1887

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Volume 88, Issue 12

Baculovirus envelope fusion proteins F and GP64 exploit distinct receptors to gain entry into cultured insect cells Free

Abstract

Group II nucleopolyhedroviruses (NPVs), e.g. (Hear) NPV and (Se) MNPV (multiple NPV), lack a GP64-like protein that is present in group I NPVs, e.g. (Ac)MNPV, but have an unrelated envelope fusion protein named F. Three AcMNPV viruses were constructed by introducing AcMNPV , HearNPV or SeMNPV genes, respectively, into a -negative AcMNPV bacmid. 21 cells were incubated with different amounts of inactivated budded virus to occupy receptors and were subsequently infected with a fixed amount of infectious virus to compete for attachment. The results suggest that GP64 and F act on their own and use different receptors, while the two different F proteins exploit the same receptor. Additionally, -null AcMNPV pseudotyped with baculovirus F was, in contrast to GP64, unable to transduce mammalian cells, indicating that mammalian cells do not possess baculovirus F protein receptors despite the structural similarity of baculovirus F to vertebrate viral fusion proteins.

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2007-12-01
2024-04-23
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Baculovirus envelope fusion proteins F and GP64 exploit distinct receptors to gain entry into cultured insect cells
J. Gen. Virol. 88, 3302 (2007); https://doi.org/10.1099/vir.0.83240-0
/content/journal/jgv/10.1099/vir.0.83240-0
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