@article{mbs:/content/journal/jgv/10.1099/vir.0.82990-0, author = "Peri, Piritta and Hukkanen, Veijo and Nuutila, Kristiina and Saukko, Pekka and Abrahamson, Magnus and Vuorinen, Tytti", title = "The cysteine protease inhibitors cystatins inhibit herpes simplex virus type 1-induced apoptosis and virus yield in HEp-2 cells", journal= "Journal of General Virology", year = "2007", volume = "88", number = "8", pages = "2101-2105", doi = "https://doi.org/10.1099/vir.0.82990-0", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.82990-0", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "The role of cystatins in herpes simplex virus (HSV)-induced apoptosis and viral replication has been studied. Human epithelial (HEp-2) cells infected with wild-type HSV-1 (F), with a deletion virus lacking the anti-apoptotic gene Us3 (R7041) or with a deletion virus lacking the anti-apoptotic genes Us3 and ICP4 (d120) were treated with cystatin A, C or D. Cells and culture media were studied at different time points for replicating HSV-1 and for apoptosis. Cystatins C and D inhibited the yield of replicative HSV-1 significantly in HEp-2 cells. In addition, cystatin D inhibited R7041 and d120 virus-induced apoptosis. Moreover, cystatin A inhibited R7041-induced apoptosis. These inhibitory effects of cystatins on virus replication and apoptosis are likely to be separate functions. Cystatin D treatment decreased cellular cathepsin B activity in HSV-1 infection, suggesting that cathepsin B is involved in virus-induced apoptosis.", }