1887

Abstract

DNA is a circular single-stranded satellite DNA which co-infects with certain monopartite helper begomoviruses to cause economically important diseases, such as cotton leaf curl disease (CLCuD). DNA encodes a single protein, C1. Tomato leaf curl New Delhi virus (ToLCNDV) is a bipartite begomovirus in which both DNA A and DNA B are required for systemic infection. Inoculation of tomato plants with ToLCNDV DNA A alone induced local but not systemic infection, whereas co-inoculation with DNA A and the DNA associated with CLCuD resulted in systemic infection. DNA containing a disrupted C1 open reading frame (ORF) did not mobilize DNA A systemically. Co-inoculation of plants with DNA A and a construct of the C1 ORF, under the control of the cauliflower mosaic virus 35S promoter, resulted in the systemic movement of DNA A. In inoculated tobacco and onion epidermal cells, C1 fused to GFP was localized at the cell periphery in association with punctate bodies, around and within the cell nucleus and with the endoplasmic reticulum. It is concluded that heterologous C1 protein can replace the movement function of the DNA B of a bipartite begomovirus. Evidence is also provided that tomato leaf curl virus-encoded C4 protein confers the same movement function to ToLCNDV DNA A. The intracellular distribution of C1 is consistent with the hypothesis that it has a role in transporting the DNA A from the nuclear site of replication to the plasmodesmatal exit sites of the infected cell.

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2007-10-01
2024-03-29
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References

  1. Boisnard-Lorig C., Colon-Carmona A., Bauch M., Hodge S., Doerner P., Bancharel E., Dumas C., Haseloff J., Berger F. 2001; Dynamic analyses of the expression of the HISTONE : : YFP fusion protein in Arabidopsis show that syncytial endosperm is divided in mitotic domains. Plant Cell 13:495–509 [CrossRef]
    [Google Scholar]
  2. Briddon R. W., Stanley J. 2006; Subviral agents associated with plant single-stranded DNA viruses. Virology 344:198–210 [CrossRef]
    [Google Scholar]
  3. Briddon R. W., Mansoor S., Bedford I. D., Pinner M. S., Saunders K., Stanley J., Zafar Y., Malik K. A., Markham P. G. 2001; Identification of DNA components required for induction of cotton leaf curl disease. Virology 285:234–243 [CrossRef]
    [Google Scholar]
  4. Crete P., Leuenberger S., Iglesias V. A., Suarez V., Schob H., Holtorf H., van Eeden S., Meins F. 2001; Graft transmission of induced and spontaneous post-transcriptional silencing of chitinase genes. Plant J 28:493–501 [CrossRef]
    [Google Scholar]
  5. Cui X., Tao X., Xie Y., Fauquet C. M., Zhou X. 2004; A DNA β associated with Tomato yellow leaf curl China virus is required for symptom induction. J Virol 78:13966–13974 [CrossRef]
    [Google Scholar]
  6. Cui X., Li G., Wang D., Hu D., Zhou X. 2005; A begomovirus DNA β -encoded protein binds DNA, functions as a suppressor of RNA silencing, and targets the cell nucleus. J Virol 79:10764–10775 [CrossRef]
    [Google Scholar]
  7. Dry I. B., Rigden J. E., Krake L. R., Mullineaux P. M., Rezaian M. A. 1993; Nucleotide sequence and genome organization of tomato leaf curl geminivirus. J Gen Virol 74:147–151 [CrossRef]
    [Google Scholar]
  8. Gafni Y., Epel B. L., Harrison B. D. 2002; The role of host and viral proteins in intra- and inter-cellular trafficking of geminiviruses. Physiol Mol Plant Pathol 60:231–241 [CrossRef]
    [Google Scholar]
