1887

Abstract

European porcine H1N2 influenza viruses arose after multiple reassortment steps involving a porcine influenza virus with avian-influenza-like internal segments and human H1N1 and H3N2 viruses in 1994. In Germany, H1N2 swine influenza viruses first appeared in 2000. Two German H1N2 swine influenza virus strains isolated from pigs with clinical symptoms of influenza are described. They were characterized by the neutralization test, haemagglutination inhibition (HI) test and complete sequencing of the viral genomes. The data demonstrate that these viruses represent a novel H1N2 reassortant. The viruses showed limited neutralization by sera raised against heterologous A/sw/Bakum/1832/00-like H1N2 viruses. Sera pools from recovered pigs showed a considerably lower HI reaction, indicative of diagnostic difficulties in using the HI test to detect these viruses with A/sw/Bakum/1832/00-like H1N2 antigens. Genome sequencing revealed the novel combination of the human-like HAH1 gene of European porcine H1N2 influenza viruses and the NAN2 gene of European porcine H3N2 viruses.

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2008-01-01
2024-03-29
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References

  1. Brown I. H., Chakraverty P., Harris P. A., Alexander D. J. 1995; Disease outbreaks in pigs in Great Britain due to an influenza A virus of H1N2 subtype. Vet Rec 136:328–329 [CrossRef]
    [Google Scholar]
  2. Brown I. H., Ludwig S., Olsen C. W., Hannoun C., Scholtissek C., Hinshaw V. S., Harris P. A., McCauley J. W., Strong I., Alexander D. J. 1997; Antigenic and genetic analyses of H1N1 influenza A viruses from European pigs. J Gen Virol 78:553–562
    [Google Scholar]
  3. Campitelli L., Donatelli I., Foni E., Castrucci M. R., Fabiani C., Kawaoka Y., Krauss S., Webster R. G. 1997; Continued evolution of H1N1 and H3N2 influenza viruses in pigs in Italy. Virology 232:310–318 [CrossRef]
    [Google Scholar]
  4. Castrucci M. R., Donatelli I., Sidoli L., Barigazzi G., Kawaoka Y., Webster R. G. 1993; Genetic reassortment between avian and human influenza A viruses in Italian pigs. Virology 193:503–506 [CrossRef]
    [Google Scholar]
  5. Castrucci M. R., Campitelli L., Ruggieri A., Barigazzi G., Sidoli L., Daniels R., Oxford J. S., Donatelli I. 1994; Antigenic and sequence analysis of H3 influenza virus haemagglutinins from pigs in Italy. J Gen Virol 75:371–379 [CrossRef]
    [Google Scholar]
  6. Donatelli I., Campitelli L., Castrucci M. R., Ruggieri A., Sidoli L., Oxford J. S. 1991; Detection of two antigenic subpopulations of A (H1N1) influenza viruses from pigs: antigenic drift or interspecies transmission?. J Med Virol 34:248–257 [CrossRef]
    [Google Scholar]
  7. Kumar S., Tamura K., Nei M. 2004; mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163 [CrossRef]
    [Google Scholar]
  8. Nardelli L., Pascucci S., Gualandi G. L., Loda P. 1978; Outbreaks of classical swine influenza in Italy in 1976. Zentralbl Veterinarmed B 25:853–857
    [Google Scholar]
  9. Page R. D. M. 1996; TreeView: An application to display phylogenetic trees on personal computers. Comput Appl Biosci 12:357–358
    [Google Scholar]
  10. Palese P., Shaw M. L. 2007; Orthomyxoviridae : the viruses and their replication. In Fields Virology , 5th edn. vol 2 pp 1647–1689Edited by Knipe D. M., Howley P. M. Philadelphia: Lippincott Williams & Wilkins;
    [Google Scholar]
  11. Schmidtke M., Zell R., Bauer K., Krumbholz A., Schrader C., Suess J., Wutzler P. 2006; Amantadine resistance among porcine H1N1, H1N2, and H3N2 influenza A viruses isolated in Germany between 1981 and 2001. Intervirology 49:286–293 [CrossRef]
    [Google Scholar]
  12. Scholtissek C., Burger H., Bachmann P. A., Hannoun C. 1983; Genetic relatedness of hemagglutinins of the H1 subtype of influenza A viruses isolated from swine and birds. Virology 129:521–523 [CrossRef]
    [Google Scholar]
  13. Schrader C., Suess J. 2003; Genetic characterization of a porcine H1N2 influenza virus strain isolated in Germany. Intervirology 46:66–70 [CrossRef]
    [Google Scholar]
  14. Shope R. E. 1931; Swine influenza. I. Experimental transmission and pathology. J Exp Med 54:349–359 [CrossRef]
    [Google Scholar]
  15. Strimmer K., von Haeseler A. 1997; Likelihood-mapping: a simple method to visualize phylogenetic content of a sequence alignment. Proc Natl Acad Sci U S A 94:6815–6819 [CrossRef]
    [Google Scholar]
  16. Van Reeth K., Van Gucht S., Pensaert M. 2003; Investigations of the efficacy of European H1N1- and H3N2-based swine influenza vaccines against the novel H1N2 subtype. Vet Rec 153:9–13 [CrossRef]
    [Google Scholar]
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