RT Journal Article SR Electronic(1) A1 Mase, Masaji A1 Tanimura, Nobuhiko A1 Imada, Tadao A1 Okamatsu, Masatoshi A1 Tsukamoto, Kenji A1 Yamaguchi, ShigeoYR 2006 T1 Recent H5N1 avian Influenza A virus increases rapidly in virulence to mice after a single passage in mice JF Journal of General Virology, VO 87 IS 12 SP 3655 OP 3659 DO https://doi.org/10.1099/vir.0.81843-0 PB Microbiology Society, SN 1465-2099, AB To evaluate the potential pathogenicity to mammals of the recent H5N1 avian Influenza A virus, viruses recovered from dead mice infected with A/chicken/Yamaguchi/7/2004 isolated in Japan were examined. All recovered viruses from the brains of dead mice infected with this strain (without any prior adaptation to mice) had substituted the amino acid at position 627 of the PB2 protein from glutamic acid to lysine. Their mouse lethality had increased by approximately 5×104 times over that of the original virus. Histopathological analysis reinforced the finding that these variants caused more rapid and severe damage to mice than the original virus. This revealed that it might be useful to characterize the recovered virus to assess its potential pathogenicity to mammals., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.81843-0