RT Journal Article SR Electronic(1) A1 Fryer, Jacqueline F. A1 Delwart, Eric A1 Bernardin, Flavien A1 Tuke, Philip W. A1 Lukashov, Vladimir V. A1 Baylis, Sally A.YR 2007 T1 Analysis of two human parvovirus PARV4 genotypes identified in human plasma for fractionation JF Journal of General Virology, VO 88 IS 8 SP 2162 OP 2167 DO https://doi.org/10.1099/vir.0.82620-0 PB Microbiology Society, SN 1465-2099, AB The presence of the novel parvovirus PARV4 and a related variant, PARV5, was recently demonstrated in pooled plasma used in the manufacture of blood and plasma-derived medicinal products. DNA sequence analysis of nearly full-length genomes of four PARV4 and two PARV5 strains from manufacturing plasma pools is now presented. Like PARV4, PARV5 encodes two non-overlapping open reading frames (ORF1 and ORF2), homologous to the non-structural and capsid proteins of other parvoviruses, respectively. A highly conserved region in ORF2 contains phospholipase A2 motifs involved in parvovirus infectivity. Hybridization of strand-specific probes to DNA extracted from high-titre, PARV4-positive plasma revealed that the positive and negative strands are packaged into PARV4 virions in similar quantities. This extended analysis of nearly full-length PARV4 and PARV5 sequences suggests that they are closely related genotypes and the use of a single virus name, PARV4, comprising genotypes 1 and 2 (previously termed PARV5) is proposed., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.82620-0