RT Journal Article SR Electronic(1) A1 Buffa, Viviana A1 Negri, Donatella R. M. A1 Leone, Pasqualina A1 Bona, Roberta A1 Borghi, Martina A1 Bacigalupo, Ilaria A1 Carlei, Davide A1 Sgadari, Cecilia A1 Ensoli, Barbara A1 Cara, AndreaYR 2006 T1 A single administration of lentiviral vectors expressing either full-length human immunodeficiency virus 1 (HIV-1)HXB2 Rev/Env or codon-optimized HIV-1JR-FL gp120 generates durable immune responses in mice JF Journal of General Virology, VO 87 IS 6 SP 1625 OP 1634 DO https://doi.org/10.1099/vir.0.81706-0 PB Microbiology Society, SN 1465-2099, AB Genetic immunization using viral vectors provides an effective means to elicit antigen-specific cellular immune responses. Several viral vectors have proven efficacious in inducing immune responses after direct injection in vivo. Among them, recombinant, self-inactivating lentiviral vectors are very attractive delivery systems, as they are able to efficiently transduce into and express foreign genes in a wide variety of mammalian cells. A self-inactivating lentiviral vector was evaluated for the delivery of human immunodeficiency virus 1 (HIV-1) envelope sequences in mice in order to elicit specific immune responses. With this aim, BALB/c mice were immunized with a single injection of self-inactivating lentiviral vectors carrying either the full-length HIV-1HXB2 Rev/Env (TY2-IIIBEnv) or the codon-optimized HIV-1JR-FL gp120 (TY2-JREnv) coding sequence. Both vectors were able to elicit specific cellular responses efficiently, as measured by gamma interferon ELISPOT and chromium-release assays, upon in vitro stimulation of splenocytes from BALB/c immunized mice. However, only the TY2-JREnv-immunized mice were able to elicit specific humoral responses, measured as anti-gp120 antibody production. These data provide the first evidence that a single, direct, in vivo administration of a lentiviral vector encoding a viral gene might represent a useful strategy for vaccine development., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.81706-0