acute lower respiratory infections of children in the south cone of South America (1991-1994). J Med Virol 48:151–156 [CrossRef]
    [Google Scholar]
  35. Kim Y.-J., Hong J.-Y., Lee H.-J., Shin S.-H., Kim Y.-K., Inada T., Hashido M., Piedra P. A. 2003; Genome type analysis of adenovirus types 3 and 7 isolated during successive outbreaks of lower respiratory tract infections in children. J Clin Microbiol 41:4594–4599 [CrossRef]
    [Google Scholar]
  36. Kovács G. M., LaPatra S. E., D'Halluin J. C., Benkő M. 2003; Phylogenetic analysis of the hexon and protease genes of a fish adenovirus isolated from white sturgeon ( Acipenser transmontanus ) supports the proposal for a new adenovirus genus. Virus Res 98:27–34 [CrossRef]
    [Google Scholar]
  37. Kovács G. M., Davison A. J., Zakhartchouk A. N., Harrach B. 2004; Analysis of the first complete genome sequence of an Old World monkey adenovirus reveals a lineage distinct from the six human adenovirus species. J Gen Virol 85:2799–2807 [CrossRef]
    [Google Scholar]
  38. Lauer K. P., Llorente I., Blair E. & 8 other authors 2004; Natural variation among human adenoviruses: genome sequence and annotation of human adenovirus serotype 1. J Gen Virol 85:2615–2625 [CrossRef]
    [Google Scholar]
  39. Leppard K. N. 1997; E4 gene function in adenovirus, adenovirus vector and adeno-associated virus infections. J Gen Virol 78:2131–2138
    [Google Scholar]
  40. Lewis T. C., Stout J. W., Martinez P., Morray B., White L. C., Heckbert S. R., Redding G. J. 2004; Prevalence of asthma and chronic respiratory symptoms among Alaska native children. Chest 125:1665–1673 [CrossRef]
    [Google Scholar]
  41. Li Q.-G., Wadell G. 1988; Comparison of 17 genome types of adenovirus type 3 identified among strains recovered from six continents. J Clin Microbiol 26:1009–1015
    [Google Scholar]
  42. Li Q.-G., Zheng Q. J., Liu Y. H., Wadell G. 1996; Molecular epidemiology of adenovirus types 3 and 7 isolated from children with pneumonia in Beijing. J Med Virol 49:170–177 [CrossRef]
    [Google Scholar]
  43. Li L., Shimizu H., Doan L. T. P. & 8 other authors 2004; Characterizations of adenovirus type 41 isolates from children with acute gastroenteritis in Japan, Vietnam, and Korea. J Clin Microbiol 42:4032–4039 [CrossRef]
    [Google Scholar]
  44. Martone W. J., Hierholzer J. C., Keenlyside R. A., Fraser D. W., D'Angelo L. J., Winkler W. G. 1980; An outbreak of adenovirus type 3 disease at a private recreation center swimming pool. Am J Epidemiol 111:229–237
    [Google Scholar]
  45. Mei Y.-F., Lindman K., Wadell G. 1998; Two closely related adenovirus genome types with kidney or respiratory tract tropism differ in their binding to epithelial cells of various origins. Virology 240:254–266 [CrossRef]
    [Google Scholar]
  46. Mei Y.-F., Skog J., Lindman K., Wadell G. 2003; Comparative analysis of the genome organization of human adenovirus 11, a member of the human adenovirus species B, and the commonly used human adenovirus 5 vector, a member of species C. J Gen Virol 84:2061–2071 [CrossRef]
    [Google Scholar]
  47. Mizuta K., Suzuki H., Ina Y., Yazaki N., Sakamoto M., Katsushima N., Numazaki Y. 1994; Six-year longitudinal analysis of adenovirus type 3 genome types isolated in Yamagata, Japan. J Med Virol 42:198–202 [CrossRef]
    [Google Scholar]
  48. Myerowitz R. L., Stalder H., Oxman M. N., Levin M. J., Moore M., Leith J. D., Gantz N. M., Hierholzer J. C. 1975; Fatal disseminated adenovirus infection in a renal transplant recipient. Am J Med 59:591–598 [CrossRef]
