1887

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Volume 88, Issue 4

Molecular analysis of bovine spongiform encephalopathy infection by cDNA arrays Free

Abstract

Here, the first cDNA array analysis of differential gene expression in bovine spongiform encephalopathy (BSE) is reported, using a spotted cDNA array platform representing nearly 17 000 mouse genes. Array analysis identified 296 gene candidates for differential expression in brain tissue from VM mice in late-stage infection with the 301V strain of BSE, compared with brain tissue from normal, age-matched VM mice. Real-time PCR confirmed differential expression of 25 of 31 genes analysed. Some of the genes identified by array analysis as being expressed differentially are associated with ubiquitin/proteasome function, lysosomal function, molecular chaperoning of protein folding or apoptosis. Other genes are involved in calcium ion binding/homeostasis, zinc ion binding/homeostasis or regulation of transcription. Principal-component analysis shows that the global gene-expression profiles of the BSE-infected samples have gene-expression signatures that are markedly different from, and completely non-overlapping with, those obtained from the normal controls.

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2007-04-01
2024-04-20
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References

  1. Artal-Sanz M., Tavernarakis N. 2005; Proteolytic mechanisms in necrotic cell death and neurodegeneration. FEBS Lett 579:3287–3296 [CrossRef]
     [Google Scholar]
  2. Baker C. A., Manuelidis L. 2003; Unique inflammatory RNA profiles of microglia in Creutzfeldt–Jakob disease. Proc Natl Acad Sci U S A 100:675–679 [CrossRef]
     [Google Scholar]
  3. Baker C. A., Lu Z. Y., Manuelidis L. 2004; Early induction of interferon-responsive mRNAs in Creutzfeldt–Jakob disease. J Neurovirol 10:29–40
     [Google Scholar]
  4. Booth S., Bowman C., Baumgartner R., Sorensen G., Robertson C., Coulthart M., Phillipson C., Somorjai R. L. 2004a; Identification of central nervous system genes involved in the host response to the scrapie agent during preclinical and clinical infection. J Gen Virol 85:3459–3471 [CrossRef]
     [Google Scholar]
  5. Booth S., Bowman C., Baumgartner R., Dolenko B., Sorensen G., Robertson C., Coulthart M., Phillipson C., Somorjai R. 2004b; Molecular classification of scrapie strains in mice using gene expression profiling. Biochem Biophys Res Commun 325:1339–1345 [CrossRef]
     [Google Scholar]
  6. Braunewell K. H. 2005; The darker side of Ca2+ signaling by neuronal Ca2+-sensor proteins: from Alzheimer's disease to cancer. Trends Pharmacol Sci 26:345–351 [CrossRef]
     [Google Scholar]
  7. Brini M. 2003; Ca2+ signalling in mitochondria: mechanism and role in physiology and pathology. Cell Calcium 34:399–405 [CrossRef]
     [Google Scholar]
  8. Brown A. R., Webb J., Rebus S., Williams A., Fazakerley J. K. 2004; Identification of up-regulated genes by array analysis in scrapie-infected mouse brains. Neuropathol Appl Neurobiol 30:555–567 [CrossRef]
     [Google Scholar]
  9. Bruce M. E., Will R. G., Ironside J. W., McConnell I., Drummond D., Suttie A., McCardle L., Chree A., Hope J. other authors 1997; Transmissions to mice indicate that 'new variant' CJD is caused by the BSE agent. Nature 389:498–501 [CrossRef]
     [Google Scholar]
  10. Cheadle C., Vawter M. P., Freed W. J., Becker K. G. 2003; Analysis of microarray data using Z score transformation. J Mol Diagn 5:73–81 [CrossRef]
     [Google Scholar]
  11. Ciechanover A., Brundin P. 2003; The ubiquitin proteasome system in neurodegenerative diseases: sometimes the chicken, sometimes the egg. Neuron 40:427–446 [CrossRef]
     [Google Scholar]
