1887

Abstract

Viral infections usually take place in an orderly manner and can be divided into at least two phases: an early and a late stage. In geminiviruses, plant viruses with a circular, single-stranded DNA genome, expression of viral genes involves complex regulation strategies that suggest the existence of a pattern of temporal gene expression. In this work, the transcription of pepper huasteco yellow vein virus (PHYVV) genes was studied. Green fluorescent protein replacements and RT-PCR analyses were used to monitor PHYVV gene expression chronologically in suspension cells and plant tissue. A model is proposed to describe the order of geminivirus gene expression, where the genes that encode Rep, TrAP and REn are expressed during an early stage of infection. The genes that encode the coat protein and the nuclear shuttle protein are expressed during the late stage of infection.

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2007-11-01
2024-04-16
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References

  1. Alberter B., Rezaian M. A., Jeske H. 2005; Replicative intermediates of Tomato leaf curl virus and its satellite DNAs. Virology 331:441–448 [CrossRef]
    [Google Scholar]
  2. Argüello-Astorga G. R., Guevara-González R. G., Herrera-Estrella L. R., Rivera-Bustamante R. F. 1994; Geminivirus replication origins have a group-specific organization of iterative elements: a model for replication. Virology 203:90–100 [CrossRef]
    [Google Scholar]
  3. Balakrishnan L., Milavetz B. 2006; Reorganization of RNA polymerase II on the SV40 genome occurs coordinately with the early to late transcriptional switch. Virology 345:31–43 [CrossRef]
    [Google Scholar]
  4. Bass H. W., Nagar S., Hanley-Bowdoin L., Robertson D. 2000; Chromosome condensation induced by geminivirus infection of mature plant cells. J Cell Sci 113:1149–1160
    [Google Scholar]
  5. Berk A. J. 1986; Adenovirus promoters and E1a transactivation. Annu Rev Genet 20:45–79 [CrossRef]
    [Google Scholar]
  6. Bisaro D. M. 2006; Silencing suppression by geminivirus proteins. Virology 344:158–168 [CrossRef]
    [Google Scholar]
  7. Brough C. L., Sunter V., Gardiner W. E., Bisaro D. M. 1992; Kinetics of Tomato golden mosaic virus DNA replication and coat protein promoter activity in Nicotiana tabacum protoplasts. Virology 187:1–9 [CrossRef]
    [Google Scholar]
  8. Cabrera-Ponce J. L., López L., Assad-Garcia N., Medina-Arevalo C., Bailey A. M., Herrera-Estrella L. 1997; An efficient particle bombardment system for the genetic transformation of asparagus ( Asparagus officinalis L.). Plant Cell Rep 16:255–260
    [Google Scholar]
  9. Camacho-Villasana Y. M., Ochoa-Alejo N., Walling L., Bray E. A. 2002; An improved method for isolating RNA from dehydrated and nondehydrated chili pepper ( Capsicum annuum L.) plant tissues. Plant Mol Biol Rep 20:407–414 [CrossRef]
    [Google Scholar]
  10. Cook W. J., Coen D. M. 1996; Temporal regulation of herpes simplex virus type 1 UL24 mRNA expression via differential polyadenylation. Virology 218:204–213 [CrossRef]
    [Google Scholar]
  11. Eagle P. A., Hanley-Bowdoin L. 1997; cis elements that contribute to geminivirus transcriptional regulation and the efficiency of DNA replication. J Virol 71:6947–6955
    [Google Scholar]
  12. Eagle P. A., Orozco B. M., Hanley-Bowdoin L. 1994; A DNA sequence required for geminivirus replication also mediates transcriptional regulation. Plant Cell 6:1157–1170 [CrossRef]
    [Google Scholar]
  13. Fanning E., Knippers R. 1992; Structure and function of simian virus 40 large tumor antigen. Annu Rev Biochem 61:55–85 [CrossRef]
    [Google Scholar]
  14. Farrell M. L., Mertz J. E. 2002; Hormone response element in SV40 late promoter directly affects synthesis of early as well as late viral RNAs. Virology 297:307–318 [CrossRef]
    [Google Scholar]
  15. Frischmuth S., Frischmuth T., Jeske H. 1991; Transcript mapping of Abutilon mosaic virus , a geminivirus. Virology 185:596–604 [CrossRef]
    [Google Scholar]
  16. Garzón-Tiznado J. A., Torres-Pacheco I., Ascencio-Ibañez J. T., Herrera-Estrella L., Rivera-Bustamante R. F. 1993; Inoculation of peppers with infectious clones of a new geminivirus by a biolistic procedure. Phytopathology 83:514–521 [CrossRef]