    [Google Scholar]
  49. Oosterom-Dragon E. A., Ginsberg H. S. 1981; Characterization of two temperature-sensitive mutants of type 5 adenovirus with mutations in the 100,000-Dalton protein gene. J Virol 40:491–500
    [Google Scholar]
  50. Purkayastha P., Su J., Carlisle S., Tibbetts C., Seto D. 2005; Genomic and bioinformatics analysis of HAdV-7, a human adenovirus of species B1 that causes acute respiratory disease: implications for vector development in human gene therapy. Virology 332:114–129 [CrossRef]
    [Google Scholar]
  51. Roberts R. J., O'Neill K. E., Yen C. T. 1984; DNA sequences from the adenovirus 2 genome. J Biol Chem 259:13968–13975
    [Google Scholar]
  52. Roberts R. J., Akusjärvi G., Aleström P., Gelinas R. E., Gingeras T. R., Sciaky D., Pettersson U. 1986; A consensus sequence for the adenovirus-2 genome. In Adenovirus DNA: the Viral Genome and its Expression pp  1–51 Edited by Doerfler W. Boston: Martinus Nijhoff;
    [Google Scholar]
  53. Roelvink P. W., Lizonova A., Lee J. G. M., Li Y., Bergelson J. M., Finberg R. W., Brough D. E., Kovesdi I., Wickham T. J. 1998; The coxsackievirus-adenovirus receptor protein can function as a cellular attachment protein for adenovirus serotypes from subgroups A, C, D, E, and F. J Virol 72:7909–7915
    [Google Scholar]
  54. Rowe W. P., Huebner R. J., Gilmore L. K., Parrott R. H., Ward T. G. 1953; Isolation of a cytopathogenic agent from human adenoids undergoing spontaneous degeneration in tissue culture. Proc Soc Exp Biol Med 84:570–573 [CrossRef]
    [Google Scholar]
  55. Roy S., Gao G., Clawson D. S., Vandenberghe L. H., Farina S. F., Wilson J. M. 2004; Complete nucleotide sequences and genome organization of four chimpanzee adenoviruses. Virology 324:361–372 [CrossRef]
    [Google Scholar]
  56. Ryan M. A. K., Gray G. C., Smith B., McKeehan J. A., Hawksworth A. W., Malasig M. D. 2002; Large epidemic of respiratory illness due to adenovirus types 7 and 3 in healthy young adults. Clin Infect Dis 34:577–582 [CrossRef]
    [Google Scholar]
  57. Sargent K. L., Meulenbroek R. A., Parks R. J. 2004; Activation of adenoviral gene expression by protein IX is not required for efficient virus replication. J Virol 78:5032–5037 [CrossRef]
    [Google Scholar]
  58. Schwartz S., Zhang Z., Frazer K. A., Smit A., Riemer C., Bouck J., Gibbs R., Hardison R., Miller W. 2000; PipMaker – a web server for aligning two genomic DNA sequences. Genome Res 10:577–586 [CrossRef]
    [Google Scholar]
  59. Shenk T. E. 2001; Adenoviridae : the viruses and their replication. In Fields' Virology , 4th edn. pp  2265–2300 Edited by Knipe D. M., Howley P. M. Philadelphia, PA: Lippincott Williams & Wilkins;
    [Google Scholar]
  60. Shiao S., Aoki K., Isobe K., Tsuzuki W. L.-P., Itoh N., Toba K., Kobayashi N., Noguchi Y., Ohno S. 1996; Genome analysis of adenovirus type 3 isolated in Japan. J Clin Microbiol 34:413–416
    [Google Scholar]
  61. Shields A. F., Hackmann R. C., Fife K. H., Corey L., Meyers J. D. 1985; Adenovirus infections in patients undergoing bone-marrow transplantation. N Engl J Med 312:529–533 [CrossRef]
    [Google Scholar]
  62. Shinagawa M., Padmanabhan R. 1980; Comparative sequence analysis of the inverted terminal repetitions from different adenoviruses. Proc Natl Acad Sci U S A 77:3831–3835 [CrossRef]
    [Google Scholar]
