1887

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Volume 88, Issue 5

Complete genomes for hepatitis C virus subtypes 6f, 6i, 6j and 6m: viral genetic diversity among Thai blood donors and infected spouses Free

Abstract

In this study, the first complete genome sequences for hepatitis C virus (HCV) subtypes 6f, 6i, 6j and 6m, obtained from infected blood donors in Chiang Mai, Thailand, are reported. Pairwise genome-wide nucleotide similarities between some of these isolates were higher than the 75–80 % value used previously to define different HCV subtypes. To investigate further, the entire genomes of four prototype isolates, Th602 (6i), Th553 (6j), B4/92 (6m) and D86/93 (6n), were sequenced. Pairwise comparison of these sequences gave a similar range of nucleotide similarities, thereby providing new information for HCV subtype classification. In order to study the hypothesis of interspousal HCV transmission, four additional complete HCV genome sequences were obtained from two infected Thai blood donors and their spouses, C-0044 and C-0046 (6f), and C-0192 and C-0185 (6m). Pairwise comparison of the sequences revealed that C-0044 and C-0046 share a nucleotide similarity of 98.1 %, whilst C-0185 and C-0192 have a similarity of 97.8 %. Several other studies of partial HCV sequences of different genomic regions from HCV-infected couples have shown nucleotide similarities ranging from 96.3 to 100 %. The similarities of the complete genome sequences from the two couples in the current study are consistent with HCV transmission between spouses.

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2007-05-01
2024-04-19
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References

  1. Akahane Y., Kojima M., Sugai Y., Sakamoto M., Miyazaki Y., Tanaka T., Tsuda F., Mishiro S., Okamoto H. other authors 1994; Hepatitis C virus infection in spouses of patients with type C chronic liver disease. Ann Intern Med 120:748–752 [CrossRef]
     [Google Scholar]
  2. Alter M. J. 1994; Transmission of hepatitis C virus – route, dose, and titer. N Engl J Med 330:784–786 [CrossRef]
     [Google Scholar]
  3. Alter M. J., Hadler S. C., Judson F. N., Mares A., Alexander W. J., Hu P. Y., Miller J. K., Moyer L. A., Fields H. A. other authors 1990; Risk factors for acute non-A, non-B hepatitis in the United States and association with hepatitis C virus infection. JAMA 264:2231–2235 [CrossRef]
     [Google Scholar]
  4. Apichartpiyakul C., Chittivudikarn C., Miyajima H., Homma M., Hotta H. 1994; Analysis of hepatitis C virus isolates among healthy blood donors and drug addicts in Chiang Mai, Thailand. J Clin Microbiol 32:2276–2279
     [Google Scholar]
  5. Beyrer C., Sripaipan T., Tovanabutra S., Jittiwutikarn J., Suriyanon V., Vongchak T., Srirak N., Kawichai S., Razak M. H., Celentano D. D. 2005; High HIV, hepatitis C and sexual risks among drug-using men who have sex with men in northern Thailand. AIDS 19:1535–1540 [CrossRef]
     [Google Scholar]
  6. Capelli C., Prati D., Bosoni P., Zanuso F., Pappalettera M., Mozzi F., De Mattei C., Zanella A., Sirchia G. 1997; Sexual transmission of hepatitis C virus to a repeat blood donor. Transfusion 37:436–440 [CrossRef]
     [Google Scholar]
  7. Chayama K., Kobayashi M., Tsubota A., Koida I., Arase Y., Saitoh S., Ikeda K., Kumada H. 1995; Molecular analysis of intraspousal transmission of hepatitis C virus. J Hepatol 22:431–439 [CrossRef]
     [Google Scholar]
  8. Daikos G. L., Lai S., Fischl M. A. 1994; Hepatitis C virus infection in a sexually active inner city population. The potential for heterosexual transmission. Infection 22:72–76 [CrossRef]
     [Google Scholar]
  9. Doi H., Apichartpiyakul C., Ohba K. I., Mizokami M., Hotta H. 1996; Hepatitis C virus (HCV) subtype prevalence in Chiang Mai, Thailand, and identification of novel subtypes of HCV major type 6. J Clin Microbiol 34:569–574
     [Google Scholar]
  10. Guindon S., Gascuel O. 2003; A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Syst Biol 52:696–704 [CrossRef]
     [Google Scholar]
