@article{mbs:/content/journal/jgv/10.1099/vir.0.82493-0, author = "Hanke, Tomáš and McMichael, Andrew J. and Dorrell, Lucy", title = "Clinical experience with plasmid DNA- and modified vaccinia virus Ankara-vectored human immunodeficiency virus type 1 clade A vaccine focusing on T-cell induction", journal= "Journal of General Virology", year = "2007", volume = "88", number = "1", pages = "1-12", doi = "https://doi.org/10.1099/vir.0.82493-0", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.82493-0", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "Candidate human immunodeficiency virus type 1 (HIV-1) vaccines focusing on T-cell induction, constructed as pTHr.HIVA DNA and modified vaccinia virus Ankara (MVA).HIVA, were delivered in a heterologous prime–boost regimen. The vaccines were tested in several hundred healthy or HIV-1-infected volunteers in Europe and Africa. Whilst larger trials of hundreds of volunteers suggested induction of HIV-1-specific T-cell responses in <15 % of healthy vaccinees, a series of small, rapid trials in 12–24 volunteers at a time with a more in-depth analysis of vaccine-elicited T-cell responses proved to be highly informative and provided more encouraging results. These trials demonstrated that the pTHr.HIVA vaccine alone primed consistently weak and mainly CD4+, but also CD8+ T-cell responses, and the MVA.HIVA vaccine delivered a consistent boost to both CD4+ and CD8+ T cells, which was particularly strong in HIV-1-infected patients. Thus, whilst the search is on for ways to enhance T-cell priming, MVA is a useful boosting vector for human subunit genetic vaccines.", }