1887

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Volume 87, Issue 12

infection and propagation of wild-type in human T lymphocytes Free

Abstract

While exploring previous findings that treatment of lymphoid cells from (HCV)-infected individuals with T cell-stimulating mitogens augments detection of the residing virus, an HCV replication system was established, in which mitogen-induced T cell-enriched cultures served as HCV targets and the derived T cells multiplied virus during repeated serial passage. HCV replication was ascertained by detecting HCV RNA positive and negative strands, HCV NS5a and E2 proteins, release of HCV virions and nucleocapsids (confirmed by immunoelectron microscopy) and infection of mitogen-induced T cells prepared from healthy donors. Further, affinity-purified normal human T lymphocytes were also susceptible to HCV infection and HCV replication was detected in pure T cells isolated from a patient with chronic hepatitis C. These results document that T cells can support propagation of HCV both and . The infection system established offers a valuable tool for studies on the entire cycle of HCV replication, virus cytopathogenicity and evaluation of antiviral agents against wild-type HCV in the natural host-cell milieu.

  • Received:
  • Accepted:
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2006-12-01
2024-04-25
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De novo infection and propagation of wild-type Hepatitis C virus in human T lymphocytes in vitro
J. Gen. Virol. 87, 3577 (2006); https://doi.org/10.1099/vir.0.81868-0
/content/journal/jgv/10.1099/vir.0.81868-0
/content/journal/jgv/10.1099/vir.0.81868-0
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