  12. Dandoy-Dron F., Guillo F., Benboudjema L., Deslys J. P., Lasmezas C., Dormont D., Tovey M. G., Dron M. 1998; Gene expression in scrapie. Cloning of a new scrapie-responsive gene and the identification of increased levels of seven other mRNA transcripts. J Biol Chem 273:7691–7697 [CrossRef]
     [Google Scholar]
  13. Diedrich J., Wietgrefe S., Zupancic M., Staskus K., Retzel E., Haase A. T., Race R. 1987; The molecular pathogenesis of astrogliosis in scrapie and Alzheimer's disease. Microb Pathog 2:435–442 [CrossRef]
     [Google Scholar]
  14. Diedrich J. F., Carp R. I., Haase A. T. 1993; Increased expression of heat shock protein, transferrin, and beta 2-microglobulin in astrocytes during scrapie. Microb Pathog 15:1–6 [CrossRef]
     [Google Scholar]
  15. Doh-ura K., Perryman S., Race R., Chesebro B. 1995; Identification of differentially expressed genes in scrapie-infected mouse neuroblastoma cells. Microb Pathog 18:1–9 [CrossRef]
     [Google Scholar]
  16. Dudoit S., Yang Y. H., Callow M. J., Speed T. P. 2002; Statistical methods for identifying differentially expressed genes in replicated cDNA microarray experiments. Statist Sinica 12:111–139
     [Google Scholar]
  17. Duguid J. R., Dinauer M. C. 1990; Library subtraction of in vitro cDNA libraries to identify differentially expressed genes in scrapie infection. Nucleic Acids Res 18:2789–2792 [CrossRef]
     [Google Scholar]
  18. Duguid J. R., Rohwer R. G., Seed B. 1988; Isolation of cDNAs of scrapie-modulated RNAs by subtractive hybridization of a cDNA library. Proc Natl Acad Sci U S A 85:5738–5742 [CrossRef]
     [Google Scholar]
  19. Duguid J. R., Bohmont C. W., Liu N. G., Tourtellotte W. W. 1989; Changes in brain gene expression shared by scrapie and Alzheimer disease. Proc Natl Acad Sci U S A 86:7260–7264 [CrossRef]
     [Google Scholar]
  20. Etienne W., Meyer M. H., Peppers J., Meyer R. A., Jr. 2004; Comparison of mRNA gene expression by RT-PCR and DNA microarray. Biotechniques 36:618–620
     [Google Scholar]
  21. Finley D., Ciechanover A., Varshavsky A. 2004; Ubiquitin as a central cellular regulator 22 p following S32. Cell 116:S29–S32 [CrossRef]
     [Google Scholar]
  22. Giese A., Groschup M. H., Hess B., Kretzschmar H. A. 1995; Neuronal cell death in scrapie-infected mice is due to apoptosis. Brain Pathol 5:213–221 [CrossRef]
     [Google Scholar]
  23. Greenwood A. D., Horsch M., Stengel A., Vorberg I., Lutzny G., Maas E., Schadler S., Erfle V., Beckers J. other authors 2005; Cell line dependent RNA expression profiles of prion-infected mouse neuronal cells. J Mol Biol 349:487–500 [CrossRef]
     [Google Scholar]
  24. Hanlon J., Monks E., Hughes C., Weavers E., Rogers M. 2002; Metallothionein in bovine spongiform encephalopathy. J Comp Pathol 127:280–289 [CrossRef]
     [Google Scholar]
  25. Jin T., Gu Y., Zanusso G., Sy M., Kumar A., Cohen M., Gambetti P., Singh N. 2000; The chaperone protein BiP binds to a mutant prion protein and mediates its degradation by the proteasome. J Biol Chem 275:38699–38704 [CrossRef]
     [Google Scholar]
  26. Kenward N., Hope J., Landon M., Mayer R. J. 1994; Expression of polyubiquitin and heat-shock protein 70 genes increases in the later stages of disease progression in scrapie-infected mouse brain. J Neurochem 62:1870–1877
     [Google Scholar]
  27. Kim B. H., Lee H. G., Choi J. K., Kim J. I., Choi E. K., Carp R. I., Kim Y. S. 2004; The cellular prion protein (PrPC) prevents apoptotic neuronal cell death and mitochondrial dysfunction induced by serum deprivation. Brain Res Mol Brain Res 124:40–50 [CrossRef]
     [Google Scholar]
  28. Kordek R., Liberski P. P., Yanagihara R., Isaacson S., Gajdusek D. C. 1997; Molecular analysis of prion protein (PrP) and glial fibrillary acidic protein (GFAP) transcripts in experimental Creutzfeldt–Jakob disease in mice. Acta Neurobiol Exp (Wars) 57:85–90
     [Google Scholar]