  11. Halfon P., Riflet H., Renou C., Quentin Y., Cacoub P. 2001; Molecular evidence of male-to-female sexual transmission of hepatitis C virus after vaginal and anal intercourse. J Clin Microbiol 39:1204–1206 [CrossRef]
     [Google Scholar]
  12. Hasegawa M., Kishino H., Yano T. 1985; Dating of the human–ape splitting by a molecular clock of mitochondrial DNA. J Mol Evol 22:160–174 [CrossRef]
     [Google Scholar]
  13. Healey C. J., Smith D. B., Walker J. L., Holmes E. C., Fleming K. A., Chapman R. W., Simmonds P. 1995; Acute hepatitis C infection after sexual exposure. Gut 36:148–150 [CrossRef]
     [Google Scholar]
  14. Honda M., Kaneko S., Unoura M., Kobayashi K., Murakami S. 1993; Risk of hepatitis C virus infections through household contact with chronic carriers: analysis of nucleotide sequences. Hepatology 17:971–976 [CrossRef]
     [Google Scholar]
  15. Kao J. H., Chen P. J., Yang P. M., Lai M. Y., Sheu J. C., Wang T. H., Chen D. S. 1992; Intrafamilial transmission of hepatitis C virus: the important role of infections between spouses. J Infect Dis 166:900–903 [CrossRef]
     [Google Scholar]
  16. Kao J. H., Liu C. J., Chen P. J., Chen W., Lai M. Y., Chen D. S. 2000; Low incidence of hepatitis C virus transmission between spouses: a prospective study. J Gastroenterol Hepatol 15:391–395 [CrossRef]
     [Google Scholar]
  17. Komine F., Yamaguchi T., Moriyama M., Segawa T., Matsumura H., Nakai K., Shimizu T., Hayashi N., Arakawa Y. other authors 1999; A case of sexually transmitted acute hepatitis C: confirmation by analysis of viral genome. Hepatol Res 14:84–91 [CrossRef]
     [Google Scholar]
  18. Kuiken C., Yusim K., Boykin L., Richardson R. 2005; The Los Alamos hepatitis C sequence database. Bioinformatics 21:379–384 [CrossRef]
     [Google Scholar]
  19. Kuiken C., Combet C., Bukh J., Shin-I T., Deleage G., Mizokami M., Richardson R., Sablon E., Yusim K. other authors 2006; A comprehensive system for consistent numbering of HCV sequences, proteins and epitopes. Hepatology 44:1355–1361 [CrossRef]
     [Google Scholar]
  20. Kumar S., Tamura K., Nei M. 2004; mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163 [CrossRef]
     [Google Scholar]
  21. Li C., Fu Y., Lu L., Ji W., Yu J., Hagedorn C. H., Zhang L. 2006; Complete genomic sequences for hepatitis C virus subtypes 6e and 6g isolated from Chinese patients with injection drug use and HIV-1 co-infection. J Med Virol 78:1061–1069 [CrossRef]
     [Google Scholar]
  22. Lu L., Nakano T., Li C., Fu Y., Miller S., Kuiken C., Robertson B. H., Hagedorn C. H. 2006; Hepatitis C virus complete genome sequences identified from China representing subtypes 6k and 6n and a novel, as yet unassigned subtype within genotype 6. J Gen Virol 87:629–634 [CrossRef]
     [Google Scholar]
  23. Luksamijarulkul P., Deangbubpha A. 1997; Hepatitis C antibody prevalence and risk factors of some female sex workers in Thailand. Southeast Asian J Trop Med Public Health 28:507–512
     [Google Scholar]
  24. Luksamijarulkul P., Plucktaweesak S. 1996; High hepatitis C seroprevalence in Thai intravenous drug abusers and qualitative risk analysis. Southeast Asian J Trop Med Public Health 27:654–658
     [Google Scholar]
  25. Luksamijarulkul P., Khemnak P., Pacheun O. 2000; Human immunodeficiency virus and hepatitis C virus infections among patients attending sexually transmitted disease clinics, Regional 2, Thailand. Asia Pac J Public Health 12:41–45 [CrossRef]
     [Google Scholar]
  26. Luksamijarulkul P., Thammata N., Sujirarat D., Tiloklurs M. 2004; Hepatitis C virus infection among Thai blood donors: antibody prevalence, risk factors and development of risk screening form. Southeast Asian J Trop Med Public Health 35:147–154
     [Google Scholar]
  27. Martin D. P., Williamson C., Posada D. 2005; rdp2: recombination detection and analysis from sequence alignments. Bioinformatics 21:260–262 [CrossRef]
     [Google Scholar]
  28. Mellor J., Holmes E. C., Jarvis L. M., Yap P. L., Simmonds P. 1995; Investigation of the pattern of hepatitis C virus sequence diversity in different geographical regions: implications for virus classification. The International HCV Collaborative Study Group. J Gen Virol 76:2493–2507 [CrossRef]
