1887

Abstract

Extracellular proteases that are expressed in primary and secondary foci of viral infection are potentially important mediators of infectious inflammatory processes. For some viruses, such as influenza virus and rotaviruses, proteases such as trypsin enhance infectivity by a direct proteolytic effect on some virion proteins. By using an model of herpesvirus infection, the possibility that proteases modulate the viral cycle through signalling delivered to the infected cell was investigated. It is reported that exposure of pseudorabies virus-infected cells to trypsin increased virus production. Moreover, this treatment induced synergistic and sustained activation of the extracellular signal-regulated kinase (ERK) 1/2 signalling pathway, which appeared to be necessary for this increased viral production. These results suggest that herpesviruses could take advantage of the inflammatory context and particularly of the presence of proteases to increase their replication. Thus, these data point to a potentially important role of extracellular proteases in herpesvirus infection.

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2006-05-01
2024-03-28
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References

  1. Avota E., Müller N., Klett M., Schneider-Schaulies S. 2004; Measles virus interacts with and alters signal transduction in T-cell lipid rafts. J Virol 78:9552–9559 [CrossRef]
    [Google Scholar]
  2. Brown J. K., Jones C. A., Rooney L. A., Caughey G. H. 2001; Mast cell tryptase activates extracellular-regulated kinases (p44/p42) in airway smooth-muscle cells: importance of proteolytic events, time course, and role in mediating mitogenesis. Am J Respir Cell Mol Biol 24:146–154 [CrossRef]
    [Google Scholar]
  3. Darmoul D., Gratio V., Devaud H., Laburthe M. 2004; Protease-activated receptor 2 in colon cancer: trypsin-induced MAPK phosphorylation and cell proliferation are mediated by epidermal growth factor receptor transactivation. J Biol Chem 279:20927–20934 [CrossRef]
    [Google Scholar]
  4. DeFea K. A., Zalevsky J., Thoma M. S., Déry O., Mullins R. D., Bunnett N. W. 2000; β -Arrestin-dependent endocytosis of proteinase-activated receptor 2 is required for intracellular targeting of activated ERK1/2. J Cell Biol 148:1267–1281 [CrossRef]
    [Google Scholar]
  5. Dykstra M., Cherukuri A., Sohn H. W., Tzeng S.-J., Pierce S. K. 2003; Location is everything: lipid rafts and immune cell signaling. Annu Rev Immunol 21:457–481 [CrossRef]
    [Google Scholar]
  6. Eloit M., Fargeaud D., L'Haridon R., Toma B. 1988; Identification of the pseudorabies virus glycoprotein gp50 as a major target of neutralizing antibodies. Arch Virol 99:45–56 [CrossRef]
    [Google Scholar]
  7. Favoreel H. W., Mettenleiter T. C., Nauwynck H. J. 2004; Copatching and lipid raft association of different viral glycoproteins expressed on the surfaces of pseudorabies virus-infected cells. J Virol 78:5279–5287 [CrossRef]
    [Google Scholar]
  8. Klupp B. G., Hengartner C. J., Mettenleiter T. C., Enquist L. W. 2004; Complete, annotated sequence of the pseudorabies virus genome. J Virol 78:424–440 [CrossRef]
    [Google Scholar]
  9. Luo H., Yanagawa B., Zhang J. & 7 other authors 2002; Coxsackievirus B3 replication is reduced by inhibition of the extracellular signal-regulated kinase (ERK) signaling pathway. J Virol 76:3365–3373 [CrossRef]
    [Google Scholar]
  10. Mañes S., del Real G., Martínez-A C. 2003; Pathogens: raft hijackers. Nat Rev Immunol 3:557–568 [CrossRef]
    [Google Scholar]
  11. Mettenleiter T. C. 2002; Herpesvirus assembly and egress. J Virol 76:1537–1547 [CrossRef]
    [Google Scholar]
  12. Ossovskaya V. S., Bunnett N. W. 2004; Protease-activated receptors: contribution to physiology and disease. Physiol Rev 84:579–621 [CrossRef]
    [Google Scholar]
  13. Perkins D., Pereira E. F. R., Aurelian L. 2003; The herpes simplex virus type 2 R1 protein kinase (ICP10 PK) functions as a dominant regulator of apoptosis in hippocampal neurons involving activation of the ERK survival pathway and upregulation of the antiapoptotic protein Bag-1. J Virol 77:1292–1305 [CrossRef]
    [Google Scholar]
  14. Pleschka S., Wolff T., Ehrhardt C., Hobom G., Planz O., Rapp U. R., Ludwig S. 2001; Influenza virus propagation is impaired by inhibition of the Raf/MEK/ERK signalling cascade. Nat Cell Biol 3:301–305 [CrossRef]
    [Google Scholar]
  15. Ray N., Enquist L. W. 2004; Transcriptional response of a common permissive cell type to infection by two diverse alphaherpesviruses. J Virol 78:3489–3501 [CrossRef]
    [Google Scholar]
  16. Riteau B., Barber D. F., Long E. O. 2003; Vav1 phosphorylation is induced by β 2 integrin engagement on natural killer cells upstream of actin cytoskeleton and lipid raft reorganization. J Exp Med 198:469–474 [CrossRef]
    [Google Scholar]
  17. Roy S., Luetterforst R., Harding A., Apolloni A., Etheridge M., Stang E., Rolls B., Hancock J. F., Parton R. G. 1999; Dominant-negative caveolin inhibits H-Ras function by disrupting cholesterol-rich plasma membrane domains. Nat Cell Biol 1:98–105 [CrossRef]
    [Google Scholar]
  18. Stark G. R., Kerr I. M., Williams B. R. G., Silverman R. H., Schreiber R. D. 1998; How cells respond to interferons. Annu Rev Biochem 67:227–264 [CrossRef]
    [Google Scholar]
  19. Steinhoff M., Buddenkotte J., Shpacovitch V., Rattenholl A., Moormann C., Vergnolle N., Luger T. A., Hollenberg M. D. 2005; Proteinase-activated receptors: transducers of proteinase-mediated signaling in inflammation and immune response. Endocr Rev 26:1–43
    [Google Scholar]
  20. Zhirnov O. P., Ikizler M. R., Wright P. F. 2002; Cleavage of influenza A virus hemagglutinin in human respiratory epithelium is cell associated and sensitive to exogenous antiproteases. J Virol 76:8682–8689 [CrossRef]
    [Google Scholar]
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