    [Google Scholar]
  17. Haley A., Zhan X., Richardson K., Head K., Morris B. 1992; Regulation of the activities of African cassava mosaic virus promoters by the AC1, AC2, and AC3 gene products. Virology 188:905–909 [CrossRef]
    [Google Scholar]
  18. Hanley-Bowdoin L., Elmer J. S., Rogers S. G. 1989; Functional expression of the leftward open reading frames of the A component of Tomato golden mosaic virus in transgenic tobacco plants. Plant Cell 1:1057–1067 [CrossRef]
    [Google Scholar]
  19. Hanley-Bowdoin L., Elmer J. S., Rogers S. G. 1990; Expression of functional replication protein from Tomato golden mosaic virus in transgenic tobacco plants. Proc Natl Acad Sci U S A 87:1446–1450 [CrossRef]
    [Google Scholar]
  20. Hanley-Bowdoin L., Settlage S. B., Orozco B. M., Nagar S., Robertson D. 2000; Geminiviruses: models for plant DNA replication, transcription, and cell cycle regulation. Crit Rev Biochem Mol Biol 35:105–140
    [Google Scholar]
  21. Hartzell S. W., Byrne B. J., Subramanian K. N. 1984; Mapping of the late promoter of simian virus 40. Proc Natl Acad Sci U S A 81:23–27 [CrossRef]
    [Google Scholar]
  22. Hehnle S., Wege C., Jeske H. 2004; Interaction of DNA with the movement proteins of geminiviruses revisited. J Virol 78:7698–7706 [CrossRef]
    [Google Scholar]
  23. Heim R., Cubitt A. B., Tsien R. Y. 1995; Improved green fluorescence. Nature 373:663–664
    [Google Scholar]
  24. Jeffrey J. L., Pooma W., Petty I. T. 1996; Genetic requirements for local and systemic movement of Tomato golden mosaic virus in infected plants. Virology 223:208–218 [CrossRef]
    [Google Scholar]
  25. Kelly J. J., Wildeman A. G. 1991; Role of the SV40 enhancer in the early to late shift in viral transcription. Nucleic Acids Res 19:6799–6804 [CrossRef]
    [Google Scholar]
  26. Khoury G., May E. 1977; Regulation of early and late simian virus 40 transcription: overproduction of early viral RNA in the absence of a functional T-antigen. J Virol 23:167–176
    [Google Scholar]
  27. Méndez-Lozano J., Torres-Pacheco I., Fauquet C. M., Rivera-Bustamante R. F. 2003; Interactions between geminiviruses in a naturally occurring mixture: Pepper huasteco virus and Pepper golden mosaic virus . Phytopathology 93:270–277 [CrossRef]
    [Google Scholar]
  28. Menegazzi P., Galvan M., Rotola A., Ravaioli T., Gonelli A., Cassai E., Di Luca D. 1999; Temporal mapping of transcripts in human herpesvirus-7. J Gen Virol 80:2705–2712
    [Google Scholar]
  29. Mullineaux P. M., Ridgen J. E., Dry I. B., Krake L. R., Rezaian M. A. 1993; Mapping of the polycistronic RNAs of tomato leaf curl geminivirus. Virology 193:414–423 [CrossRef]
    [Google Scholar]
  30. Nevins J. R. 1991; Transcriptional activation by viral regulatory proteins. Trends Biochem Sci 16:435–439 [CrossRef]
    [Google Scholar]
  31. Noueiry A. O., Lucas W. J., Gilbertson R. L. 1994; Two proteins of a plant DNA virus coordinate nuclear and plasmodesmal transport. Cell 76:925–932 [CrossRef]
    [Google Scholar]
  32. Polston J. E., Anderson P. K. 1997; The emergence of whitefly-transmitted geminiviruses in tomato in the Western hemisphere. Plant Dis 81:1358–1369 [CrossRef]
    [Google Scholar]
  33. Preiss W., Jeske H. 2003; Multitasking in replication is common among geminiviruses. J Virol 77:2972–2980 [CrossRef]
    [Google Scholar]
  34. Reichel C., Mathur J., Eckes P., Langenkemper K., Koncz C., Schell J., Reiss B., Maas C. 1996; Enhanced green fluorescence by the expression of an Aequorea victoria green fluorescent protein mutant in mono- and dicotyledonous plant cells. Proc Natl Acad Sci U S A 93:5888–5893 [CrossRef]
    [Google Scholar]
  35. Rojas M. R., Hagen C., Lucas W. J., Gilbertson R. L. 2005; Exploiting chinks in the plant's armor: evolution and emergence of geminiviruses. Annu Rev Phytopathol 43:361–394 [CrossRef]
    [Google Scholar]
  36. Ruiz-Medrano R., Guevara-Gonzalez R. G., Arguello-Astorga G. R., Monsalve-Fonnegra Z., Herrera-Estrella L. R., Rivera-Bustamante R. F. 1999; Identification of a sequence element involved in AC2-mediated transactivation of the Pepper huasteco virus coat protein gene. Virology 253:162–169 [CrossRef]
