1887

Abstract

Mutations in the surface glycoprotein (G) of infectious hematopoietic necrosis virus (IHNV), a rhabdovirus that causes significant losses in hatcheries raising salmonid fish, were studied. A 303 nt segment (mid-G region) of this protein from 88 Idaho isolates of IHNV was sequenced. Evidence of positive selection at individual codon sites was estimated by using a Bayesian method (MrBayes). A software algorithm (CPHmodels) was used to construct a three-dimensional (3D) representation of the IHNV protein. The software identified structural homologies between the IHNV G protein and the surface glycoprotein of vesicular stomatitis virus (VSV) and used the VSV structure as a template for predicting the IHNV structure. The amino acids predicted to be under positive selection were mapped onto the proposed IHNV 3D structure and appeared at sites on the surface of the protein where antigen–antibody interaction should be possible. The sites identified as being under positive selection on the IHNV protein corresponded to those reported by others as active sites of mutation for IHNV, and also as antigenic sites on VSV. Knowledge of the sites where genetic variation is positively selected enables a better understanding of the interaction of the virus with its host, and with the host immune system. This information could be used to develop strategies for vaccine development for IHNV, as well as for other viruses.

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2008-03-01
2024-04-19
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References

  1. Domingo E. 2006; Virus evolution. In Fields Virology . , 5th edn. pp 389–406Edited by Knipe D. M., Howley P. M. Baltimore, MD: Lippincott Williams & Wilkins;
  2. Emmenegger E. J., Meyers T. R., Burton T. O., Kurath G. 2000; Genetic diversity and epidemiology of infectious hematopoietic necrosis virus in Alaska. Dis Aquat Organ 40:163–176 [CrossRef]
    [Google Scholar]
  3. Huang C., Chien M. S., Landolt M., Batts W., Winton J. 1996; Mapping the neutralizing epitopes on the glycoprotein of infectious haematopoietic necrosis virus, a fish rhabdovirus. J Gen Virol 77:3033–3040 [CrossRef]
    [Google Scholar]
  4. Huelsenbeck J. P., Dyer K. A. 2004; Bayesian estimation of positively selected sites. J Mol Evol 58:661–672 [CrossRef]
    [Google Scholar]
  5. Huelsenbeck J. P., Jain S., Frost S. W. D., Kosakovsky-Pond S. L. 2006; A Dirichlet process model for detecting positive selection in protein-coding DNA sequences. Proc Natl Acad Sci U S A 103:6263–6268 [CrossRef]
    [Google Scholar]
  6. Kurath G., Garver K. A., Troyer R. M., Emmenegger E. J., Einer-Jensen K., Anderson E. D. 2003; Phylogeography of infectious haematopoietic necrosis virus in North America. J Gen Virol 84:803–814 [CrossRef]
    [Google Scholar]
  7. Lund O., Nielsen M., Lundegaard C., Worning P. 2002; CPHmodels 2.0: X3M a computer program to extract 3D models. In Abstracts of the CASP5 conference (the Fifth Community Wide Experiment on the Critical Assessment of Techniques for Protein Structure Prediction), Asilomar CA, USA 2002A102 http://www.cbs.dtu.dk/services/CPHmodels/abstract.php
    [Google Scholar]
  8. Nichol S. T., Rowe J. E., Winton J. R. 1995; Molecular epizootiology and evolution of the glycoprotein and non-virion protein genes of infectious hematopoietic necrosis virus, a fish rhabdovirus. Virus Res 38:159–173 [CrossRef]
    [Google Scholar]
  9. Novella I. S., Gilbertson D. L., Borrego B., Domingo E., Holland J. J. 2005; Adaptability costs in immune escape variants of vesicular stomatitis virus. Virus Res 107:27–34 [CrossRef]
    [Google Scholar]
  10. Oshima K. H., Arakawa C. K., Higman K. H., Landolt M. L., Nichol S. T., Winton J. R. 1995; The genetic diversity and epizootiology of infectious hematopoietic necrosis virus. Virus Res 35:123–141 [CrossRef]
    [Google Scholar]
  11. Roche S., Rey F., Gaudin Y., Bressanelli S. 2007; Structure of the prefusion form of the vesicular stomatitis virus glycoprotein G. Science 315:843–848 [CrossRef]
    [Google Scholar]
  12. Staden R., Beal K. F., Bonfield J. K. 2000; The Staden package, 1998. Methods Mol Biol 132:115–130
    [Google Scholar]
  13. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
    [Google Scholar]
  14. Tordo N., Benmansour A., Calisher C., Dietzgen R. G., Fang R.-X., Jackson A. O., Kurath G., Nadin-Davis S., Tesh R. B., Walker P. J. 2005; Family Rhabdoviridae . In Virus Taxonomy: Eighth Report of the International Committee on Taxonomy of Viruses pp 623–644Edited by Fauquet C., Mayo M. A., Maniloff J., Desselberger U., Ball L. A. San Diego, CA: Academic Press;
    [Google Scholar]
  15. Troyer R. M., Kurath G. 2003; Molecular epidemiology of infectious hematopoietic necrosis virus reveals complex virus traffic and evolution within Southern Idaho aquaculture. Dis Aquat Organ 55:175–185 [CrossRef]
    [Google Scholar]
  16. Troyer R. M., Lapatra S. E., Kurath G. 2000; Genetic analyses reveal unusually high diversity of infectious haematopoietic necrosis virus in rainbow trout aquaculture. J Gen Virol 81:2823–2832
    [Google Scholar]
  17. Vandepol S. B., Lefrancois L., Holland J. J. 1986; Sequences of the major antibody binding epitopes of the Indiana serotype of vesicular stomatitis virus. Virology 148:312–325 [CrossRef]
    [Google Scholar]
  18. Yang Z. 2007; paml 4: phylogenetic analysis by maximum likelihood. Mol Biol Evol 24:1586–1591 [CrossRef]
    [Google Scholar]
  19. Yang Z., Nielsen R., Goldman N., Pedersen A.-M. 2000; Codon-substitution models for heterogeneous selection pressure at amino acid sites. Genetics 155:431–449
    [Google Scholar]
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