  29. Laszlo L., Lowe J., Self T., Kenward N., Landon M., McBride T., Farquhar C., McConnell I., Brown J. other authors 1992; Lysosomes as key organelles in the pathogenesis of prion encephalopathies. J Pathol 166:333–341 [CrossRef]
     [Google Scholar]
  30. Lazarini F., Boussin F., Deslys J. P., Tardy M., Dormont D. 1994; Astrocyte gene expression in experimental mouse scrapie. J Comp Pathol 111:87–98 [CrossRef]
     [Google Scholar]
  31. Livak K. J., Schmittgen T. D. 2001; Analysis of relative gene expression data using real-time quantitative PCR and the method. Methods 25:402–408 [CrossRef]
     [Google Scholar]
  32. Lowe J., Fergusson J., Kenward N., Laszlo L., Landon M., Farquhar C., Brown J., Hope J., Mayer R. J. 1992; Immunoreactivity to ubiquitin-protein conjugates is present early in the disease process in the brains of scrapie-infected mice. J Pathol 168:169–177 [CrossRef]
     [Google Scholar]
  33. Manuelidis L., Tesin D. M., Sklaviadis T., Manuelidis E. E. 1987; Astrocyte gene expression in Creutzfeldt–Jakob disease. Proc Natl Acad Sci U S A 84:5937–5941 [CrossRef]
     [Google Scholar]
  34. Rao R. V., Hermel E., Castro-Obregon S., del Rio G., Ellerby L. M., Ellerby H. M., Bredesen D. E. 2001; Coupling endoplasmic reticulum stress to the cell death program. Mechanism of caspase activation. J Biol Chem 276:33869–33874 [CrossRef]
     [Google Scholar]
  35. Riemer C., Queck I., Simon D., Kurth R., Baier M. 2000; Identification of upregulated genes in scrapie-infected brain tissue. J Virol 74:10245–10248 [CrossRef]
     [Google Scholar]
  36. Riemer C., Neidhold S., Burwinkel M., Schwarz A., Schultz J., Kratzschmar J., Monning U., Baier M. 2004; Gene expression profiling of scrapie-infected brain tissue. Biochem Biophys Res Commun 323:556–564 [CrossRef]
     [Google Scholar]
  37. Schroeder A., Mueller O., Stocker S., Salowsky R., Leiber M., Gassmann M., Lightfoot S., Menzel W., Granzow M., Ragg T. 2006; The RIN: an RNA integrity number for assigning integrity values to RNA measurements. BMC Mol Biol 7:3 [CrossRef]
     [Google Scholar]
  38. Tanaka T. S., Jaradat S. A., Lim M. K., Kargul G. J., Wang X., Grahovac M. J., Pantano S., Sano Y., Piao Y. other authors 2000; Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray. Proc Natl Acad Sci U S A 97:9127–9132 [CrossRef]
     [Google Scholar]
  39. Tatzelt J., Zuo J., Voellmy R., Scott M., Hartl U., Prusiner S. B., Welch W. J. 1995; Scrapie prions selectively modify the stress response in neuroblastoma cells. Proc Natl Acad Sci U S A 92:2944–2948 [CrossRef]
     [Google Scholar]
  40. Tatzelt J., Voellmy R., Welch W. J. 1998; Abnormalities in stress proteins in prion diseases. Cell Mol Neurobiol 18:721–729 [CrossRef]
     [Google Scholar]
  41. Valko M., Morris H., Cronin M. T. 2005; Metals, toxicity and oxidative stress. Curr Med Chem 12:1161–1208 [CrossRef]
     [Google Scholar]
  42. Vandesompele J., De Preter K., Pattyn F., Poppe B., Van Roy N., De Paepe A., Speleman F. 2002; Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol 3 research0034:
     [Google Scholar]
  43. Wittwer C. T., Herrmann M. G., Moss A. A., Rasmussen R. P. 1997; Continuous fluorescence monitoring of rapid cycle DNA amplification. Biotechniques 22:130–131
     [Google Scholar]
  44. Xiang W., Windl O., Wunsch G., Dugas M., Kohlmann A., Dierkes N., Westner I. M., Kretzschmar H. A. 2004; Identification of differentially expressed genes in scrapie-infected mouse brains by using global gene expression technology. J Virol 78:11051–11060 [CrossRef]
     [Google Scholar]
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Molecular analysis of bovine spongiform encephalopathy infection by cDNA arrays
J. Gen. Virol. 88, 1356 (2007); https://doi.org/10.1099/vir.0.82387-0
/content/journal/jgv/10.1099/vir.0.82387-0
/content/journal/jgv/10.1099/vir.0.82387-0
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