  9. Gilbertson R. L., Sudarshana M., Jiang H., Rojas M. R., Lucas W. J. 2003; Limitations on geminivirus genome size imposed by plasmodesmata and virus-encoded movement protein: insights into DNA trafficking. Plant Cell 15:2578–2591 [CrossRef]
    [Google Scholar]
  10. Gopal P., Kumar P. P., Sinilal B., Jose J., Yadunandam A. K., Usha R. 2007; Differential roles of C4 and β C1 in mediating suppression of post-transcriptional gene silencing: evidence for transactivation by the C2 of Bhendi yellow vein mosaic virus , a monopartite begomovirus. Virus Res 123:9–18 [CrossRef]
    [Google Scholar]
  11. Jeffrey J. L., Pooma W., Petty I. T. D. 1996; Genetic requirements for local and systemic movement of tomato golden mosaic virus in infected plants. Virology 223:208–218 [CrossRef]
    [Google Scholar]
  12. Jupin I., De Kouchkovsky F., Jouanneau F., Gronenborn B. 1994; Movement of tomato yellow leaf curl geminivirus (TYLCV): involvement of the protein encoded by ORF C4. Virology 204:82–90 [CrossRef]
    [Google Scholar]
  13. Kotlizky G., Boulton M. I., Pitaksutheepong C., Davies J. W., Epel B. L. 2000; Intracellular and intercellular movement of maize streak geminivirus V1 and V2 proteins transiently expressed as green fluorescent protein fusions. Virology 274:32–38 [CrossRef]
    [Google Scholar]
  14. Krake L. R., Rezaian M. A., Dry I. B. 1998; Expression of the tomato leaf curl geminivirus C4 gene produces viruslike symptoms in transgenic plants. Mol Plant Microbe Interact 11:413–417 [CrossRef]
    [Google Scholar]
  15. Kumar P. P., Usha R., Zrachya A., Levy Y., Spanov H., Gafni Y. 2006; Protein–protein interactions and nuclear trafficking of coat protein and β C1 protein associated with Bhendi yellow vein mosaic disease. Virus Res 122:127–136 [CrossRef]
    [Google Scholar]
  16. Levy A., Czosnek H. 2003; The DNA-B of the non-phloem-limited bean dwarf mosaic virus (BDMV) is able to move the phloem-limited Abutilon mosaic virus (AbMV) out of the phloem, but DNA-B of AbMV is unable to confine BDMV to the phloem. Plant Mol Biol 53:789–803 [CrossRef]
    [Google Scholar]
  17. Lucas W. J. 2006; Plant viral movement proteins: agents for cell-to-cell trafficking of viral genomes. Virology 344:169–184 [CrossRef]
    [Google Scholar]
  18. Mansoor S., Briddon R. W., Zafar Y., Stanley J. 2003; Geminivirus disease complexes: an emerging threat. Trends Plant Sci 8:128–134 [CrossRef]
    [Google Scholar]
  19. Mansoor S., Zafar Y., Briddon R. W. 2006; Geminivirus disease complexes: the threat is spreading. Trends Plant Sci 11:209–212 [CrossRef]
    [Google Scholar]
  20. Moffat A. S. 1999; Geminiviruses emerge as serious crop threat. Science 286:1835 [CrossRef]
    [Google Scholar]
  21. Padidam M., Beachy R. N., Fauquet C. M. 1995; Tomato leaf curl geminivirus from India has a bipartite genome and coat protein is not essential for infectivity. J Gen Virol 76:25–35 [CrossRef]
    [Google Scholar]
  22. Padidam M., Beachy R. N., Fauquet C. M. 1996; The role of AV2 (”precoat„ and coat protein in viral replication and movement in tomato leaf curl geminivirus. Virology 224:390–404 [CrossRef]
    [Google Scholar]
  23. Rigden J. E., Krake L. R., Rezaian M. A., Dry I. B. 1994; ORF C4 of tomato leaf curl geminivirus is a determinant of symptom severity. Virology 204:847–850 [CrossRef]
    [Google Scholar]
  24. Rojas M. R., Jiang H., Salati R., Xoconostle-Cazares B., Sudarshana M. R., Lucas W. J., Gilbertson R. L. 2001; Functional analysis of proteins involved in movement of the monopartite begomovirus, Tomato yellow leaf curl virus . Virology 291:110–125 [CrossRef]
