1887

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Volume 87, Issue 8

Identification of an RNA-silencing suppressor in the genome of Free

Abstract

Higher plants use post-transcriptional gene silencing (PTGS), an RNA-degradation system, as a defence mechanism against viral infections. To counteract this, plant viruses encode and express PTGS suppressor proteins. Four of the five proteins encoded by the (GVA) genome were screened using a green fluorescent protein (GFP)-based transient expression assay, and the expression product of ORF5 (protein p10) was identified as a suppressor of silencing. ORF5 p10 suppressed local and systemic silencing induced by a transiently expressed single-stranded sense RNA. This protein was active towards both a transgene and exogenous GFP mRNAs. Ectopic expression of GVA-ORF5 by a vector enhanced symptom severity. The findings that p10 markedly reduces the levels of small interfering RNAs (siRNAs) and that the recombinant protein is able to bind single-stranded and double-stranded forms of siRNAs and microRNAs, suggest the existence of a potential mechanism of suppression based on RNA sequestering.

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2006-08-01
2024-04-25
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Identification of an RNA-silencing suppressor in the genome of Grapevine virus A
J. Gen. Virol. 87, 2387 (2006); https://doi.org/10.1099/vir.0.81893-0
/content/journal/jgv/10.1099/vir.0.81893-0
/content/journal/jgv/10.1099/vir.0.81893-0
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