     [Google Scholar]
  29. Mellor J., Walsh E. A., Prescott L. E., Jarvis L. M., Davidson F., Yap P. L., Simmonds P. 1996; Survey of type 6 group variants of hepatitis C virus in Southeast Asia by using a core-based genotyping assay. J Clin Microbiol 34:417–423
     [Google Scholar]
  30. Mesquita P. E., Granato C. F., Castelo A. 1997; Risk factors associated with hepatitis C virus (HCV) infection among prostitutes and their clients in the city of Santos, Sao Paulo State, Brazil. J Med Virol 51:338–343 [CrossRef]
     [Google Scholar]
  31. Moreno R., Berenguer M. 2002; Hepatitis C and liver transplantation. Ann Hepatol 1:129–135
     [Google Scholar]
  32. Morrison L. 2004; Traditions in transition: young people's risk for HIV in Chiang Mai, Thailand. Qual Health Res 14:328–344 [CrossRef]
     [Google Scholar]
  33. Murphy E. L., Bryzman S. M., Glynn S. A., Ameti D. I., Thomson R. A., Williams A. E., Nass C. C., Ownby H. E., Schreiber G. B. other authors 2000; Risk factors for hepatitis C virus infection in United States blood donors. NHLBI Retrovirus Epidemiology Donor Study (REDS). Hepatology 31:756–762 [CrossRef]
     [Google Scholar]
  34. Nakayama H., Sugai Y., Ikeya S., Inoue J., Nishizawa T., Okamoto H. 2005; Molecular investigation of interspousal transmission of hepatitis C virus in two Japanese patients who acquired acute hepatitis C after 40 or 42 years of marriage. J Med Virol 75:258–266 [CrossRef]
     [Google Scholar]
  35. Nantachit N., Robison V., Wongthanee A., Kamtorn N., Suriyanon V., Nelson K. E. 2003; Temporal trends in the prevalence of HIV and other transfusion-transmissible infections among blood donors in northern Thailand, 1990 through 2001. Transfusion 43:730–735 [CrossRef]
     [Google Scholar]
  36. Ndjomou J., Pybus O. G., Matz B. 2003; Phylogenetic analysis of hepatitis C virus isolates indicates a unique pattern of endemic infection in Cameroon. J Gen Virol 84:2333–2341 [CrossRef]
     [Google Scholar]
  37. Ogata N., Alter H. J., Miller R. H., Purcell R. H. 1991; Nucleotide sequence and mutation rate of the H strain of hepatitis C virus. Proc Natl Acad Sci U S A 88:3392–3396 [CrossRef]
     [Google Scholar]
  38. Osmond D. H., Charlebois E., Sheppard H. W., Page K., Winkelstein W., Moss A. R., Reingold A. 1993; Comparison of risk factors for hepatitis C and hepatitis B virus infection in homosexual men. J Infect Dis 167:66–71 [CrossRef]
     [Google Scholar]
  39. Pybus O. G., Charleston M. A., Gupta S., Rambaut A., Holmes E. C., Harvey P. H. 2001; The epidemic behavior of the hepatitis C virus. Science 292:2323–2325 [CrossRef]
     [Google Scholar]
  40. Quer J., Murillo P., Esteban J. I., Martell M., Esteban R., Guardia J. 2003; Sexual transmission of hepatitis C virus from a patient with chronic disease to his sex partner after removal of an intrauterine device. Sex Transm Dis 30:470–471 [CrossRef]
     [Google Scholar]
  41. Rice P. S., Smith D. B., Simmonds P., Holmes E. 1993; Heterosexual transmission of hepatitis C virus. Lancet 342:1052–1053
     [Google Scholar]
  42. Romanowski B., Preiksaitis J., Campbell P., Fenton J. 2003; Hepatitis C seroprevalence and risk behaviors in patients attending sexually transmitted disease clinics. Sex Transm Dis 30:33–38 [CrossRef]
     [Google Scholar]
  43. Ross R. S., Viazov S., Varenholz C., Roggendorf M. 1999; Inquiries on intraspousal transmission of hepatitis C virus: benefits and limitations of genome sequencing and phylogenetic analysis. Forensic Sci Int 100:69–76 [CrossRef]
     [Google Scholar]
  44. Shinji T., Kyaw Y. Y., Gokan K., Tanaka Y., Ochi K., Kusano N., Mizushima T., Fujioka S., Shiraha H. other authors 2004; Analysis of HCV genotypes from blood donors shows three new HCV type 6 subgroups exist in Myanmar. Acta Med Okayama 58:135–142
     [Google Scholar]
  45. Simmonds P. 2004; Genetic diversity and evolution of hepatitis C virus – 15 years on. J Gen Virol 85:3173–3188 [CrossRef]
     [Google Scholar]
