RT Journal Article SR Electronic(1) A1 Beard, Philippa M. A1 Froggatt, Graham C. A1 Smith, Geoffrey L.YR 2006 T1 Vaccinia virus kelch protein A55 is a 64 kDa intracellular factor that affects virus-induced cytopathic effect and the outcome of infection in a murine intradermal model JF Journal of General Virology, VO 87 IS 6 SP 1521 OP 1529 DO https://doi.org/10.1099/vir.0.81854-0 PB Microbiology Society, SN 1465-2099, AB The vaccinia virus (VACV) protein A55 is a BTB/kelch protein with a broad-complex, tramtrack and bric-a-brac (BTB) domain in the N-terminal region and five kelch repeats in the C-terminal half. The BTB/kelch subgroup of the kelch superfamily of proteins has been associated with a wide variety of functions including regulation of the cytoskeleton. VACV contains three genes predicted to encode BTB/kelch proteins: A55R, F3L and C2L. The A55R gene product has been identified as an intracellular protein of 64 kDa that is expressed late in infection. A VACV strain lacking 93.6 % of the A55R open reading frame (vΔA55) was constructed and found to have an unaltered growth rate in vivo but a different plaque morphology and cytopathic effect, as well as reduced development of VACV-induced Ca2+-independent cell/extracellular matrix adhesion. In a murine intradermal model of VACV infection, a virus lacking the A55R gene induced larger lesions than wild-type and revertant control viruses., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.81854-0