1887

Abstract

The genus is composed of segmented, negative-sense RNA viruses that are responsible for mild to severe human diseases. To date, no molecular studies of bunyaviruses in the genus from central Africa have been reported, and their classification relies on serological testing. Four new primer pairs for RT-PCR amplification and sequencing of the complete genomic small (S) RNA segments of 10 orthobunyaviruses isolated from the Central African Republic and pertaining to five different serogroups have been designed and evaluated. Phylogenetic analysis showed that these 10 viruses belong to the Bunyamwera serogroup. The S segment sequences differ from those of the Bunyamwera virus reference strain by 5–15 % at the nucleotide level, and both overlapping reading frames, encoding the nucleocapsid (N) and non-structural (NS) proteins, were evident in sequenced genomes. This study should improve diagnosis and surveillance of African bunyaviruses.

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2007-06-01
2024-04-19
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References

  1. Artsob H., Spence L. P., Thing C. 1984; Enzyme-linked immunosorbent assay typing of California serogroup viruses isolated in Canada. J Clin Microbiol 20:276–280
    [Google Scholar]
  2. Bowen M. D., Jackson A. O., Bruns T. D., Hacker D. L., Hardy J. L. 1995; Determination and comparative analysis of the small RNA genomic sequences of California encephalitis, Jamestown Canyon, Jerry Slough, Melao, Keystone and Trivittatus viruses ( Bunyaviridae , genus Bunyavirus , California serogroup). J Gen Virol 76:559–572 [CrossRef]
    [Google Scholar]
  3. Bowen M. D., Trappier S. G., Sanchez A. J., Meyer R. F., Goldsmith C. S., Zaki S. R., Dunster L. M., Peters C. J., Ksiazek T. G., Nichol S. T. 2001; A reassortant bunyavirus isolated from acute hemorrhagic fever cases in Kenya and Somalia. . RVF Task Force. Virology 291:185–190 [CrossRef]
    [Google Scholar]
  4. Calisher C. H., Karabatsos N. 1988; Arbovirus serogroups: definition and geographic distribution. In The Arboviruses Epidemiology and Ecology pp 19–57 Edited by Monath T. P. Boca Raton, FL: CRC Press;
    [Google Scholar]
  5. Dunn E. F., Pritlove D. C., Elliott R. M. 1994; The S RNA genome segments of Batai, Cache Valley, Guaroa, Kairi, Lumbo, Main Drain and Northway bunyaviruses: sequence determination and analysis. J Gen Virol 75:597–608 [CrossRef]
    [Google Scholar]
  6. Elliott R. M. 1989; Nucleotide sequence analysis of the S RNA segment of Bunyamwera virus, the prototype of the family Bunyaviridae . J Gen Virol 70:1281–1285 [CrossRef]
    [Google Scholar]
  7. Elliott R. M. 1990; Molecular biology of Bunyaviridae . J Gen Virol 71:501–522 [CrossRef]
    [Google Scholar]
  8. Guindon S., Gascuel O. 2003; A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Syst Biol 52:696–704 [CrossRef]
    [Google Scholar]
  9. Hildreth S. W., Beaty B. J., Meegan J. M., Frazier C. L., Shope R. E. 1982; Detection of La Crosse arbovirus antigen in mosquito pools: application of chromogenic and fluorogenic enzyme immunoassay systems. J Clin Microbiol 15:879–884
    [Google Scholar]
  10. Huang C., Shope R. E., Spargo B., Campbell W. P. 1996; The S RNA genomic sequences of Inkoo, San Angelo, Serra do Navio, South River, and Tahyna bunyaviruses. J Gen Virol 77:1761–1768 [CrossRef]
    [Google Scholar]
  11. Shope R. E., Causey O. R. 1962; Further studies on the serological relationships of group C arthropod-borne viruses and the application of these relationships to rapid identification of types. Am J Trop Med Hyg 11:283
    [Google Scholar]
  12. Smithburn K. C., Haddow A. J., Mahaffy A. F. 1946; A neurotropic virus isolated from Aedes mosquitoes caught in the Semliki Forest. Am J Trop Med 26:189–208
    [Google Scholar]
  13. Soldan S. S., González-Scarano F. 2005; Emerging infectious diseases: the Bunyaviridae . J Neurovirol 11:412–423 [CrossRef]
    [Google Scholar]
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