    [Google Scholar]
  37. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual , 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press;
    [Google Scholar]
  38. Sanderfoot A. A., Lazarowitz S. G. 1996; Getting it together in plant virus movement: cooperative interactions between bipartite geminivirus movement proteins. Trends Cell Biol 6:353–358 [CrossRef]
    [Google Scholar]
  39. Sanderfoot A. A., Ingham D. J., Lazarowitz S. G. 1996; A viral movement protein as a nuclear shuttle. The geminivirus BR1 movement protein contains domains essential for interaction with BL1 and nuclear localization. Plant Physiol 110:23–33 [CrossRef]
    [Google Scholar]
  40. Saunders K., Lucy A., Stanley J. 1991; DNA forms of the geminivirus African cassava mosaic virus consistent with a rolling circle mechanism of replication. Nucleic Acids Res 19:2325–2330 [CrossRef]
    [Google Scholar]
  41. Shivaprasad P. V., Akbergenov R., Trinks D., Rajeswaran R., Veluthambi K., Hohn T., Poggin M. M. 2005; Promoters, transcripts, and regulatory proteins of mungbean yellow mosaic geminivirus. J Virol 79:8149–8163 [CrossRef]
    [Google Scholar]
  42. Shung C.-Y., Sunter G. 2007; AL1-dependent repression of transcription enhances expression of Tomato golden mosaic virus AL2 and AL3. Virology 364:112–122 [CrossRef]
    [Google Scholar]
  43. Shung C.-Y., Sunter J., Sirasanagandla S. S., Sunter G. 2006; Distinct viral sequence elements are necesary for expression of Tomato golden mosaic virus complementary sense transcripts that direct AL2 and AL3 gene expression. Mol Plant Microbe Interact 19:1394–1405 [CrossRef]
    [Google Scholar]
  44. Sunter G., Bisaro D. M. 1991; Transactivation in a geminivirus: AL2 gene product is needed for coat protein expression. Virology 180:416–419 [CrossRef]
    [Google Scholar]
  45. Sunter G., Bisaro D. M. 1992; Transactivation of geminivirus AR1 and BR1 gene expression by the viral AL2 gene product occurs at the level of transcription. Plant Cell 4:1321–1331 [CrossRef]
    [Google Scholar]
  46. Sunter G., Bisaro D. M. 1997; Regulation of a geminivirus coat protein promoter by AL2 protein (TrAP): evidence for activation and derepression mechanisms. Virology 232:269–280 [CrossRef]
    [Google Scholar]
  47. Sunter G., Gardiner W. E., Bisaro D. M. 1989; Identification of tomato golden mosaic virus-specific RNAs in infected plants. Virology 170:243–250 [CrossRef]
    [Google Scholar]
  48. Sunter G., Hartitz M. D., Bisaro D. M. 1993; Tomato golden mosaic virus leftward gene expression: autoregulation of geminivirus replication protein. Virology 195:275–280 [CrossRef]
    [Google Scholar]
  49. Sunter G., Sunter J. L., Bisaro D. M. 2001; Plants expressing Tomato golden mosaic virus AL2 or Beet curly top virus L2 transgenes show enhanced susceptibility to infection by DNA and RNA viruses. Virology 285:59–70 [CrossRef]
    [Google Scholar]
  50. Torres-Pacheco I., Garzón-Tiznado J. A., Herrera-Estrella L., Rivera-Bustamante R. F. 1993; Complete nucleotide sequence of Pepper huasteco virus : analysis and comparison with bipartite geminiviruses. J Gen Virol 74:2225–2231 [CrossRef]
    [Google Scholar]
  51. Townsend R., Stanley J., Curson S. J., Short M. N. 1985; Major polyadenylated transcripts of cassava latent virus and location of the gene encoding coat protein. EMBO J 4:33–37
    [Google Scholar]
  52. Wiley S. R., Kraus R. J., Zuo F. G., Murray E. E., Loritz K., Mertz J. E. 1993; SV40 early-to-late switch involves titration of cellular transcriptional repressors. Genes Dev 7:2206–2219 [CrossRef]
    [Google Scholar]
  53. Zhang Y. F., Wagner E. K. 1987; The kinetics of expression of individual herpes simplex virus type 1 transcripts. Virus Genes 1:49–60
    [Google Scholar]
  54. Zuo F., Mertz J. E. 1995; Simian virus 40 late gene expression is regulated by members of the steroid/thyroid hormone receptor superfamily. Proc Natl Acad Sci U S A 92:8586–8590 [CrossRef]
    [Google Scholar]
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