1887

Abstract

The cucumber mosaic virus (CMV) replication complex has previously been shown to associate with cellular membranes. However, it remains unknown whether any host factors participate in this process. In this study, five groups of tonoplast intrinsic protein (TIP) genes were isolated and the proteins they encoded were evaluated with regard to their interactions with CMV proteins. TIP1 and TIP2 were found to interact with the CMV 1a protein in the Sos recruitment system, whereas no interactions with the other three TIP subgroups were observed in this assay. The interaction of CMV 1a with the TIP1 and TIP2 proteins was confirmed via co-immunoprecipitation assays. Additionally, CMV 1a co-localized with TIP1 and TIP2 in transfected protoplasts. The findings of this study suggest that members of two TIP subfamilies might affect CMV replication via interaction with CMV 1a in the tonoplasts.

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2006-11-01
2024-04-19
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References

  1. Alexandersson E., Fraysse L., Sjovall-Larsen S., Gustavsson S., Fellert M., Karlsson M., Johanson U., Kjellbom P. 2005; Whole gene family expression and drought stress regulation of aquaporins. Plant Mol Biol 59:469–484 [CrossRef]
    [Google Scholar]
  2. Barton D. J., Flanegan J. B. 1993; Coupled translation and replication of poliovirus RNA in vitro : synthesis of functional 3D polymerase and infectious virus. J Virol 67:822–831
    [Google Scholar]
  3. Bienz K., Egger D., Pasamontes L. 1987; Association of polioviral proteins of the P2 genomic region with the viral replication complex and virus-induced membrane synthesis as visualized by electron microscopic immunocytochemistry and autoradiography. Virology 160:220–226 [CrossRef]
    [Google Scholar]
  4. Buck K. W. 1996; Comparison of the replication of positive-stranded RNA viruses of plants and animals. Adv Virus Res 47:159–251
    [Google Scholar]
  5. Chrispeels M. J., Maurel C. 1994; Aquaporins: the molecular basis of facilitated water movement through living plant cells. Plant Physiol 105:9–13 [CrossRef]
    [Google Scholar]
  6. Cillo F., Roberts I. M., Palukaitis P. 2002; In situ localization and tissue distribution of the replication-associated proteins of Cucumber mosaic virus in tobacco and cucumber. J Virol 76:10654–10664 [CrossRef]
    [Google Scholar]
  7. Froshauer S., Kartenbeck J., Helenius A. 1988; Alphavirus RNA replicase is located on the cytoplasmic surface of endosomes and lysosomes. J Cell Biol 107:2075–2086 [CrossRef]
    [Google Scholar]
  8. Hayes R. J., Buck K. W. 1990; Complete replication of a eukaryotic virus RNA in vitro by a purified RNA dependent RNA polymerase. Cell 63:363–368 [CrossRef]
    [Google Scholar]
  9. Hugle T., Fehrmann F., Bieck E., Kohara M., Krausslich H. G., Rice C. M., Blum H. E., Moradpour D. 2001; The hepatitis C virus nonstructural protein 4B is an integral endoplasmic reticulum membrane protein. Virology 284:70–81 [CrossRef]
    [Google Scholar]
  10. Jauh G. Y., Fischer A. M., Grimes H. D., Ryan C. A. Jr, Rogers J. C. 1998; δ -Tonoplast intrinsic protein defines unique plant vacuole functions. Proc Natl Acad Sci U S A 95:12995–12999 [CrossRef]
    [Google Scholar]
  11. Jauh G. Y., Phillips T. E., Rogers J. C. 1999; Tonoplast intrinsic protein isoforms as markers for vacuolar functions. Plant Cell 11:1867–1882 [CrossRef]
    [Google Scholar]
  12. Jiang L., Phillips T. E., Rogers S. W., Rogers J. C. 2000; Biogenesis of the protein storage vacuole crystalloid. J Cell Biol 150:755–770 [CrossRef]
    [Google Scholar]
  13. Jin J. B., Kim Y. A., Kim S. J., Lee S. H., Kim D. H., Cheong G. W., Hwang I. 2001; A new dynamin-like protein, ADL6, is involved in trafficking from the trans-Golgi network to the central vacuole in Arabidopsis . Plant Cell 13:1511–1526 [CrossRef]
    [Google Scholar]
  14. Johanson U., Karlsson M., Johansson I., Gustavsson S., Sjovall S., Fraysse L., Weig A. R., Kjellbom P. 2001; The complete set of genes encoding major intrinsic proteins in Arabidopsis provides a framework for a new nomenclature for major intrinsic proteins in plants. Plant Physiol 126:1358–1369 [CrossRef]
