1887

Abstract

By using degenerate primers deduced from conserved patterns in the flavivirus polymerase gene, a novel RNA virus was discovered in ticks sampled from members of the family Bovidae in Senegal. It was named Ngoye virus (NGOV) after the location from which it was isolated. Viral particles could be observed by electron microscopy, but isolation in vertebrate or invertebrate cell lines or by intracerebral infection of newborn mice remained unsuccessful. This is atypical of recognized arboviruses. The characterization of 4176 nt of the non-structural genes revealed that NGOV is a novel flavivirus species. It forms a distinct phylogenetic lineage related distantly to previously identified members of the genus . Analysis of genetic data suggested that the processing of the NGOV polyprotein and the organization of its replication complex are similar to those of flaviviruses. Together with other recent data, these findings suggest that a large number of viruses related distantly to ‘classical’ arthropod-borne flaviviruses remain to be discovered.

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2006-11-01
2024-04-23
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References

  1. Bakonyi T., Hubálek Z., Rudolf I., Nowotny N. 2005; Novel flavivirus or new lineage of West Nile virus, central Europe. Emerg Infect Dis 11:225–231 [CrossRef]
    [Google Scholar]
  2. Bendtsen J. D., Nielsen H., von Heijne G., Brunak S. 2004; Improved prediction of signal peptides: SignalP 3.0. J Mol Biol 340:783–795 [CrossRef]
    [Google Scholar]
  3. Billoir F., de Chesse R., Tolou H., de Micco P., Gould E. A., de Lamballerie X. 2000; Phylogeny of the genus Flavivirus using complete coding sequences of arthropod-borne viruses and viruses with no known vector. J Gen Virol 81:781–790
    [Google Scholar]
  4. Calisher C. H., Gould E. A. 2003; Taxonomy of the virus family Flaviviridae . Adv Virus Res 59:1–19
    [Google Scholar]
  5. Cammisa-Parks H., Cisar L. A., Kane A., Stollar V. 1992; The complete nucleotide sequence of cell fusing agent (CFA): homology between the nonstructural proteins encoded by CFA and the nonstructural proteins encoded by arthropod-borne flaviviruses. Virology 189:511–524 [CrossRef]
    [Google Scholar]
  6. Cook S., Holmes E. C. 2006; A multigene analysis of the phylogenetic relationships among the flaviviruses (family: Flaviviridae ) and the evolution of vector transmission. Arch Virol 151:309–325 [CrossRef]
    [Google Scholar]
  7. Cook S., Bennett S. N., Holmes E. C., De Chesse R., Moureau G., de Lamballerie X. 2006; Isolation of a new strain of the flavivirus cell fusing agent virus in a natural mosquito population from Puerto Rico. J Gen Virol 87:735–748 [CrossRef]
    [Google Scholar]
  8. Crabtree M. B., Sang R. C., Stollar V., Dunster L. M., Miller B. R. 2003; Genetic and phenotypic characterization of the newly described insect flavivirus, Kamiti River virus. Arch Virol 148:1095–1118 [CrossRef]
    [Google Scholar]
  9. Crochu S., Cook S., Attoui H., Charrel R. N., de Chesse R., Belhouchet M., Lemasson J.-J., De Micco P., de Lamballerie X. 2004; Sequences of flavivirus-related RNA viruses persist in DNA form integrated in the genome of Aedes spp. mosquitoes. J Gen Virol 85:1971–1980 [CrossRef]
    [Google Scholar]
  10. de Lamballerie X., Crochu S., Billoir F., Neyts J., de Micco P., Holmes E. C., Gould E. A. 2002; Genome sequence analysis of Tamana bat virus and its relationship with the genus Flavivirus . J Gen Virol 83:2443–2454
    [Google Scholar]
  11. Edgar R. C. 2004; muscle: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res 32:1792–1797 [CrossRef]
    [Google Scholar]
  12. Gaunt M. W., Sall A. A., de Lamballerie X., Falconar A. K. I., Dzhivanian T. I., Gould E. A. 2001; Phylogenetic relationships of flaviviruses correlate with their epidemiology, disease association and biogeography. J Gen Virol 82:1867–1876
    [Google Scholar]
  13. Gritsun T. S., Lashkevich V. A., Gould E. A. 2003; Tick-borne encephalitis. Antiviral Res 57:129–146 [CrossRef]
    [Google Scholar]
  14. Halstead S. B., Jacobson J. 2003; Japanese encephalitis. Adv Virus Res 61:103–138
    [Google Scholar]
  15. Komar N. 2003; West Nile virus: epidemiology and ecology in North America. Adv Virus Res 61:185–234
    [Google Scholar]
  16. Kono Y., Tsukamoto K., Abd Hamid M. & 8 other authors 2000; Encephalitis and retarded growth of chicks caused by Sitiawan virus, a new isolate belonging to the genus Flavivirus . Am J Trop Med Hyg 63:94–101
    [Google Scholar]
  17. Kuno G., Chang G.-J. J., Tsuchiya K. R., Karabatsos N., Cropp C. B. 1998; Phylogeny of the genus Flavivirus . J Virol 72:73–83
    [Google Scholar]
  18. Nisbet D. J., Lee K. J., van den Hurk A. F., Johansen C. A., Kuno G., Chang G.-J. J., Mackenzie J. S., Ritchie S. A., Hall R. A. 2005; Identification of new flaviviruses in the Kokobera virus complex. J Gen Virol 86:121–124 [CrossRef]
    [Google Scholar]
  19. Price J. L. 1978; Isolation of Rio Bravo and a hitherto undescribed agent, Tamana bat virus, from insectivorous bats in Trinidad, with serological evidence of infection in bats and man. Am J Trop Med Hyg 27:153–161
    [Google Scholar]
  20. Pybus O. G., Rambaut A., Holmes E. C., Harvey P. H. 2002; New inferences from tree shape: numbers of missing taxa and population growth rates. Syst Biol 51:881–888 [CrossRef]
    [Google Scholar]
  21. Strimmer K., von Haeseler A. 1996; Quartet puzzling: a quartet maximum-likelihood method for reconstructing tree topologies. Mol Biol Evol 13:964–969 [CrossRef]
    [Google Scholar]
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