RT Journal Article SR Electronic(1) A1 Zhai, You-gang A1 Lv, Xin-jun A1 Sun, Xiao-hong A1 Fu, Shi-hong A1 Gong, Zheng-da A1 Fen, Yun A1 Tong, Su-xiang A1 Wang, Zhao-xiao A1 Tang, Qing A1 Attoui, Houssam A1 Liang, Guo-dongYR 2008 T1 Isolation and characterization of the full coding sequence of a novel densovirus from the mosquito Culex pipiens pallens JF Journal of General Virology, VO 89 IS 1 SP 195 OP 199 DO https://doi.org/10.1099/vir.0.83221-0 PB Microbiology Society, SN 1465-2099, AB During an investigation of arboviruses in China, a novel densovirus (DNV) was isolated from the adult female Culex pipiens pallens. The virus, designated Culex pipiens pallens densovirus (CppDNV), caused cytopathic effect in C6/36 cells. The virus particles were icosahedral, non-enveloped and had a mean diameter of 24 nm. The complete coding region of CppDNV was found to be 3335 nt and it contained three open reading frames (ORFs). CppDNV shares 82–93 % identical nucleotides with isolates of the Aedes albopictus densovirus [isolates AalDNV-1, AalDNV-2 (C6/36 DNV) and AalDNV-3], Aedes aegypti densovirus (AaeDNV) and Haemagogus equines densovirus (HeDNV). The nucleotide sequence identity among CppDNV isolates exceeds 98 %. Phylogenetic trees based on non-structural (NS1 and NS2) and capsid (VP) genes show that CppDNV clustered with the species AaeDNV and represents a novel variant of this species within the genus Brevidensovirus., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.83221-0