%0 Journal Article %A Chang, Qing %A McLinden, James H. %A Stapleton, Jack T. %A Sathar, M. Aslam %A Xiang, Jinhua %T Expression of GB virus C NS5A protein from genotypes 1, 2, 3 and 5 and a 30 aa NS5A fragment inhibit human immunodeficiency virus type 1 replication in a CD4+ T-lymphocyte cell line %D 2007 %J Journal of General Virology, %V 88 %N 12 %P 3341-3346 %@ 1465-2099 %R https://doi.org/10.1099/vir.0.83198-0 %I Microbiology Society, %X GB virus type C (GBV-C) is a common human flavivirus that has been associated with prolonged survival in HIV-positive individuals in several, though not all, epidemiological studies. There are five distinct GBV-C genotypes that are geographically localized, and it has been speculated that GBV-C genotypic differences may explain variable outcomes observed in different clinical studies. Expression of an 85 aa fragment of the GBV-C NS5A phosphoprotein (genotype 2) in a CD4+ T cell line (Jurkat) resulted in inhibition of HIV replication, mediated in part by decreased surface expression of the HIV coreceptor CXCR4 and upregulation of SDF-1. We expressed the NS5A protein from genotypes 1, 2, 3 and 5 in Jurkat cells, and demonstrated that all genotypes inhibited HIV replication. Further deletion mapping demonstrated that expression of a 30 aa fragment resulted in decreased CXCR4 surface expression, upregulation of SDF-1 and inhibition of HIV replication. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.83198-0