    [Google Scholar]
  15. Kleizen B., van Vlijmen T., de Jonge H. R., Braakman I. 2005; Folding of CFTR is predominantly cotranslational. Mol Cell 20:277–287 [CrossRef]
    [Google Scholar]
  16. Kujala P., Ahola T., Ehsani N., Auvinen P., Vihinen H., Kaariainen L. 1999; Intracellular distribution of rubella virus nonstructural protein P150. J Virol 73:7805–7811
    [Google Scholar]
  17. Maurel C. 1997; Aquaporins and water permeability of plant membranes. Annu Rev Plant Physiol Plant Mol Biol 48:399–429 [CrossRef]
    [Google Scholar]
  18. Miller D. J., Ahlquist P. 2002; Flock house virus RNA polymerase is a transmembrane protein with amino-terminal sequences sufficient for mitochondrial localization and membrane insertion. J Virol 76:9856–9867 [CrossRef]
    [Google Scholar]
  19. Molla A., Paul A. V., Wimmer E. 1993; Effects of temperature and lipophilic agents on poliovirus formation and RNA synthesis in a cell-free system. J Virol 67:5932–5938
    [Google Scholar]
  20. Moriyasu Y., Hattori M., Jauh G. Y., Rogers J. C. 2003; Alpha tonoplast intrinsic protein is specifically associated with vacuole membrane involved in an autophagic process. Plant Cell Physiol 44:795–802 [CrossRef]
    [Google Scholar]
  21. Nakai K., Kanehisa M. 1992; A knowledge base for predicting protein localization sites in eukaryotic cells. Genomics 14:897–911 [CrossRef]
    [Google Scholar]
  22. Pedersen K. W., van der Meer Y., Roos N., Snijder E. J. 1999; Open reading frame 1a-encoded subunits of the arterivirus replicase induce endoplasmic reticulum-derived double-membrane vesicles which carry the viral replication complex. J Virol 73:2016–2026
    [Google Scholar]
  23. Saito C., Ueda T., Abe H., Wada Y., Kuroiwa T., Hisada A., Furuya M., Nakano A. 2002; A complex and mobile structure forms a distinct subregion within the continuous vacuolar membrane in young cotyledons of Arabidopsis . Plant J 29:245–255 [CrossRef]
    [Google Scholar]
  24. Schlegel A., Giddings T. H. Jr, Ladinsky M. S., Kirkegaard K. 1996; Cellular origin and ultrastructure of membranes induced during poliovirus infection. J Virol 70:6576–6588
    [Google Scholar]
  25. Suhy D. A., Giddings T. H. Jr, Kirkegaard K. 2000; Remodeling the endoplasmic reticulum by poliovirus infection and by individual viral proteins: an autophagy-like origin for virus-induced vesicles. J Virol 74:8953–8965 [CrossRef]
    [Google Scholar]
  26. Tereba A., Lai M. M. 1982; Cell oncogenes are located on the large microchromosomes in chicken cells. Virology 30:654–657
    [Google Scholar]
  27. Thaminy S., Auerbach D., Arnoldo A., Stagljar I. 2003; Identification of novel ErbB3-interacting factors using the split-ubiquitin membrane yeast two-hybrid system. Genome Res 13:1744–1753 [CrossRef]
    [Google Scholar]
  28. Tsujimoto Y., Numaga T., Ohshima K., Yano M., Ohsawa R., Goto D., Naito S., Ishikawa M. 2003; Arabidopsis tobamovirus multiplication (TOM)2 locus encodes a transmembrane protein that interacts with TOM1. EMBO J 22:335–343 [CrossRef]
    [Google Scholar]
  29. Ueda T., Yamaguchi M., Uchimiya H., Nakano A. 2001; Ara6, a plant-unique novel type Rab GTPase, functions in the endocytic pathway of Arabidopsis thaliana . EMBO J 20:4730–4741 [CrossRef]
    [Google Scholar]
  30. van der Meer Y., van Tol H., Locker J. K., Snijder E. J. 1998; ORF1a-encoded replicase subunits are involved in the membrane association of the arterivirus replication complex. J Virol 72:6689–6698
    [Google Scholar]
  31. Vida T. A., Emr S. D. 1995; A new vital stain for visualizing vacuolar membrane dynamics and endocytosis in yeast. J Cell Biol 128:779–792 [CrossRef]
    [Google Scholar]
  32. Wu S. X., Ahlquist P., Kaesberg P. 1992; Active complete in vitro replication of nodavirus RNA requires glycerophospholipid. Proc Natl Acad Sci U S A 89:11136–11140 [CrossRef]
    [Google Scholar]
  33. Yamanaka T., Imai T., Satoh R. & 7 other authors 2002; Complete inhibition of tobamovirus multiplication by simultaneous mutations in two homologous host genes. J Virol 76:2491–2497 [CrossRef]
    [Google Scholar]
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