    [Google Scholar]
  25. Rojas M. R., Hagen C., Lucas W. J., Gilbertson R. L. 2005; Exploiting chinks in the plant's armor: evolution and emergence of geminiviruses. Annu Rev Phytopathol 43:361–394 [CrossRef]
    [Google Scholar]
  26. Saeed M., Behjatnia S. A., Mansoor S., Zafar Y., Hasnain S., Rezaian M. A. 2005; A single complementary-sense transcript of a geminiviral DNA β satellite is determinant of pathogenicity. Mol Plant Microbe Interact 18:7–14 [CrossRef]
    [Google Scholar]
  27. Saunders K., Bedford I. D., Briddon R. W., Markham P. G., Wong S. M., Stanley J. 2000; A unique virus complex causes Ageratum yellow vein disease. Proc Natl Acad Sci U S A 97:6890–6895 [CrossRef]
    [Google Scholar]
  28. Saunders K., Salim N., Mali V. R., Malathi V. G., Briddon R., Markham P. G., Stanley J. 2002; Characterisation of Sri Lankan cassava mosaic virus and Indian cassava mosaic virus: evidence for acquisition of a DNA B component by a monopartite Begomovirus. Virology 293:63–74 [CrossRef]
    [Google Scholar]
  29. Saunders K., Norman A., Gucciardo S., Stanley J. 2004; The DNA β satellite component associated with Ageratum yellow vein disease encodes an essential pathogenicity protein ( β C1). Virology 324:37–47 [CrossRef]
    [Google Scholar]
  30. Scholthof H. B. 2005; Plant virus transport: motions of functional equivalence. Trends Plant Sci 10:376–382 [CrossRef]
    [Google Scholar]
  31. Schwach F., Vaistij F. E., Jones L., Baulcombe D. C. 2005; An RNA-dependent RNA polymerase prevents meristem invasion by potato virus X and is required for the activity but not the production of a systemic silencing signal. Plant Physiol 138:1842–1852 [CrossRef]
    [Google Scholar]
  32. Selth L. A., Dogra S. C., Rasheed M. S., Healy H., Randles J. W., Rezaian M. A. 2005; A NAC domain protein interacts with tomato leaf curl virus replication accessory protein and enhances viral replication. Plant Cell 17:311–325 [CrossRef]
    [Google Scholar]
  33. Seo Y. S., Gepts P., Gilbertson R. L. 2004; Genetics of resistance to the geminivirus, Bean dwarf mosaic virus , and the role of the hypersensitive response in common bean. Theor Appl Genet 108:786–793 [CrossRef]
    [Google Scholar]
  34. Stanley J., Bisaro D. M., Briddon R. W., Brown J. K., Fauquet C. M., Harrison B. D., Rybicki E. P., Stenger D. C. 2005; Geminiviridae . In Virus Taxonomy . VIIIth Report of the International Committee on Taxonomy of Viruses pp 301–326 Edited by Fauquet C. M., Mayo M. A., Maniloff J., Desselberger U., Ball L. A. London: Elsevier/Academic Press;
    [Google Scholar]
  35. Sudarshana M. R., Wang H. L., Lucas W. J., Gilbertson R. L. 1998; Dynamics of bean dwarf mosaic geminivirus cell-to-cell and long-distance movement in Phaseolus vulgaris revealed, using the green fluorescent protein. Mol Plant Microbe Interact 11:277–291 [CrossRef]
    [Google Scholar]
  36. Vanitharani R., Chellappan P., Fauquet C. M. 2005; Geminiviruses and RNA silencing. Trends Plant Sci 10:144–151 [CrossRef]
    [Google Scholar]
  37. Wang H. L., Sudarshana M. R., Gilbertson R. L., Lucas W. J. 1999; Analysis of cell-to-cell and long-distance movement of a bean dwarf mosaic geminivirus-green fluorescent protein reporter in host and nonhost species: identification of sites of resistance. Mol Plant Microbe Interact 12:345–355 [CrossRef]
    [Google Scholar]
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