  63. Shinagawa M., Matsuda A., Ishiyama T., Goto H., Sato G. 1983; A rapid and simple method for preparation of adenovirus DNA from infected cells. Microbiol Immunol 27:817–822 [CrossRef]
    [Google Scholar]
  64. Similä S., Jouppila R., Salmi A., Pohjonen R. 1970; Encephaloningitis in children associated with an adenovirus type 7 epidemic. Acta Paediatr Scand 59:310–316 [CrossRef]
    [Google Scholar]
  65. Sirena D., Lilienfeld B., Eisenhut M. & 8 other authors 2004; The human membrane cofactor CD46 is a receptor for species B adenovirus serotype 3. J Virol 78:4454–4462 [CrossRef]
    [Google Scholar]
  66. Stone D., Furthmann A., Sandig V., Lieber A. 2003; The complete nucleotide sequence, genome organization, and origin of human adenovirus type 11. Virology 309:152–165 [CrossRef]
    [Google Scholar]
  67. Temperley S. M., Hay R. T. 1992; Recognition of the adenovirus type 2 origin of DNA replication by the virally encoded DNA polymerase and preterminal proteins. EMBO J 11:761–768
    [Google Scholar]
  68. Tolun A., Aleström P., Pettersson U. 1979; Sequence of inverted terminal repetitions from different adenoviruses: demonstration of conserved sequences and homology between SA7 termini and SV40 DNA. Cell 17:705–713 [CrossRef]
    [Google Scholar]
  69. Van der Veen J. 1963; The role of adenoviruses in respiratory disease. Am Rev Respir Dis 88:167–180
    [Google Scholar]
  70. Vogels R., Zuijdgeest D., van Rijnsoever R. & 20 other authors 2003; Replication-deficient human adenovirus type 35 vectors for gene transfer and vaccination: efficient human cell infection and bypass of preexisting adenovirus immunity. J Virol 77:8263–8271 [CrossRef]
    [Google Scholar]
  71. Wadell G. 1979; Classification of human adenoviruses by SDS-polyacrylamide gel electrophoresis of structural polypeptides. Intervirology 11:47–57 [CrossRef]
    [Google Scholar]
  72. Wadell G. 1984; Molecular epidemiology of human adenoviruses. Curr Top Microbiol Immunol 110:191–220
    [Google Scholar]
  73. Wadell G., Hammarskjold M. L., Winberg G., Varsanyi T. M., Sundell G. 1980; Genetic variability of adenoviruses. Ann N Y Acad Sci 354:16–42 [CrossRef]
    [Google Scholar]
  74. Wickham T. J., Mathias P., Cheresh D. A., Nemerow G. R. 1993; Integrins α v β 3 and α v β 5 promote adenovirus internalization but not virus attachment. Cell 73:309–319 [CrossRef]
    [Google Scholar]
  75. Wold W. S., Mackey J. K., Rigden P., Green M. 1979; Analysis of human cancer DNA's for DNA sequence of human adenovirus serotypes 3, 7, 11, 14, 16, and 21 in group B1. Cancer Res 39:3479–3484
    [Google Scholar]
  76. Yeh R.-F., Lim L. P., Burge C. B. 2001; Computational inference of homologous gene structures in the human genome. Genome Res 11:803–816 [CrossRef]
    [Google Scholar]
  77. Yew P. R., Liu X., Berk A. J. 1994; Adenovirus E1B oncoprotein tethers a transcriptional repression domain to p53. Genes Dev 8:190–202 [CrossRef]
    [Google Scholar]
  78. Zhang W., Low J. A., Christensen J. B., Imperiale M. J. 2001; Role for the adenovirus IVa2 protein in packaging of viral DNA. J Virol 75:10446–10454 [CrossRef]
    [Google Scholar]
  79. Zhu B., Su X., Gong S., Bai P., Zhou R., Liu X. 2005; Pathogenic research of human adenovirus type 3 causing acute respiratory disease in children. J Mod Clin Med Bioeng 11:134–135
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.81515-0
Loading
/content/journal/jgv/10.1099/vir.0.81515-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error