  46. Simmonds P., Alberti A., Alter H. J., Bonino F., Bradley D. W., Brechot C., Brouwer J. T., Chan S. W., Chayama K., Chen D. S. 1994; A proposed system for the nomenclature of hepatitis C viral genotypes. Hepatology 19:1321–1324 [CrossRef]
     [Google Scholar]
  47. Simmonds P., Mellor J., Sakuldamrongpanich T., Nuchaprayoon C., Tanprasert S., Holmes E. C., Smith D. B. 1996; Evolutionary analysis of variants of hepatitis C virus found in South-East Asia: comparison with classifications based upon sequence similarity. J Gen Virol 77:3013–3014 [CrossRef]
     [Google Scholar]
  48. Simmonds P., Bukh J., Combet C., Deleage G., Enomoto N., Feinstone S., Halfon P., Inchauspe G., Kuiken C. other authors 2005; Consensus proposals for a unified system of nomenclature of hepatitis C virus genotypes. Hepatology 42:962–973 [CrossRef]
     [Google Scholar]
  49. Songsivilai S., Jinathongthai S., Wongsena W., Tiangpitayakorn C., Dharakul T. 1997; High prevalence of hepatitis C infection among blood donors in northeastern Thailand. Am J Trop Med Hyg 57:66–69
     [Google Scholar]
  50. Stroffolini T., Lorenzoni U., Menniti-Ippolito F., Infantolino D., Chiaramonte M. 2001; Hepatitis C virus infection in spouses: sexual transmission or common exposure to the same risk factors?. Am J Gastroenterol 96:3138–3141 [CrossRef]
     [Google Scholar]
  51. Sugiyama K., Kato N., Nakazawa T., Yonemura Y., Phornphutkul K., Kunakorn M., Petchclai B., Shimotohno K. 1995; Novel genotypes of hepatitis C virus in Thailand. J Gen Virol 76:2323–2327 [CrossRef]
     [Google Scholar]
  52. Terrault N. A. 2002; Sexual activity as a risk factor for hepatitis C. Hepatology 36:S99–S105 [CrossRef]
     [Google Scholar]
  53. Thaikruea L., Thongsawat S., Maneekarn N., Netski D., Thomas D. L., Nelson K. E. 2004; Risk factors for hepatitis C virus infection among blood donors in northern Thailand. Transfusion 44:1433–1440 [CrossRef]
     [Google Scholar]
  54. Theamboonlers A., Chinchai T., Bedi K., Jantarasamee P., Sripontong M., Poovorawan Y. 2002; Molecular characterization of hepatitis C virus (HCV) core region in HCV-infected Thai blood donors. Acta Virol 46:169–173
     [Google Scholar]
  55. Thomas D. L., Zenilman J. M., Alter H. J., Shih J. W., Galai N., Carella A. V., Quinn T. C. 1995; Sexual transmission of hepatitis C virus among patients attending sexually transmitted diseases clinics in Baltimore – an analysis of 309 sex partnerships. J Infect Dis 171:768–775 [CrossRef]
     [Google Scholar]
  56. Tokita H., Okamoto H., Luengrojanakul P., Vareesangthip K., Chainuvati T., Iizuka H., Tsuda F., Miyakawa Y., Mayumi M. 1995; Hepatitis C virus variants from Thailand classifiable into five novel genotypes in the sixth (6b), seventh (7c, 7d) and ninth (9b, 9c) major genetic groups. . J Gen Virol 76:2329–2335 [CrossRef]
     [Google Scholar]
  57. Weniger B. G., Limpakarnjanarat K., Ungchusak K., Thanprasertsuk S., Choopanya K., Vanichseni S., Uneklabh T., Thongcharoen P., Wasi C. 1991; The epidemiology of HIV infection and AIDS in Thailand. AIDS 5, (Suppl. 2):S71–S85 [CrossRef]
     [Google Scholar]
  58. Wiwanitkit V. 2005; Anti HCV seroprevalence among the voluntary blood donors in Thailand. Hematology 10:431–433 [CrossRef]
     [Google Scholar]
  59. Wiwanitkit V., Suyaphan A. 2002; A note in the high prevalence of anti-HCV seropositivity among hilltribers in Mae Jam District, Northern Thailand. Viral Immunol 15:645–646 [CrossRef]
     [Google Scholar]
  60. Yagura M., Fukui H., Murai S., Tokita H., Kamitsukasa H., Harada H., Sumida H. 2002; Intraspousal transmission of hepatitis C virus occurring after 42 years of marriage: no relation to HCV RNA titers. Am J Gastroenterol 97:2925–2927
     [Google Scholar]
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Complete genomes for hepatitis C virus subtypes 6f, 6i, 6j and 6m: viral genetic diversity among Thai blood donors and infected spouses
J. Gen. Virol. 88, 1505 (2007); https://doi.org/10.1099/vir.0.82604-0
/content/journal/jgv/10.1099/vir.0.82604-0
/content/journal/jgv/10.1099/vir.0.82604-0
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